anti-Rat (Rattus) PIAS3 antibody for Western Blotting

Recommended PIAS3 Antibody (supplied by: Log in to see )

Antigen
Protein Inhibitor of Activated STAT, 3 (PIAS3) Antibodies
  • PIAS3
  • Pias3l
  • ZMIZ5
  • protein inhibitor of activated STAT, 3
  • protein inhibitor of activated STAT 3
  • PIAS3
  • pias3
  • Pias3
  • LOC100341019
Epitope
Middle Region
16
10
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Rabbit, Rat (Rattus)
65
30
28
7
6
5
3
2
1
1
Host
Rabbit
61
4
Clonality
Polyclonal
Application
Western Blotting (WB)
58
35
31
15
6
2
2
2
1
1
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Catalog No. ABIN2777575
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.3487515 ABIN2777574 IHC WB Rabbit C-Term Log in to see Polyclonal 1
3.3487515 ABIN1533434 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.3487515 ABIN2707703 IHC WB Rabbit N-Term Log in to see Polyclonal
3.3487515 ABIN783253 EIA IHC (p) WB Rabbit C-Term Log in to see Polyclonal
3.3487515 ABIN3186437 ELISA IHC WB Rabbit IgG N-Term Log in to see Polyclonal
3.3487515 ABIN4345426 ELISA IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal
3.3487515 ABIN1030584 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
3.3487515 ABIN1848836 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN271953 IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN5555120 EIA WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN202823 IHC (p) WB Rabbit IgG AA 548-597 Log in to see Polyclonal
1 ABIN467799 WB Rabbit AA 395-444 Log in to see Polyclonal
1 ABIN2879031 IHC (p) ELISA WB Rabbit IgG AA 10-59 Log in to see Polyclonal
1 ABIN605174 IHC (p) ELISA WB Rabbit C-Term Log in to see Polyclonal
1 ABIN1738082 IHC (p) ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN1738083 IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN2891709 IHC (p) WB Rabbit Phe25 Log in to see Polyclonal
1 ABIN2626790 IHC (p) WB Rabbit N-Term Log in to see Polyclonal
1 ABIN2882958 IF IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN2861225 IF IHC ELISA WB Rabbit C-Term Log in to see Polyclonal

Similar anti-PIAS3 Antibodies

Application / Reactivity Human Rat (Rattus) Cow (Bovine) Dog (Canine) Horse (Equine) Guinea Pig Rabbit
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Western Blotting (WB) 59 Antibodies 29 Antibodies 6 Antibodies 7 Antibodies 4 Antibodies 3 Antibodies 2 Antibodies
RNA Interference (RNAi) 1 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 15 Antibodies 10 Antibodies 2 Antibodies 2 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 31 Antibodies 2 Antibodies 3 Antibodies 15 Antibodies 1 Antibodies

General

Antigen Protein Inhibitor of Activated STAT, 3 (PIAS3) Antibodies
Epitope Middle Region
(16), (10), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Rabbit, Rat (Rattus)
(65), (30), (28), (7), (6), (5), (3), (2), (1), (1)
Host Rabbit
(61), (4)
Clonality
Polyclonal
Application Western Blotting (WB)
(58), (35), (31), (15), (6), (2), (2), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-PIAS3 Antibody

Target Details PIAS3 Application Details Handling ProductDetails: References for anti-PIAS3 antibody (ABIN2777575) Images
Blocking Peptide Blocking peptide for this product available: ABIN981734
Sequence DEIQFMEDGS WCPMKPKKEA SEVCPPPGYG LDGLQYSPVQ GGDPSENKKK
Predicted Reactivity Cow: 79%, Dog: 86%, Guinea Pig: 86%, Horse: 86%, Human: 100%, Rabbit: 79%, Rat: 79%
Characteristics This is a rabbit polyclonal antibody against PIAS3. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human PIAS3

Target Details PIAS3

Product Details anti-PIAS3 Antibody Application Details Handling ProductDetails: References for anti-PIAS3 antibody (ABIN2777575) Images back to top
Antigen
Alternative Name PIAS3 (PIAS3 Antibody Abstract)
Background PIAS3 is a member of the protein inhibitor of activated STAT (PIAS) family. It also activates TGF-beta/SMAD transcriptional responses. This gene encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: FLJ14651, ZMIZ5
Protein Interaction Partner: UBC, HIC1, TRIM63, TRIM55, TAB2, SUMO1, CBS, SUMO2, SUMO3, STAT3, NCOA2, CREM, PRPF40A, SERPINA10, GEMIN4, UBE2I, SKIL, HNRNPK, ZFHX3, SERBP1, SATB1, ERBB4, Bcl11a, ELAVL1, PPP1CA, Grm7, Grm8, Grm6, Grm4, TRIM8, RAC1, C19orf60, CARHSP1, UBA1, PSMC1, TRIM2
Protein Size: 628
Molecular Weight 68 kDa
Gene ID 10401
NCBI Accession NM_006099, NP_006090
UniProt Q9Y6X2
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Pathways JAK-STAT Signaling

Application Details

Product Details anti-PIAS3 Antibody Target Details PIAS3 Handling ProductDetails: References for anti-PIAS3 antibody (ABIN2777575) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 628 AA

Restrictions For Research Use only

Handling

Product Details anti-PIAS3 Antibody Target Details PIAS3 Application Details ProductDetails: References for anti-PIAS3 antibody (ABIN2777575) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

ProductDetails: References for anti-PIAS3 antibody (ABIN2777575)

Product Details anti-PIAS3 Antibody Target Details PIAS3 Application Details Handling Images back to top
Background publications

Roukens, Alloul-Ramdhani, Vertegaal, Anvarian, Balog, Deelder, Hensbergen, Baker: "Identification of a new site of sumoylation on Tel (ETV6) uncovers a PIAS-dependent mode of regulating Tel function." in: Molecular and cellular biology, Vol. 28, Issue 7, pp. 2342-57, 2008

Images

Product Details anti-PIAS3 Antibody Target Details PIAS3 Application Details Handling ProductDetails: References for anti-PIAS3 antibody (ABIN2777575) back to top
Supplier Images
Western Blotting (WB) image for anti-PIAS3 antibody (Protein Inhibitor of Activated STAT, 3) (Middle Region) (ABIN2777575) anti-Protein Inhibitor of Activated STAT, 3 (PIAS3) (Middle Region) antibody