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|Application / Reactivity||Human|
|Dot Blot (DB)||83 Antibodies|
|ELISA (Capture)||1 Antibodies|
|Flow Cytometry (FACS)||17 Antibodies|
|Functional Studies (Func)||1 Antibodies|
|Immunocytochemistry (ICC)||6 Antibodies|
|Immunofluorescence (IF)||42 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||33 Antibodies|
|Immunohistochemistry (IHC)||152 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||45 Antibodies|
|Immunoprecipitation (IP)||4 Antibodies|
|Luminex Assay (LMNX)||4 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Western Blotting (WB)||250 Antibodies|
|Antigen||V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies|
|Conjugate||This BRAF antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Western Blotting (WB)
|6 references available|
|Supplier||Log in to see|
Product Details anti-BRAF AntibodyTarget Details BRAF Application Details Handling References for anti-BRAF Antibody (ABIN1881118) Images
|Purification||This antibody is purified through a protein A column, followed by peptide affinity purification. Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)|
|Immunogen||This BRAF antibody is generated from rabbits immunized with BRAF recombinant protein.|
Target Details BRAFProduct Details anti-BRAF Antibody Application Details Handling References for anti-BRAF Antibody (ABIN1881118) Images back to top
|Alternative Name||BRAF (BRAF Antibody Abstract)|
BRAF, a member of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. This cytoplasmic protein is expressed in brain and testis. Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL). This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.
Synonyms: BRAF, BRAF1, RAFB1, Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1
|Pathways||MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-BRAF Antibody Target Details BRAF Handling References for anti-BRAF Antibody (ABIN1881118) Images back to top
|Application Notes||WB: 1:1000,FACS: 1:10-50|
|Restrictions||For Research Use only|
HandlingProduct Details anti-BRAF Antibody Target Details BRAF Application Details References for anti-BRAF Antibody (ABIN1881118) Images back to top
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
References for anti-BRAF Antibody (ABIN1881118)Product Details anti-BRAF Antibody Target Details BRAF Application Details Handling Images back to top
|Product cited in:||
Weisbart, Chan, Heinze et al.: "BRAF drives synovial fibroblast transformation in rheumatoid arthritis." in: The Journal of biological chemistry, Vol. 285, Issue 45, pp. 34299-303, 2010 (PubMed).
Lee, Yoo, Soung et al.: "BRAF mutations in non-Hodgkin's lymphoma." in: British journal of cancer, Vol. 89, Issue 10, pp. 1958-60, 2003 (PubMed).
Hingorani, Jacobetz, Robertson et al.: "Suppression of BRAF(V599E) in human melanoma abrogates transformation." in: Cancer research, Vol. 63, Issue 17, pp. 5198-202, 2003 (PubMed).
Naoki, Chen, Richards et al.: "Missense mutations of the BRAF gene in human lung adenocarcinoma." in: Cancer research, Vol. 62, Issue 23, pp. 7001-3, 2002 (PubMed).
Davies, Bignell, Cox et al.: "Mutations of the BRAF gene in human cancer. ..." in: Nature, Vol. 417, Issue 6892, pp. 949-54, 2002 (PubMed).
Stephens, Sithanandam, Copeland et al.: "95-kilodalton B-Raf serine/threonine kinase: identification of the protein and its major autophosphorylation site." in: Molecular and cellular biology, Vol. 12, Issue 9, pp. 3733-42, 1992 (PubMed).
ImagesProduct Details anti-BRAF Antibody Target Details BRAF Application Details Handling References for anti-BRAF Antibody (ABIN1881118) back to top