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anti-Human BRAF antibody for Dot Blot

Recommended BRAF Antibody (supplied by: Log in to see )

Antigen
V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
  • B-Raf
  • braf1
  • rafb1
  • b-raf1
  • sierrabraf
  • BRAF
  • 9930012E13Rik
  • AA120551
  • AA387315
  • AA473386
  • B-raf
  • Braf-2
  • Braf2
  • C230098H17
  • C87398
  • D6Ertd631e
  • Braf
  • HYPT11
  • SME
  • Sm-E
  • B-RAF1
  • BRAF1
  • NS7
  • RAFB1
  • C-RMIL
  • RMIL
  • v-raf murine sarcoma viral oncogene homolog B
  • v-raf murine sarcoma viral oncogene homolog B1
  • Braf transforming gene
  • Braf transforming gene, related sequence 1
  • small nuclear ribonucleoprotein polypeptide E
  • braf
  • BRAF
  • LOC100219601
  • Braf
  • Braf-rs1
  • SNRPE
Epitope
pThr400
29
26
24
23
22
19
18
17
16
16
15
13
9
9
7
6
5
4
4
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
400
58
46
6
4
1
1
1
Host
Rabbit
304
93
3
2
Clonality
Polyclonal
Conjugate
This BRAF antibody is un-conjugated
20
20
20
17
17
17
3
3
3
3
3
3
3
3
3
Application
Dot Blot (DB)
258
214
148
81
53
44
33
16
11
5
4
2
1
1
1
Options
Supplier
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Catalog No. ABIN389897
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
27.435493 ABIN389701 DB Rabbit Ig pThr439 Log in to see Polyclonal 2
27.435493 ABIN389791 DB Rabbit Ig pThr598 Log in to see Polyclonal
27.435493 ABIN389803 DB Rabbit Ig pSer445 Log in to see Polyclonal
27.435493 ABIN2159586 DB Rabbit Ig Fraction pThr400 Log in to see Polyclonal
27.435493 ABIN2159585 DB Rabbit Ig Fraction pThr439 Log in to see Polyclonal
27.435493 ABIN3029547 DB ELISA Rabbit Ig Fraction pSer445 Log in to see Polyclonal
27.435493 ABIN3029548 DB ELISA Rabbit Ig Fraction pThr400 Log in to see Polyclonal
27.435493 ABIN3029549 DB ELISA Rabbit Ig Fraction pThr439 Log in to see Polyclonal
27.435493 ABIN2157912 DB ELISA Rabbit Ig Fraction Log in to see Polyclonal
27.435493 ABIN2157913 DB ELISA Rabbit Ig Fraction Log in to see Polyclonal
1 ABIN1958776 DB ELISA PE Rabbit IgG Thr598 Log in to see Polyclonal
1 ABIN1952913 DB ELISA Alkaline Phosphatase (AP) Rabbit IgG Ser445 Log in to see Polyclonal
1 ABIN1952951 DB ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Ser601 Log in to see Polyclonal
1 ABIN1955243 DB ELISA Biotin Rabbit IgG Thr400 Log in to see Polyclonal
1 ABIN1955247 DB ELISA WB Biotin Rabbit IgG Thr439 Log in to see Polyclonal
1 ABIN1955062 DB ELISA Biotin Rabbit IgG Ser445 Log in to see Polyclonal
1 ABIN1955095 DB ELISA WB Biotin Rabbit IgG Ser601 Log in to see Polyclonal
1 ABIN1958558 DB ELISA PE Rabbit IgG Ser445 Log in to see Polyclonal
1 ABIN1958596 DB ELISA WB PE Rabbit IgG Ser601 Log in to see Polyclonal
1 ABIN1958755 DB ELISA PE Rabbit IgG Thr400 Log in to see Polyclonal

