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|Antigen||Leptin (LEP) Antibodies|
|Epitope||AA 22-167 Alternatives|
|Reactivity||Rat (Rattus) Alternatives|
|Conjugate||This Leptin antibody is un-conjugated Alternatives|
ELISA, Western Blotting (WB)
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Product Details anti-Leptin AntibodyTarget Details Leptin Application Details Handling Images
|Purpose||Rabbit IgG polyclonal antibody for Leptin(Lep) detection. Tested with WB, ELISA in Rat.|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Leptin(Lep) detection. Tested with WB, ELISA in Rat.
Gene Name: leptin
Protein Name: Leptin
|Purification||Immunogen affinity purified.|
|Immunogen||E. coli-derived rat Leptin recombinant protein (Position: V22-C167). Rat Leptin shares 83.6% and 95.9% amino acid (aa) sequence identity with human and mouse Leptin, respectively.|
Target Details LeptinProduct Details anti-Leptin Antibody Application Details Handling Images back to top
|Alternative Name||Lep (LEP Antibody Abstract)|
Leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse, and rat cells. The expression of Leptin mRNA has been shown to be restricted to adipose tissue. Although regulation of fat stores is deemed to be the primary function of leptin, it also plays a role in other physiological processes, as evidenced by its multiple sites of synthesis other than fat cells, and the multiple cell types beside hypothalamic cells that have leptin receptors. Many of these additional functions are yet to be defined.
Synonyms: Leptin, Obesity factor, Lep, Ob
|Pathways||JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process|
Application DetailsProduct Details anti-Leptin Antibody Target Details Leptin Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Leptin Antibody Target Details Leptin Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C,-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.