anti-Rat (Rattus) Leptin antibody for ELISA

Recommended Leptin Antibody (supplied by: Log in to see )

Antigen
Leptin (LEP) Antibodies
  • ob
  • obese
  • LEPD
  • OB
  • OBS
  • leptin
  • Lep
  • LEP
  • lep
Epitope
AA 22-167
30
24
18
15
15
15
11
6
6
6
6
6
6
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Rat (Rattus)
421
175
153
24
7
6
5
4
4
4
3
3
2
2
1
1
1
1
1
1
Host
Rabbit
372
209
19
8
4
1
Clonality
Polyclonal
Conjugate
This Leptin antibody is un-conjugated
64
26
17
14
10
7
7
7
6
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
Application
ELISA, Western Blotting (WB)
410
402
108
57
56
39
31
30
29
26
17
13
10
6
5
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN5518931
$ 240.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1169158 ELISA WB Mouse IgG2a Log in to see RLEP 227
1 ABIN108521 ELISA WB Rabbit Log in to see Polyclonal 4
1 ABIN108522 ELISA WB Rabbit Log in to see Polyclonal 6
1 ABIN1585862 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN1169153 ELISA WB Biotin Rabbit Log in to see Polyclonal
1 ABIN465028 ELISA WB Goat Log in to see Polyclonal
1 ABIN1169150 ELISA WB Rabbit Log in to see Polyclonal
1 ABIN1169161 ELISA WB Mouse IgG2a Log in to see RLEP 227
1 ABIN1822039 ELISA WB Goat IgG Log in to see Polyclonal
1 ABIN2884678 IHC ELISA Rabbit IgG AA 36-85 Log in to see Polyclonal
1 ABIN1584698 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN2612678 ELISA WB FITC Rabbit IgG AA 22-167 Log in to see Polyclonal
1 ABIN2612675 ELISA WB Biotin Rabbit IgG AA 22-167 Log in to see Polyclonal
1 ABIN2963299 ELISA WB Mouse IgG2a kappa Log in to see RLEP 227
1 ABIN1822632 ELISA Rabbit IgG Log in to see Polyclonal
1 ABIN465027 ELISA WB Biotin Goat Log in to see Polyclonal
1 ABIN2612671 ELISA WB Rabbit IgG AA 22-167 Log in to see Polyclonal
1 ABIN2612690 ELISA WB Rabbit Log in to see Polyclonal
1 ABIN2612691 ELISA WB Rabbit Log in to see Polyclonal
1 ABIN2612702 ELISA WB Mouse IgG2a, kappa Log in to see RLEP 227

General

Antigen Leptin (LEP) Antibodies
Epitope AA 22-167
(30), (24), (18), (15), (15), (15), (11), (6), (6), (6), (6), (6), (6), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Rat (Rattus)
(421), (175), (153), (24), (7), (6), (5), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(372), (209), (19), (8), (4), (1)
Clonality
Polyclonal
Conjugate This Leptin antibody is un-conjugated
(64), (26), (17), (14), (10), (7), (7), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application ELISA, Western Blotting (WB)
(410), (402), (108), (57), (56), (39), (31), (30), (29), (26), (17), (13), (10), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-Leptin Antibody

Target Details Leptin Application Details Handling Images
Purpose Rabbit IgG polyclonal antibody for Leptin(Lep) detection. Tested with WB, ELISA in Rat.
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Leptin(Lep) detection. Tested with WB, ELISA in Rat.
Gene Name: leptin
Protein Name: Leptin
Purification Immunogen affinity purified.
Immunogen E. coli-derived rat Leptin recombinant protein (Position: V22-C167). Rat Leptin shares 83.6% and 95.9% amino acid (aa) sequence identity with human and mouse Leptin, respectively.
Isotype IgG

Target Details Leptin

Product Details anti-Leptin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Lep (LEP Antibody Abstract)
Background Leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse, and rat cells. The expression of Leptin mRNA has been shown to be restricted to adipose tissue. Although regulation of fat stores is deemed to be the primary function of leptin, it also plays a role in other physiological processes, as evidenced by its multiple sites of synthesis other than fat cells, and the multiple cell types beside hypothalamic cells that have leptin receptors. Many of these additional functions are yet to be defined.

Synonyms: Leptin, Obesity factor, Lep, Ob
Gene ID 25608
UniProt P50596
Pathways JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process

Application Details

Product Details anti-Leptin Antibody Target Details Leptin Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat

Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Restrictions For Research Use only

Handling

Product Details anti-Leptin Antibody Target Details Leptin Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.