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|Antigen||Leptin (LEP) Antibodies|
|Epitope||AA 57-70 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Leptin antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA)
|1 reference available|
|Supplier||Log in to see|
Product Details anti-Leptin AntibodyTarget Details Leptin Application Details Handling References for anti-Leptin antibody (ABIN318591) Images
|Specificity||This antibody recognises leptin.|
|Immunogen||HMW conjugated leptin amino acids 57-70.|
Target Details LeptinProduct Details anti-Leptin Antibody Application Details Handling References for anti-Leptin antibody (ABIN318591) Images back to top
|Alternative Name||Leptin (LEP Antibody Abstract)|
|Background||Leptin is a 16 kDa secreted protein hormone encoded by the OB gene, which plays a key role in the regulation of metabolism, body weight and reproduction and is expressed primarily by white adipocytes, and to a lesser extent by stomach endothelium and the placenta. Regulation of food intake and energy expenditure by leptin is thought to predominantly occur in the hypothalamus of the brain, where leptin binds with high affinity to neuronal leptin receptors (OB-R), inhibiting the action of the feeding stimulants neuropeptide Y (NPY) and agouti-related peptide (AgRP), or promoting the effects of the appetite regulator alpha-melanocyte-stimulating hormone (alpha-MSH). Leptin also acts as a direct stimulator of fatty acid oxidation in the mitochondria of liver and skeletal muscle cells, and influences glucose and fat metabolism. The expression of leptin in relation to metabolic status is influenced by hormonal and metabolite control: stimulators of leptin include insulin and cortisol, whilst cAMP, thiazolidinediones and beta-adrenergic receptor agonists, reduce leptin expression.Synonyms: LEP, OB, OBS, Obese protein, Obesity factor|
|Research Area||Cardiovascular, Atherosclerosis, Hormones|
|Pathways||JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process|
Application DetailsProduct Details anti-Leptin Antibody Target Details Leptin Handling References for anti-Leptin antibody (ABIN318591) Images back to top
ELISA: 1/10,000 - 1/50,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Leptin Antibody Target Details Leptin Application Details References for anti-Leptin antibody (ABIN318591) Images back to top
|Buffer||Phosphate buffered saline pH 7.4 containing 0.09 % Sodium Azide (NaN3)|
|Precaution of Use||This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.|
|Storage Comment||Store at +4 °C or at -20 °C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted.|
References for anti-Leptin antibody (ABIN318591)Product Details anti-Leptin Antibody Target Details Leptin Application Details Handling Images back to top
Mantzoros: "The role of leptin in human obesity and disease: a review of current evidence." in: Annals of internal medicine, Vol. 130, Issue 8, pp. 671-80, 1999