You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human PIAS3 antibody for Enzyme Immunoassay

Recommended PIAS3 Antibody (supplied by: Log in to see )

Antigen
Protein Inhibitor of Activated STAT, 3 (PIAS3) Antibodies
  • PIAS3
  • Pias3l
  • ZMIZ5
Epitope
C-Term
17
10
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
62
25
25
5
4
2
1
Host
Rabbit
58
4
Clonality
Polyclonal
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
53
36
32
12
5
2
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN500491
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN783253 EIA IHC (p) WB Rabbit C-Term Log in to see Polyclonal

Top referenced anti-PIAS3 antibody for Enzyme Immunoassay

Similar anti-PIAS3 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 55 Antibodies 25 Antibodies 25 Antibodies
RNA Interference (RNAi) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 13 Antibodies 6 Antibodies 6 Antibodies
Immunohistochemistry (IHC) 33 Antibodies 17 Antibodies 17 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunofluorescence (IF) 5 Antibodies 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies

General

Antigen Protein Inhibitor of Activated STAT, 3 (PIAS3) Antibodies
Epitope C-Term
(17), (10), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(62), (25), (25), (5), (4), (2), (1)
Host Rabbit
(58), (4)
Clonality
Polyclonal
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(53), (36), (32), (12), (5), (2), (1), (1), (1)
Pubmed 2 references available
Supplier Log in to see

Product Details anti-PIAS3 Antibody

Target Details PIAS3 Application Details Handling References for anti-PIAS3 Antibody (ABIN500491) Images
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification Peptide affinity chromatography
Immunogen PIAS3 antibody was raised against a 12 amino acid peptide near the carboxy terminus of human PIAS3.
Isotype IgG

Target Details PIAS3

Product Details anti-PIAS3 Antibody Application Details Handling References for anti-PIAS3 Antibody (ABIN500491) Images back to top
Antigen
Alternative Name PIAS3 (PIAS3 Antibody Abstract)
Background The PIAS (protein inhibitor of activated STAT) proteins play a crucial role as transcriptional coregulators in various cellular pathways, including the STAT, p53 and the steroid hormone signaling pathway. The PIAS protein family includes at least five evolutionarily conserved genes, including PIAS3. The major function of the PIAS proteins is the control of gene transcription and can also act as small ubiquitin-like-modifier (SUMO) E3 ligases. PIAS3 binds specifically to STAT3 following the stimulation of STAT3. Increased expression of PIAS3 has been observed in several human cancers, including lung, breast, and brain tumors, but not in anaplastic lymphoma kinase-positive T/null-cell lymphomas, indicating that PIAS3 plays multiple roles in different tissue and cell types.Synonyms: E3 SUMO-protein ligase PIAS3, Protein inhibitor of activated STAT protein 3
Gene ID 10401
NCBI Accession NP_006090
UniProt Q9Y6X2
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Pathways JAK-STAT Signaling

Application Details

Product Details anti-PIAS3 Antibody Target Details PIAS3 Handling References for anti-PIAS3 Antibody (ABIN500491) Images back to top
Application Notes ELISA. Western blot: 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PIAS3 Antibody Target Details PIAS3 Application Details References for anti-PIAS3 Antibody (ABIN500491) Images back to top
Concentration 1.0 mg/mL
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.

References for anti-PIAS3 Antibody (ABIN500491)

Product Details anti-PIAS3 Antibody Target Details PIAS3 Application Details Handling Images back to top
Background publications

Shuai, Liu: "Regulation of gene-activation pathways by PIAS proteins in the immune system." in: Nature reviews. Immunology, Vol. 5, Issue 8, pp. 593-605, 2005

Chung, Liao, Liu, Rao, Jay, Berta, Shuai: "Specific inhibition of Stat3 signal transduction by PIAS3." in: Science (New York, N.Y.), Vol. 278, Issue 5344, pp. 1803-5, 1997