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anti-Human BRAF antibody for Functional Studies

Recommended BRAF Antibody (supplied by: Log in to see )

Antigen
V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
  • B-Raf
  • braf1
  • rafb1
  • b-raf1
  • sierrabraf
  • BRAF
  • 9930012E13Rik
  • AA120551
  • AA387315
  • AA473386
  • B-raf
  • Braf-2
  • Braf2
  • C230098H17
  • C87398
  • D6Ertd631e
  • Braf
  • HYPT11
  • SME
  • Sm-E
  • B-RAF1
  • BRAF1
  • NS7
  • RAFB1
  • C-RMIL
  • RMIL
  • v-raf murine sarcoma viral oncogene homolog B
  • v-raf murine sarcoma viral oncogene homolog B1
  • Braf transforming gene
  • Braf transforming gene, related sequence 1
  • small nuclear ribonucleoprotein polypeptide E
  • braf
  • BRAF
  • LOC100219601
  • Braf
  • Braf-rs1
  • SNRPE
Epitope
AA 10-22
33
26
24
23
22
19
19
18
18
16
16
13
9
9
7
6
5
4
4
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
405
60
48
6
4
1
1
1
Host
Rabbit
310
92
3
2
Clonality
Polyclonal
Conjugate
This BRAF antibody is un-conjugated
20
20
20
17
17
17
3
3
3
3
3
3
3
3
3
3
3
Application
Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
256
213
147
82
52
44
39
16
11
5
4
2
1
1
Supplier
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Catalog No. ABIN2738608
$ 595.83
Plus shipping costs $45.00

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Application / Reactivity Human
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ELISA 211 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 11 Antibodies
Flow Cytometry (FACS) 16 Antibodies
Functional Studies (Func) 1 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 42 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 39 Antibodies
Immunohistochemistry (IHC) 147 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 53 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Luminex Assay (LMNX) 4 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 255 Antibodies

General

Antigen V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
Epitope AA 10-22
(33), (26), (24), (23), (22), (19), (19), (18), (18), (16), (16), (13), (9), (9), (7), (6), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(405), (60), (48), (6), (4), (1), (1), (1)
Host Rabbit
(310), (92), (3), (2)
Clonality
Polyclonal
Conjugate This BRAF antibody is un-conjugated
(20), (20), (20), (17), (17), (17), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(256), (213), (147), (82), (52), (44), (39), (16), (11), (5), (4), (2), (1), (1)
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Product Details anti-BRAF Antibody

Target Details BRAF Application Details Handling Images
Specificity Recognizes B-Raf, Mr 95kD. Species cross-reactivity: mouse and rat.
Purification Immunoaffinity purified
Immunogen KLH-conjugated, synthetic peptide corresponding to aa10-22 (C-GGAEPGQALFNGD) of human B-Raf. The immunizing sequence has 12/13 identical amino acids in mouse. Percent identity by BLAST analysis: Human, Gorilla (100%), Gibbon, Marmoset, Mouse, Rat (92%).
ImmunogenType: Synthetic peptide - KLH conjugated
Isotype IgG

Target Details BRAF

Product Details anti-BRAF Antibody Application Details Handling Images back to top
Antigen
Alternative Name BRAF / B-Raf (BRAF Antibody Abstract)
Background Synonyms: BRAF, 94 kDa B-raf protein, B-raf, BRAF1, B Raf, Proto-oncogene B-Raf, p94, B-RAF1, NS7, ONCOGENE BRAF, RAFB1
NCBI Accession 673
Pathways MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-BRAF Antibody Target Details BRAF Handling Images back to top
Application Notes IHC-P (1:100)
Western Blot (MCF-7 and PC12 cells): 1:250-1:2000. MAP Kinase Activation and MBP Phosphorylation: 20-40 μL Immunoprecipitated active B-Raf from NGF stimulated PC12 cell lysates, as demonstrated in a coupled phosphorylation assay that used the enzyme i ...
Restrictions For Research Use only

Handling

Product Details anti-BRAF Antibody Target Details BRAF Application Details Images back to top
Format Liquid
Buffer PBS, pH 7.6, 0.25M sodium chloride, 0.09 % sodium azide, 30 % glycerol
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C
Storage Comment Long term: -20°C, Short term: +4°C. .