anti-Human BRAF antibody for Flow Cytometry

Recommended BRAF Antibody (supplied by: Log in to see )

Antigen
V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
  • B-Raf
  • braf1
  • rafb1
  • b-raf1
  • sierrabraf
  • BRAF
  • 9930012E13Rik
  • AA120551
  • AA387315
  • AA473386
  • B-raf
  • Braf-2
  • Braf2
  • C230098H17
  • C87398
  • D6Ertd631e
  • Braf
  • HYPT11
  • SME
  • Sm-E
  • B-RAF1
  • BRAF1
  • NS7
  • RAFB1
  • C-RMIL
  • RMIL
  • v-raf murine sarcoma viral oncogene homolog B
  • v-raf murine sarcoma viral oncogene homolog B1
  • Braf transforming gene
  • Braf transforming gene, related sequence 1
  • small nuclear ribonucleoprotein polypeptide E
  • braf
  • BRAF
  • LOC100219601
  • Braf
  • Braf-rs1
  • SNRPE
Reactivity
Human
403
85
69
13
10
4
4
3
1
1
1
1
Host
Rabbit
305
94
3
2
Clonality
Polyclonal
Conjugate
This BRAF antibody is un-conjugated
20
20
20
17
17
17
3
3
3
3
3
3
3
3
3
3
3
Application
Flow Cytometry (FACS), Western Blotting (WB)
264
210
143
78
70
44
39
21
10
8
4
4
1
1
1
1
Options
Supplier
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Catalog No. ABIN1881118
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.342041 ABIN2452815 FACS IF WB Mouse IgG1 Log in to see 1D2
13.342041 ABIN3029542 FACS IF IHC ELISA WB Rabbit Ig Fraction AA 424-453 Log in to see Polyclonal
13.342041 ABIN3029544 FACS ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
1 ABIN1496953 FACS IF WB Mouse IgG1 Log in to see 1D2
1 ABIN1496952 FACS IF IHC (p) WB Mouse IgG1 Log in to see 1D4
1 ABIN2452440 FACS IF IHC WB Mouse IgG1 Log in to see 5A9
1 ABIN2452816 FACS IF IHC WB Mouse IgG1 Log in to see 1E2
1 ABIN2452814 FACS IF IHC WB Mouse IgG1 Log in to see 1D4
1 ABIN1496951 FACS IF IHC (p) WB Mouse IgG1 Log in to see 5A9
1 ABIN1956264 DB ELISA FACS IHC IP WB FITC Rabbit IgG Ser601 Log in to see Polyclonal
1 ABIN1958596 DB ELISA FACS IHC IP WB PE Rabbit IgG Ser601 Log in to see Polyclonal
1 ABIN1955095 DB ELISA FACS IHC IP WB Biotin Rabbit IgG Ser601 Log in to see Polyclonal
1 ABIN1952951 DB ELISA FACS IHC IP WB Alkaline Phosphatase (AP) Rabbit IgG Ser601 Log in to see Polyclonal
1 ABIN1953823 DB ELISA FACS IHC IP WB APC Rabbit IgG Ser601 Log in to see Polyclonal
1 ABIN1957472 DB ELISA FACS IHC IP WB HRP Rabbit IgG Ser601 Log in to see Polyclonal
1 ABIN1840333 FACS IF IHC (p) WB Rabbit Ser445 Log in to see Polyclonal
1 ABIN1840331 FACS IF IHC (p) WB Rabbit AA 424-453 Log in to see Polyclonal
1 ABIN2134876 FACS IF IHC WB Mouse IgG1 Log in to see 1D4
1 ABIN2134866 FACS IF IHC WB Mouse IgG1 Log in to see 5A9
1 ABIN2134875 FACS IF WB Mouse IgG1 Log in to see 1D2

Top referenced anti-BRAF antibody for Flow Cytometry

Similar anti-BRAF Antibodies

Application / Reactivity Human
Dot Blot (DB) 78 Antibodies
ELISA 209 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 10 Antibodies
Flow Cytometry (FACS) 22 Antibodies
Functional Studies (Func) 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 43 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 39 Antibodies
Immunohistochemistry (IHC) 143 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 70 Antibodies
Immunoprecipitation (IP) 8 Antibodies
Luminex Assay (LMNX) 4 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 264 Antibodies

