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anti-Human BRAF antibody for Dot Blot

Recommended BRAF Antibody (supplied by: Log in to see )

Antigen
V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
  • 9930012E13Rik
  • AA120551
  • AA387315
  • AA473386
  • B-raf
  • B-Raf
  • B-RAF1
  • b-raf1
  • Braf
  • BRAF
  • Braf-2
  • BRAF1
  • braf1
  • Braf2
  • C-RMIL
  • C87398
  • C230098H17
  • D6Ertd631e
  • HYPT11
  • NS7
  • rafb1
  • RAFB1
  • RMIL
  • sierrabraf
  • Sm-E
  • SME
Epitope
pThr400
29
24
23
23
21
17
17
17
15
14
13
13
9
8
7
6
5
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
376
51
44
6
4
1
1
1
Host
Rabbit
284
89
2
2
Clonality
Polyclonal
Conjugate
This BRAF antibody is un-conjugated
20
20
20
17
17
17
3
3
3
3
3
3
3
3
3
Application
Dot Blot (DB)
249
212
150
82
43
41
33
16
4
4
2
1
1
1
Supplier
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Catalog No. ABIN389897
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.61167514 ABIN389701 DB Rabbit Ig pThr439 Log in to see Polyclonal 2
0.61167514 ABIN389791 DB Rabbit Ig pThr598 Log in to see Polyclonal
0.61167514 ABIN389803 DB Rabbit Ig pSer445 Log in to see Polyclonal
0.61167514 ABIN2159586 DB Rabbit Ig Fraction pThr400 Log in to see Polyclonal
0.61167514 ABIN2159585 DB Rabbit Ig Fraction pThr439 Log in to see Polyclonal
0.61167514 ABIN2157912 DB ELISA Rabbit Ig Fraction Log in to see Polyclonal
0.61167514 ABIN2157913 DB ELISA Rabbit Ig Fraction Log in to see Polyclonal
0.0008893085 ABIN1952913 DB ELISA Alkaline Phosphatase (AP) Rabbit IgG Ser445 Log in to see Polyclonal
0.0008893085 ABIN1952951 DB ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Ser601 Log in to see Polyclonal
0.0008893085 ABIN1953110 DB ELISA Alkaline Phosphatase (AP) Rabbit IgG Thr400 Log in to see Polyclonal
0.0008893085 ABIN1953114 DB ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Thr439 Log in to see Polyclonal
0.0008893085 ABIN1953131 DB ELISA Alkaline Phosphatase (AP) Rabbit IgG Thr598 Log in to see Polyclonal
0.0008893085 ABIN1953785 DB ELISA APC Rabbit IgG Ser445 Log in to see Polyclonal
0.0008893085 ABIN1953823 DB ELISA WB APC Rabbit IgG Ser601 Log in to see Polyclonal
0.0008893085 ABIN1953982 DB ELISA APC Rabbit IgG Thr400 Log in to see Polyclonal
0.0008893085 ABIN1953986 DB ELISA WB APC Rabbit IgG Thr439 Log in to see Polyclonal
0.0008893085 ABIN1954003 DB ELISA APC Rabbit IgG Thr598 Log in to see Polyclonal
0.0008893085 ABIN1956227 DB ELISA FITC Rabbit IgG Ser445 Log in to see Polyclonal
0.0008893085 ABIN1956264 DB ELISA WB FITC Rabbit IgG Ser601 Log in to see Polyclonal
0.0008893085 ABIN1956419 DB ELISA FITC Rabbit IgG Thr400 Log in to see Polyclonal

Top referenced anti-BRAF antibody for Dot Blot

  • Human Polyclonal BRAF Primary Antibody for DB - ABIN389897 (3 Publications): Frattini, Ferrario, Bressan, Balestra, De Cecco, Mondellini, Bongarzone, Collini, Gariboldi, Pilotti, Pierotti, Greco: Alternative mutations of BRAF, RET and NTRK1 are associated with similar but distinct gene expression patterns in papillary thyroid cancer. in Oncogene 2004 (PubMed)