Top referenced anti-BRAF antibody for Dot Blot

  • Human Polyclonal BRAF Primary Antibody for DB - ABIN389897 (3 Publications): Frattini, Ferrario, Bressan, Balestra, De Cecco, Mondellini, Bongarzone, Collini, Gariboldi, Pilotti, Pierotti, Greco: Alternative mutations of BRAF, RET and NTRK1 are associated with similar but distinct gene expression patterns in papillary thyroid cancer. in Oncogene 2004 (PubMed)

Similar anti-BRAF Antibodies

Application / Reactivity Human
Dot Blot (DB) 82 Antibodies
ELISA 212 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 11 Antibodies
Flow Cytometry (FACS) 16 Antibodies
Functional Studies (Func) 1 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 42 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies
Immunohistochemistry (IHC) 148 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 53 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Luminex Assay (LMNX) 4 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 256 Antibodies

General

Antigen V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
Epitope pThr400
(29), (26), (24), (23), (22), (19), (18), (17), (16), (16), (15), (13), (9), (9), (7), (6), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(400), (58), (46), (6), (4), (1), (1), (1)
Host Rabbit
(304), (93), (3), (2)
Clonality (Clone)
Polyclonal   ( )
Conjugate This BRAF antibody is un-conjugated
(20), (20), (20), (17), (17), (17), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Dot Blot (DB)
(258), (214), (148), (81), (53), (44), (33), (16), (11), (5), (4), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-BRAF Antibody

Target Details BRAF Application Details Handling References for anti-BRAF Antibody (ABIN389897) Images
Specificity This Phospho-BRAF-pT400 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T400 of human BRAF.
Predicted Reactivity Mouse (Murine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This BRAF Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T400 of human BRAF.
Clone RB13721
Isotype Ig

Target Details BRAF

Product Details anti-BRAF Antibody Application Details Handling References for anti-BRAF Antibody (ABIN389897) Images back to top
Antigen
Alternative Name BRAF (BRAF Antibody Abstract)
Background BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome), and a wide range of cancers such as lung cancer, non-Hodgkins lymphoma, and colorectal cancer.
Synonyms: B-Raf proto-oncogene serine/threonine-protein kinase, p94, v-Raf murine sarcoma viral oncogene homolog B1
Molecular Weight 84437 DA
Gene ID 673
UniProt P15056
Research Area Cell Structure, Phospho-specific antibodies
Pathways MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-BRAF Antibody Target Details BRAF Handling References for anti-BRAF Antibody (ABIN389897) Images back to top
Application Notes DB = 1:500
Restrictions For Research Use only

Handling

Product Details anti-BRAF Antibody Target Details BRAF Application Details References for anti-BRAF Antibody (ABIN389897) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-BRAF Antibody (ABIN389897)

Product Details anti-BRAF Antibody Target Details BRAF Application Details Handling Images back to top
Product cited in:

Duan, Cobb: "Calcineurin increases glucose activation of ERK1/2 by reversing negative feedback." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 51, pp. 22314-9, 2010

Background publications

Frattini, Ferrario, Bressan, Balestra, De Cecco, Mondellini, Bongarzone, Collini, Gariboldi, Pilotti, Pierotti, Greco: "Alternative mutations of BRAF, RET and NTRK1 are associated with similar but distinct gene expression patterns in papillary thyroid cancer." in: Oncogene, Vol. 23, Issue 44, pp. 7436-40, 2004

Tsavachidou, Coleman, Athanasiadis, Li, Licht, Olson, Weber: "SPRY2 is an inhibitor of the ras/extracellular signal-regulated kinase pathway in melanocytes and melanoma cells with wild-type BRAF but not with the V599E mutant." in: Cancer research, Vol. 64, Issue 16, pp. 5556-9, 2004

Images

Product Details anti-BRAF Antibody Target Details BRAF Application Details Handling References for anti-BRAF Antibody (ABIN389897) back to top
Supplier Images
Dot Blot (DB) image for anti-BRAF antibody (V-Raf Murine Sarcoma Viral Oncogene Homolog B1) (pThr400) (ABIN389897) Dot blot analysis of anti-BRAF Phospho-specific Pab (ABIN389897) on nitrocellulose me...