anti-Human BRAF antibody for Dot Blot

Recommended BRAF Antibody (supplied by: Log in to see )

Antigen
V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
  • B-Raf
  • braf1
  • rafb1
  • b-raf1
  • sierrabraf
  • BRAF
  • 9930012E13Rik
  • AA120551
  • AA387315
  • AA473386
  • B-raf
  • Braf-2
  • Braf2
  • C230098H17
  • C87398
  • D6Ertd631e
  • Braf
  • HYPT11
  • SME
  • Sm-E
  • B-RAF1
  • BRAF1
  • NS7
  • RAFB1
  • C-RMIL
  • RMIL
  • v-raf murine sarcoma viral oncogene homolog B
  • v-raf murine sarcoma viral oncogene homolog B1
  • Braf transforming gene
  • Braf transforming gene, related sequence 1
  • small nuclear ribonucleoprotein polypeptide E
  • braf
  • BRAF
  • LOC100219601
  • Braf
  • Braf-rs1
  • SNRPE
Epitope
pThr439
36
33
29
24
22
21
20
18
16
13
11
10
10
9
8
6
5
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
406
85
69
13
10
4
4
3
1
1
1
1
Host
Rabbit
308
94
3
2
Clonality
Polyclonal
Conjugate
This BRAF antibody is un-conjugated
20
20
20
17
17
17
3
3
3
3
3
3
3
3
3
3
3
Application
Dot Blot (DB)
268
210
145
77
71
45
39
22
11
8
5
4
1
1
1
1
Options
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Catalog No. ABIN389701
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
28.674713 ABIN389897 DB Rabbit Ig pThr400 Log in to see Polyclonal 3
28.674713 ABIN389791 DB Rabbit Ig pThr598 Log in to see Polyclonal
28.674713 ABIN389803 DB Rabbit Ig pSer445 Log in to see Polyclonal
28.674713 ABIN3029547 DB ELISA Rabbit Ig Fraction pSer445 Log in to see Polyclonal
28.674713 ABIN3029548 DB ELISA Rabbit Ig Fraction pThr400 Log in to see Polyclonal
28.674713 ABIN3029549 DB ELISA Rabbit Ig Fraction pThr439 Log in to see Polyclonal
1 ABIN1952951 DB ELISA FACS IHC IP WB Alkaline Phosphatase (AP) Rabbit IgG pSer601 Log in to see Polyclonal
1 ABIN1953110 DB ELISA Alkaline Phosphatase (AP) Rabbit IgG pThr400 Log in to see Polyclonal
1 ABIN1953114 DB ELISA WB Alkaline Phosphatase (AP) Rabbit IgG pThr439 Log in to see Polyclonal
1 ABIN1953131 DB ELISA Alkaline Phosphatase (AP) Rabbit IgG pThr598 Log in to see Polyclonal
1 ABIN1953785 DB ELISA APC Rabbit IgG pSer445 Log in to see Polyclonal
1 ABIN1953823 DB ELISA FACS IHC IP WB APC Rabbit IgG pSer601 Log in to see Polyclonal
1 ABIN1953982 DB ELISA APC Rabbit IgG pThr400 Log in to see Polyclonal
1 ABIN1953986 DB ELISA WB APC Rabbit IgG pThr439 Log in to see Polyclonal
1 ABIN1954003 DB ELISA APC Rabbit IgG pThr598 Log in to see Polyclonal
1 ABIN1955062 DB ELISA Biotin Rabbit IgG pSer445 Log in to see Polyclonal
1 ABIN1955095 DB ELISA FACS IHC IP WB Biotin Rabbit IgG pSer601 Log in to see Polyclonal
1 ABIN1955243 DB ELISA Biotin Rabbit IgG pThr400 Log in to see Polyclonal
1 ABIN1955247 DB ELISA WB Biotin Rabbit IgG pThr439 Log in to see Polyclonal
1 ABIN1956227 DB ELISA FITC Rabbit IgG pSer445 Log in to see Polyclonal

General

Antigen V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
Epitope pThr439
(36), (33), (29), (24), (22), (21), (20), (18), (16), (13), (11), (10), (10), (9), (8), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(406), (85), (69), (13), (10), (4), (4), (3), (1), (1), (1), (1)
Host Rabbit
(308), (94), (3), (2)
Clonality (Clone)
Polyclonal   ( )
Conjugate This BRAF antibody is un-conjugated
(20), (20), (20), (17), (17), (17), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Dot Blot (DB)
(268), (210), (145), (77), (71), (45), (39), (22), (11), (8), (5), (4), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-BRAF Antibody

Target Details BRAF Application Details Handling ProductDetails: References for anti-BRAF antibody (ABIN389701) Images
Specificity This Phospho-BRAF-T439 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T439 of human BRAF.
Predicted Reactivity Chicken
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This BRAF Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T439 of human BRAF.
Clone RB10937
Isotype Ig

Target Details BRAF

Product Details anti-BRAF Antibody Application Details Handling ProductDetails: References for anti-BRAF antibody (ABIN389701) Images back to top
Antigen
Alternative Name BRAF (BRAF Antibody Abstract)
Background BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neuron. Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome), also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
Synonyms: B-Raf proto-oncogene serine/threonine-protein kinase, p94, v-Raf murine sarcoma viral oncogene homolog B1, BRAF, BRAF1, RAFB1,
Molecular Weight 84437 DA
Gene ID 673
UniProt P15056
Research Area Apoptosis/Necrosis, Cell Structure, Phospho-specific antibodies, Neurology
Pathways MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy

Application Details

Product Details anti-BRAF Antibody Target Details BRAF Handling ProductDetails: References for anti-BRAF antibody (ABIN389701) Images back to top
Application Notes DB = 1:500
Restrictions For Research Use only

Handling

Product Details anti-BRAF Antibody Target Details BRAF Application Details ProductDetails: References for anti-BRAF antibody (ABIN389701) Images back to top
Format Liquid
Concentration 0.35 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

ProductDetails: References for anti-BRAF antibody (ABIN389701)

Product Details anti-BRAF Antibody Target Details BRAF Application Details Handling Images back to top
Background publications

Frattini, Ferrario, Bressan, Balestra, De Cecco, Mondellini, Bongarzone, Collini, Gariboldi, Pilotti, Pierotti, Greco: "Alternative mutations of BRAF, RET and NTRK1 are associated with similar but distinct gene expression patterns in papillary thyroid cancer." in: Oncogene, Vol. 23, Issue 44, pp. 7436-40, 2004

Tsavachidou, Coleman, Athanasiadis, Li, Licht, Olson, Weber: "SPRY2 is an inhibitor of the ras/extracellular signal-regulated kinase pathway in melanocytes and melanoma cells with wild-type BRAF but not with the V599E mutant." in: Cancer research, Vol. 64, Issue 16, pp. 5556-9, 2004

Images

Product Details anti-BRAF Antibody Target Details BRAF Application Details Handling ProductDetails: References for anti-BRAF antibody (ABIN389701) back to top
Supplier Images
Dot Blot (DB) image for anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) (pThr439) antibody (ABIN389701) Dot blot analysis of phospho-BRAF-T439 polyclonal antibody(ABIN389701) on nitrocellul...