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anti-Mouse (Murine) Leptin antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Leptin Antibody (supplied by: Log in to see )

Antigen
Leptin (LEP) Antibodies
  • ob
  • obese
  • LEPD
  • OB
  • OBS
  • leptin
  • Lep
  • LEP
  • lep
Reactivity
Human, Mouse (Murine), Rat (Rattus)
414
167
143
22
6
4
4
4
3
2
2
2
2
1
1
1
1
1
1
Host
Rabbit
353
211
19
8
4
1
Clonality
Polyclonal
Conjugate
This Leptin antibody is un-conjugated
64
23
17
11
7
7
7
6
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
410
404
98
55
46
39
33
26
21
19
17
10
10
6
5
4
4
3
3
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN498515
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.701424 ABIN725030 IF (p) IHC (p) WB Rabbit IgG AA 90-130 Log in to see Polyclonal
11.701424 ABIN668243 IF (p) IHC (p) WB Rabbit IgG AA 1-50 Log in to see Polyclonal
11.701424 ABIN214037 IHC (p) ELISA WB Rabbit Log in to see Polyclonal
11.701424 ABIN4892220 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN604738 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN604739 IHC (p) WB Rabbit C-Term Log in to see Polyclonal
1 ABIN115977 EIA IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN115978 EIA IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN3201294 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 22-167 Log in to see
1 ABIN302917 EIA IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN205695 IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN725039 IHC (p) WB HRP Rabbit IgG AA 90-130 Log in to see Polyclonal
1 ABIN668252 IHC (p) WB HRP Rabbit IgG AA 1-50 Log in to see Polyclonal
1 ABIN1736401 IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN725032 IHC (p) WB Biotin Rabbit IgG AA 90-130 Log in to see Polyclonal
1 ABIN668245 IHC (p) WB Biotin Rabbit IgG AA 1-50 Log in to see Polyclonal
1 ABIN2892881 IHC (p) Rabbit Lys36 Log in to see Polyclonal
1 ABIN707495 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal
1 ABIN707488 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN707486 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal

Similar anti-Leptin Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies 1 Antibodies 1 Antibodies
Western Blotting (WB) 108 Antibodies 267 Antibodies 87 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies
Radioimmunoassay (RIA) 6 Antibodies 3 Antibodies 3 Antibodies
Protein Assay (PrA) 2 Antibodies
Neutralization (Neut) 2 Antibodies 1 Antibodies
Multiplex Assay (MA) 1 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 21 Antibodies 10 Antibodies 2 Antibodies

General

Antigen Leptin (LEP) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(414), (167), (143), (22), (6), (4), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(353), (211), (19), (8), (4), (1)
Clonality
Polyclonal
Conjugate This Leptin antibody is un-conjugated
(64), (23), (17), (11), (7), (7), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(410), (404), (98), (55), (46), (39), (33), (26), (21), (19), (17), (10), (10), (6), (5), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-Leptin Antibody

Target Details Leptin Application Details Handling Images
Specificity This detects endogenous levels of Ob protein. (region surrounding Lys36)
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Affinity chromatography using epitope-specific immunogen
Purity > 95 % (by SDS-PAGE)

Target Details Leptin

Product Details anti-Leptin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Leptin (LEP Antibody Abstract)
Background OB Cadherin is a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Expression of this particular cadherin in osteoblastic cell lines, and its upregulation during differentiation, suggests a specific function in bone development and maintenance. OB Cadherin is expressed in osteoblasts and the brain as well as other tissues.Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues.Synonyms: LEP, OB, OBS, Obese protein, Obesity factor
Gene ID 3952
NCBI Accession NP_000221
UniProt P41159
Research Area Cardiovascular, Atherosclerosis, Hormones
Pathways JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process

Application Details

Product Details anti-Leptin Antibody Target Details Leptin Handling Images back to top
Application Notes Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Leptin Antibody Target Details Leptin Application Details Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Leptin antibody (LEP) (ABIN498515) Immunohistochemistry (IHC) analyzes of Ob antibody in paraffin-embedded human ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Leptin antibody (LEP) (ABIN498515) Immunohistochemistry analyzes of Ob antibody in paraffin-embedded human breast...