Similar anti-BRAF Antibodies

Application / Reactivity Human
Dot Blot (DB) 83 Antibodies
ELISA 211 Antibodies
ELISA (Capture) 1 Antibodies
Flow Cytometry (FACS) 17 Antibodies
Functional Studies (Func) 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 40 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies
Immunohistochemistry (IHC) 151 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 44 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Luminex Assay (LMNX) 4 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 249 Antibodies

General

Antigen V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
Epitope pThr400
(29), (24), (23), (23), (21), (17), (17), (17), (15), (14), (13), (13), (9), (8), (7), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(376), (51), (44), (6), (4), (1), (1), (1)
Host Rabbit
(284), (89), (2), (2)
Clonality (Clone)
Polyclonal   ( )
Conjugate This BRAF antibody is un-conjugated
(20), (20), (20), (17), (17), (17), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Dot Blot (DB)
(249), (212), (150), (82), (43), (41), (33), (16), (4), (4), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-BRAF Antibody

Target Details BRAF Application Details Handling References for anti-BRAF Antibody (ABIN389897) Images
Specificity This Phospho-BRAF-pT400 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T400 of human BRAF.
Predicted Reactivity Mouse (Murine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This BRAF Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T400 of human BRAF.
Clone RB13721
Isotype Ig

Target Details BRAF

Product Details anti-BRAF Antibody Application Details Handling References for anti-BRAF Antibody (ABIN389897) Images back to top
Antigen
Alternative Name BRAF (BRAF Antibody Abstract)
Background BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome), and a wide range of cancers such as lung cancer, non-Hodgkins lymphoma, and colorectal cancer.
Synonyms: B-Raf proto-oncogene serine/threonine-protein kinase, p94, v-Raf murine sarcoma viral oncogene homolog B1
Molecular Weight 84437 DA
Gene ID 673
UniProt P15056
Research Area Cell Structure, Phospho-specific antibodies
Pathways MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-BRAF Antibody Target Details BRAF Handling References for anti-BRAF Antibody (ABIN389897) Images back to top
Application Notes DB = 1:500
Restrictions For Research Use only

Handling

Product Details anti-BRAF Antibody Target Details BRAF Application Details References for anti-BRAF Antibody (ABIN389897) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-BRAF Antibody (ABIN389897)

Product Details anti-BRAF Antibody Target Details BRAF Application Details Handling Images back to top
Product cited in:

Duan, Cobb: "Calcineurin increases glucose activation of ERK1/2 by reversing negative feedback." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 51, pp. 22314-9, 2010

Background publications

Frattini, Ferrario, Bressan, Balestra, De Cecco, Mondellini, Bongarzone, Collini, Gariboldi, Pilotti, Pierotti, Greco: "Alternative mutations of BRAF, RET and NTRK1 are associated with similar but distinct gene expression patterns in papillary thyroid cancer." in: Oncogene, Vol. 23, Issue 44, pp. 7436-40, 2004

Tsavachidou, Coleman, Athanasiadis, Li, Licht, Olson, Weber: "SPRY2 is an inhibitor of the ras/extracellular signal-regulated kinase pathway in melanocytes and melanoma cells with wild-type BRAF but not with the V599E mutant." in: Cancer research, Vol. 64, Issue 16, pp. 5556-9, 2004

Images

Product Details anti-BRAF Antibody Target Details BRAF Application Details Handling References for anti-BRAF Antibody (ABIN389897) back to top
Supplier Images
Dot Blot (DB) image for anti-BRAF antibody (V-Raf Murine Sarcoma Viral Oncogene Homolog B1) (pThr400) (ABIN389897) Dot blot analysis of anti-BRAF Phospho-specific Pab (ABIN389897) on nitrocellulose me...