General

Antigen V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
Reactivity Human
(403), (85), (69), (13), (10), (4), (4), (3), (1), (1), (1), (1)
Host Rabbit
(305), (94), (3), (2)
Clonality (Clone)
Polyclonal   ( )
Conjugate This BRAF antibody is un-conjugated
(20), (20), (20), (17), (17), (17), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Flow Cytometry (FACS), Western Blotting (WB)
(264), (210), (143), (78), (70), (44), (39), (21), (10), (8), (4), (4), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-BRAF Antibody

Target Details BRAF Application Details Handling ProductDetails: References for anti-BRAF antibody (ABIN1881118) Images
Purification This antibody is purified through a protein A column, followed by peptide affinity purification. Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Immunogen This BRAF antibody is generated from rabbits immunized with BRAF recombinant protein.
Clone RB22681

Target Details BRAF

Product Details anti-BRAF Antibody Application Details Handling ProductDetails: References for anti-BRAF antibody (ABIN1881118) Images back to top
Antigen
Alternative Name BRAF (BRAF Antibody Abstract)
Background BRAF, a member of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. This cytoplasmic protein is expressed in brain and testis. Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL). This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.

Synonyms: BRAF, BRAF1, RAFB1, Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1
NCBI Accession NP_004324
UniProt P15056
Pathways MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy

Application Details

Product Details anti-BRAF Antibody Target Details BRAF Handling ProductDetails: References for anti-BRAF antibody (ABIN1881118) Images back to top
Application Notes WB: 1:1000,FACS: 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-BRAF Antibody Target Details BRAF Application Details ProductDetails: References for anti-BRAF antibody (ABIN1881118) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

ProductDetails: References for anti-BRAF antibody (ABIN1881118)

Product Details anti-BRAF Antibody Target Details BRAF Application Details Handling Images back to top
Product cited in:

Weisbart, Chan, Heinze, Mory, Nishimura, Colburn: "BRAF drives synovial fibroblast transformation in rheumatoid arthritis." in: The Journal of biological chemistry, Vol. 285, Issue 45, pp. 34299-303, 2010

Hingorani, Jacobetz, Robertson, Herlyn, Tuveson: "Suppression of BRAF(V599E) in human melanoma abrogates transformation." in: Cancer research, Vol. 63, Issue 17, pp. 5198-202, 2003

Lee, Yoo, Soung, Kim, Park, Kim, Lee, Park, Cho, Kim, Ko, Kim, Nam, Lee, Lee: "BRAF mutations in non-Hodgkin's lymphoma." in: British journal of cancer, Vol. 89, Issue 10, pp. 1958-60, 2003

Davies, Bignell, Cox, Stephens, Edkins, Clegg, Teague, Woffendin, Garnett, Bottomley, Davis, Dicks, Ewing, Floyd, Gray, Hall, Hawes, Hughes, Kosmidou, Menzies, Mould, Parker, Stevens, Watt, Hooper et al.: "Mutations of the BRAF gene in human cancer. ..." in: Nature, Vol. 417, Issue 6892, pp. 949-54, 2002

Naoki, Chen, Richards, Sugarbaker, Meyerson: "Missense mutations of the BRAF gene in human lung adenocarcinoma." in: Cancer research, Vol. 62, Issue 23, pp. 7001-3, 2002

Stephens, Sithanandam, Copeland, Kaplan, Rapp, Morrison: "95-kilodalton B-Raf serine/threonine kinase: identification of the protein and its major autophosphorylation site." in: Molecular and cellular biology, Vol. 12, Issue 9, pp. 3733-42, 1992

Images

Product Details anti-BRAF Antibody Target Details BRAF Application Details Handling ProductDetails: References for anti-BRAF antibody (ABIN1881118) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-BRAF antibody (V-Raf Murine Sarcoma Viral Oncogene Homolog B1) (ABIN1881118) BRAF Antibody (ABIN1881118) flow cytometric analysis of CEM cells (bottom histogram) ...
Western Blotting (WB) image for anti-BRAF antibody (V-Raf Murine Sarcoma Viral Oncogene Homolog B1) (ABIN1881118) BRAF Antibody (ABIN1881118) flow cytometric analysis of CEM cells (bottom histogram) ...