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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||54 Antibodies||24 Antibodies||24 Antibodies|
|RNA Interference (RNAi)||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||12 Antibodies||5 Antibodies||5 Antibodies|
|Immunohistochemistry (IHC)||31 Antibodies||16 Antibodies||16 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunofluorescence (IF)||5 Antibodies||2 Antibodies||2 Antibodies|
|Immunocytochemistry (ICC)||2 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||2 Antibodies|
|Antigen||Protein Inhibitor of Activated STAT, 3 (PIAS3) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|2 references available|
|Supplier||Log in to see|
Product Details anti-PIAS3 AntibodyTarget Details PIAS3 Application Details Handling References for anti-PIAS3 Antibody (ABIN500491) Images
|Cross-Reactivity (Details)||Species reactivity (tested):Human, mouse, rat|
|Purification||Peptide affinity chromatography|
|Immunogen||PIAS3 antibody was raised against a 12 amino acid peptide near the carboxy terminus of human PIAS3.|
Target Details PIAS3Product Details anti-PIAS3 Antibody Application Details Handling References for anti-PIAS3 Antibody (ABIN500491) Images back to top
|Alternative Name||PIAS3 (PIAS3 Antibody Abstract)|
|Background||The PIAS (protein inhibitor of activated STAT) proteins play a crucial role as transcriptional coregulators in various cellular pathways, including the STAT, p53 and the steroid hormone signaling pathway. The PIAS protein family includes at least five evolutionarily conserved genes, including PIAS3. The major function of the PIAS proteins is the control of gene transcription and can also act as small ubiquitin-like-modifier (SUMO) E3 ligases. PIAS3 binds specifically to STAT3 following the stimulation of STAT3. Increased expression of PIAS3 has been observed in several human cancers, including lung, breast, and brain tumors, but not in anaplastic lymphoma kinase-positive T/null-cell lymphomas, indicating that PIAS3 plays multiple roles in different tissue and cell types.Synonyms: E3 SUMO-protein ligase PIAS3, Protein inhibitor of activated STAT protein 3|
|Research Area||Chromatin and Nuclear Signaling, Transcription Factors|
Application DetailsProduct Details anti-PIAS3 Antibody Target Details PIAS3 Handling References for anti-PIAS3 Antibody (ABIN500491) Images back to top
ELISA. Western blot: 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PIAS3 Antibody Target Details PIAS3 Application Details References for anti-PIAS3 Antibody (ABIN500491) Images back to top
|Buffer||PBS containing 0.02 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store the antibody (in aliquots) at -20 °C.|
References for anti-PIAS3 Antibody (ABIN500491)Product Details anti-PIAS3 Antibody Target Details PIAS3 Application Details Handling Images back to top
Shuai, Liu: "Regulation of gene-activation pathways by PIAS proteins in the immune system." in: Nature reviews. Immunology, Vol. 5, Issue 8, pp. 593-605, 2005 (PubMed).
Chung, Liao, Liu et al.: "Specific inhibition of Stat3 signal transduction by PIAS3." in: Science (New York, N.Y.), Vol. 278, Issue 5344, pp. 1803-5, 1997 (PubMed).