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anti-Mouse (Murine) Leptin antibody for Enzyme Immunoassay

Recommended Leptin Antibody (supplied by: Log in to see )

Antigen
Leptin (LEP) Antibodies
  • LEPD
  • OB
  • ob
  • obese
  • OBS
Epitope
AA 57-70
24
22
21
13
13
11
9
8
6
6
6
6
6
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
442
151
131
18
3
1
1
Host
Sheep
350
244
20
8
3
Clonality
Polyclonal
Conjugate
This Leptin antibody is un-conjugated
66
25
19
13
9
9
7
6
6
6
6
6
6
6
6
6
6
6
6
5
5
3
3
3
3
3
3
2
2
Application
Enzyme Immunoassay (EIA)
446
412
71
59
40
33
25
25
18
17
16
13
10
7
5
3
3
3
3
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN318591
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008870071 ABIN115496 EIA WB Rabbit Log in to see Polyclonal 6
0.0008870071 ABIN115975 EIA WB Biotin Rabbit Log in to see Polyclonal
0.0008870071 ABIN115976 EIA WB Biotin Rabbit Log in to see Polyclonal
0.0008870071 ABIN115977 EIA IHC (p) WB Rabbit Log in to see Polyclonal
0.0008870071 ABIN115978 EIA IHC (p) WB Rabbit Log in to see Polyclonal
0.0008870071 ABIN401110 EIA WB Rabbit IgG Log in to see Polyclonal
0.0008870071 ABIN302917 EIA IHC (p) WB Rabbit Log in to see Polyclonal
0.0008870071 ABIN613249 EIA RIA Biotin Rabbit IgG Log in to see Polyclonal
0.0008870071 ABIN613723 EIA RIA Rabbit IgG Log in to see Polyclonal

Top referenced anti-Leptin antibody for Enzyme Immunoassay

Similar anti-Leptin Antibodies

Application / Reactivity Mouse (Murine) Human Rat (Rattus)
Western Blotting (WB) 92 Antibodies 289 Antibodies 75 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies
Radioimmunoassay (RIA) 6 Antibodies 2 Antibodies 2 Antibodies
Protein Assay (PrA) 3 Antibodies
Neutralization (Neut) 2 Antibodies 1 Antibodies
Multiplex Assay (MA) 1 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 19 Antibodies 6 Antibodies
Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 20 Antibodies 44 Antibodies 13 Antibodies

General

Antigen Leptin (LEP) Antibodies
Epitope AA 57-70
(24), (22), (21), (13), (13), (11), (9), (8), (6), (6), (6), (6), (6), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(442), (151), (131), (18), (3), (1), (1)
Host Sheep
(350), (244), (20), (8), (3)
Clonality
Polyclonal
Conjugate This Leptin antibody is un-conjugated
(66), (25), (19), (13), (9), (9), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (3), (3), (3), (3), (3), (3), (2), (2)
Application Enzyme Immunoassay (EIA)
(446), (412), (71), (59), (40), (33), (25), (25), (18), (17), (16), (13), (10), (7), (5), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Leptin Antibody

Target Details Leptin Application Details Handling References for anti-Leptin Antibody (ABIN318591) Images
Specificity This antibody recognises leptin.
Purification Purified
Immunogen HMW conjugated leptin amino acids 57-70.
Isotype IgG

Target Details Leptin

Product Details anti-Leptin Antibody Application Details Handling References for anti-Leptin Antibody (ABIN318591) Images back to top
Antigen
Alternative Name Leptin (LEP Antibody Abstract)
Background Leptin is a 16 kDa secreted protein hormone encoded by the OB gene, which plays a key role in the regulation of metabolism, body weight and reproduction and is expressed primarily by white adipocytes, and to a lesser extent by stomach endothelium and the placenta. Regulation of food intake and energy expenditure by leptin is thought to predominantly occur in the hypothalamus of the brain, where leptin binds with high affinity to neuronal leptin receptors (OB-R), inhibiting the action of the feeding stimulants neuropeptide Y (NPY) and agouti-related peptide (AgRP), or promoting the effects of the appetite regulator alpha-melanocyte-stimulating hormone (alpha-MSH). Leptin also acts as a direct stimulator of fatty acid oxidation in the mitochondria of liver and skeletal muscle cells, and influences glucose and fat metabolism. The expression of leptin in relation to metabolic status is influenced by hormonal and metabolite control: stimulators of leptin include insulin and cortisol, whilst cAMP, thiazolidinediones and beta-adrenergic receptor agonists, reduce leptin expression.Synonyms: LEP, OB, OBS, Obese protein, Obesity factor
Gene ID 3952
NCBI Accession NP_000221
UniProt P41159
Research Area Cardiovascular, Atherosclerosis, Hormones
Pathways JAK-STAT Signaling, AMPK Signaling

Application Details

Product Details anti-Leptin Antibody Target Details Leptin Handling References for anti-Leptin Antibody (ABIN318591) Images back to top
Application Notes ELISA: 1/10,000 - 1/50,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Leptin Antibody Target Details Leptin Application Details References for anti-Leptin Antibody (ABIN318591) Images back to top
Format Liquid
Concentration 5.0mg/mL
Buffer Phosphate buffered saline pH 7.4 containing 0.09 % Sodium Azide (NaN3)
Preservative Sodium azide
Precaution of Use This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage 4 °C
Storage Comment Store at +4 °C or at -20 °C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted.

References for anti-Leptin Antibody (ABIN318591)

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling Images back to top
Background publications

Mantzoros: "The role of leptin in human obesity and disease: a review of current evidence." in: Annals of internal medicine, Vol. 130, Issue 8, pp. 671-80, 1999