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anti-Cow (Bovine) PIAS3 antibody for Western Blotting

Recommended PIAS3 Antibody (supplied by: Log in to see )

Antigen
Protein Inhibitor of Activated STAT, 3 (PIAS3) Antibodies
  • PIAS3
  • Pias3l
  • ZMIZ5
Epitope
Middle Region
17
10
3
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Rat (Rattus)
60
25
24
4
3
2
1
Host
Rabbit
56
4
Clonality
Polyclonal
Application
Western Blotting (WB)
52
35
30
11
5
2
2
1
1
Supplier
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Catalog No. ABIN2777575
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.1270541 ABIN2777574 IHC WB Rabbit C-Term Log in to see Polyclonal 1
0.1270541 ABIN2707703 IHC WB Rabbit N-Term Log in to see Polyclonal

Top referenced anti-PIAS3 antibody for Western Blotting

Similar anti-PIAS3 Antibodies

Application / Reactivity Human Rat (Rattus) Cow (Bovine) Dog (Canine)
Western Blotting (WB) 54 Antibodies 24 Antibodies 3 Antibodies 4 Antibodies
RNA Interference (RNAi) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies 5 Antibodies
Immunohistochemistry (IHC) 31 Antibodies 2 Antibodies 3 Antibodies 16 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunofluorescence (IF) 5 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
ELISA 35 Antibodies

General

Antigen Protein Inhibitor of Activated STAT, 3 (PIAS3) Antibodies
Epitope Middle Region
(17), (10), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Rat (Rattus)
(60), (25), (24), (4), (3), (2), (1)
Host Rabbit
(56), (4)
Clonality
Polyclonal
Application Western Blotting (WB)
(52), (35), (30), (11), (5), (2), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-PIAS3 Antibody

Target Details PIAS3 Application Details Handling References for anti-PIAS3 Antibody (ABIN2777575) Images
Blocking Peptide Blocking peptide for this product available: ABIN981734
Sequence DEIQFMEDGS WCPMKPKKEA SEVCPPPGYG LDGLQYSPVQ GGDPSENKKK
Predicted Reactivity Human : 100 %, Dog : 80 %, Guinea pig : 80 %, Horse : 80 %, Bovine : 70 %, Rabbit : 70 %, Rat : 70 %
Characteristics This is a rabbit polyclonal antibody against PIAS3. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human PIAS3

Target Details PIAS3

Product Details anti-PIAS3 Antibody Application Details Handling References for anti-PIAS3 Antibody (ABIN2777575) Images back to top
Antigen
Alternative Name PIAS3 (PIAS3 Antibody Abstract)
Background PIAS3 is a member of the protein inhibitor of activated STAT (PIAS) family. It also activates TGF-beta/SMAD transcriptional responses. This gene encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined.
Molecular Weight 68 kDa
Gene ID 10401
NCBI Accession NM_006099, NP_006090
UniProt Q9Y6X2
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Pathways JAK-STAT Signaling

Application Details

Product Details anti-PIAS3 Antibody Target Details PIAS3 Handling References for anti-PIAS3 Antibody (ABIN2777575) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 628 AA

Restrictions For Research Use only

Handling

Product Details anti-PIAS3 Antibody Target Details PIAS3 Application Details References for anti-PIAS3 Antibody (ABIN2777575) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-PIAS3 Antibody (ABIN2777575)

Product Details anti-PIAS3 Antibody Target Details PIAS3 Application Details Handling Images back to top
Background publications

Roukens, Alloul-Ramdhani, Vertegaal, Anvarian, Balog, Deelder, Hensbergen, Baker: "Identification of a new site of sumoylation on Tel (ETV6) uncovers a PIAS-dependent mode of regulating Tel function." in: Molecular and cellular biology, Vol. 28, Issue 7, pp. 2342-57, 2008

Images

Product Details anti-PIAS3 Antibody Target Details PIAS3 Application Details Handling References for anti-PIAS3 Antibody (ABIN2777575) back to top
Supplier Images
Western Blotting (WB) image for anti-PIAS3 antibody (Protein Inhibitor of Activated STAT, 3) (Middle Region) (ABIN2777575) anti-Protein Inhibitor of Activated STAT, 3 (PIAS3) (Middle Region) antibody