anti-Mouse (Murine) BRAF antibody for ELISA

Recommended BRAF Antibody (supplied by: Log in to see )

Antigen
V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
  • B-Raf
  • braf1
  • rafb1
  • b-raf1
  • sierrabraf
  • BRAF
  • 9930012E13Rik
  • AA120551
  • AA387315
  • AA473386
  • B-raf
  • Braf-2
  • Braf2
  • C230098H17
  • C87398
  • D6Ertd631e
  • Braf
  • HYPT11
  • SME
  • Sm-E
  • B-RAF1
  • BRAF1
  • NS7
  • RAFB1
  • C-RMIL
  • RMIL
  • v-raf murine sarcoma viral oncogene homolog B
  • v-raf murine sarcoma viral oncogene homolog B1
  • Braf transforming gene
  • Braf transforming gene, related sequence 1
  • small nuclear ribonucleoprotein polypeptide E
  • braf
  • BRAF
  • LOC100219601
  • Braf
  • Braf-rs1
  • SNRPE
Reactivity
Human, Mouse (Murine)
408
84
69
13
10
4
4
3
1
1
1
1
Host
Mouse
310
94
3
2
Clonality
Monoclonal
Conjugate
This BRAF antibody is un-conjugated
20
20
20
17
17
17
3
3
3
3
3
3
3
3
3
3
3
Application
ELISA, Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)
267
209
144
78
72
45
39
22
11
8
4
4
1
1
1
1
Options
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Catalog No. ABIN2869395
$ 464.29
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN968991 ICC IHC ELISA WB Mouse IgG1 Log in to see 1H12 2
1 ABIN462545 IF IHC (p) ELISA WB Mouse IgG1 AA 285-401 Log in to see 1H12
1 ABIN2878975 IHC (p) ELISA WB Rabbit IgG AA 411-460 Log in to see Polyclonal
1 ABIN2879057 IF IHC (p) ELISA WB Rabbit IgG AA 171-220 Log in to see Polyclonal
1 ABIN2888763 ELISA IHC WB Rabbit IgG AA 576-625 Log in to see Polyclonal
1 ABIN1585648 IP IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN1977057 IF IHC (p) ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN2470813 IF IHC ELISA WB Mouse IgG1 Log in to see 1H12
1 ABIN2883536 ELISA WB Rabbit IgG pThr753 Log in to see Polyclonal
1 ABIN2883533 ELISA IHC (p) WB Rabbit IgG pSer446 Log in to see Polyclonal
1 ABIN2962049 IF IHC (p) ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN4889012 ELISA IF WB Rabbit Internal Region Log in to see Polyclonal
1 ABIN2883534 ELISA IHC (p) WB Rabbit IgG pSer602 Log in to see Polyclonal
1 ABIN2883535 ELISA IHC (p) Rabbit IgG pThr599 Log in to see Polyclonal
1 ABIN2884882 IHC ELISA WB Rabbit IgG AA 390-439 Log in to see Polyclonal
1 ABIN574550 ELISA WB Chicken Internal Region Log in to see Polyclonal
1 ABIN2888762 ELISA IHC Rabbit IgG AA 571-620 Log in to see Polyclonal
1 ABIN2885738 IHC ELISA WB Rabbit IgG pSer446 Log in to see Polyclonal
1 ABIN2885737 ELISA WB Rabbit IgG pThr753 Log in to see Polyclonal
1 ABIN4901047 ELISA WB Mouse IgG2a Log in to see 4E1-E1-F9

General

Antigen V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies
Reactivity Human, Mouse (Murine)
(408), (84), (69), (13), (10), (4), (4), (3), (1), (1), (1), (1)
Host Mouse
(310), (94), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BRAF antibody is un-conjugated
(20), (20), (20), (17), (17), (17), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application ELISA, Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)
(267), (209), (144), (78), (72), (45), (39), (22), (11), (8), (4), (4), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-BRAF Antibody

Target Details BRAF Application Details Handling References for anti-BRAF antibody (ABIN2869395) Images
Purpose Purified Mouse Monoclonal Antibody
Characteristics Synonyms: Serine/threonine-protein kinase B-raf, 2.7.11.1, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1, BRAF, BRAF1, RAFB1
Immunogen Purified recombinant fragment of human BRAF expressed in E. Coli.

Antigen Type: Recombinant Protein
Clone 1H12
Isotype IgG1

Target Details BRAF

Product Details anti-BRAF Antibody Application Details Handling References for anti-BRAF antibody (ABIN2869395) Images back to top
Antigen
Alternative Name BRAF (BRAF Antibody Abstract)
Background BRAF: v-raf murine sarcoma viral oncogene homolog B1, also known as BRAF1, RAFB1, B-RAF1, FLJ95109. Entrez Protein NP_004324. It is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428, and Thr439). B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK), it has a long amino-terminal region,the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highestlevels in the cerebrum and testes and defects in braf are involved in a wide range of cancers. The BRAF gene mutation is frequently detected in papillary thyroid carcinoma,melanocytic nevi, primary cutaneous melanomas and colorectal cancers.
Molecular Weight 84437 Da
Gene ID 673
NCBI Accession NP_004324
UniProt P15056
Pathways MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy

Application Details

Product Details anti-BRAF Antibody Target Details BRAF Handling References for anti-BRAF antibody (ABIN2869395) Images back to top
Application Notes The suggested dilution is: Western Bloting: = 1:500 - 2000. Immunohistochemistry: = 1:200 - 1000. Immunofluorescence: = 1:200 - 1000.
Restrictions For Research Use only

Handling

Product Details anti-BRAF Antibody Target Details BRAF Application Details References for anti-BRAF antibody (ABIN2869395) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-BRAF antibody (ABIN2869395)

Product Details anti-BRAF Antibody Target Details BRAF Application Details Handling Images back to top
Background publications

Di Nicolantonio, Martini, Molinari, Sartore-Bianchi, Arena, Saletti, De Dosso, Mazzucchelli, Frattini, Siena, Bardelli: "Wild-type BRAF is required for response to panitumumab or cetuximab in metastatic colorectal cancer." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 26, Issue 35, pp. 5705-12, 2008

Riesco-Eizaguirre, Gutiérrez-Martínez, García-Cabezas, Nistal, Santisteban: "The oncogene BRAF V600E is associated with a high risk of recurrence and less differentiated papillary thyroid carcinoma due to the impairment of Na+/I- targeting to the membrane." in: Endocrine-related cancer, Vol. 13, Issue 1, pp. 257-69, 2006