|Application / Reactivity||Rat (Rattus)||Mouse (Murine)||Human|
|ELISA||17 Antibodies||26 Antibodies||211 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies||1 Antibodies|
|Immunofluorescence (IF)||2 Antibodies||7 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies||11 Antibodies|
|Immunohistochemistry (IHC)||18 Antibodies||26 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||5 Antibodies||5 Antibodies|
|Immunoprecipitation (IP)||2 Antibodies||2 Antibodies|
|Western Blotting (WB)||30 Antibodies||38 Antibodies|
|Immunocytochemistry (ICC)||2 Antibodies|
|Dot Blot (DB)||83 Antibodies|
|ELISA (Capture)||1 Antibodies|
|Antigen||V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This BRAF antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-BRAF AntibodyTarget Details BRAF Application Details Handling Images
|Immunogen||A synthetic peptide corresponding to residues in human B-Raf was used as immunogen.|
Target Details BRAFProduct Details anti-BRAF Antibody Application Details Handling Images back to top
|Alternative Name||B-Raf proto-oncogene (BRAF Antibody Abstract)|
B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raffamily which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenicsignals from the cell membrane to the nucleus (1). Composed of three conserved region(CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system(2). It has been observed that MAPK is activated by B-Raf in response to nerve growthfactor (3). More than 60% of malignant melanomas were found to contain a specificmutation, B-Raf (V599E) the product of which possesses constitutive kinase activity (4).Mutations in B-Raf have also been identified in lung cancer and non-Hodgkinlymphoma. Alternate Names: anti-94 kDa B raf protein antibody, anti-B raf 1 antibody, anti-B Raf proto oncogeneserine threonine protein kinase antibody, anti-BRAF 1 antibody, anti-Raf antibody, antiBRAF1 antibody, anti-cRmil antibody, antiMGC126806 antibody, anti-MGC138284antibody, anti-Murine sarcoma viral (v-raf) oncogene homolog B1 antibody, anti-Murinesarcoma viral v raf oncogene homolog B1 antibody, anti-p94 antibody, anti-RAFB 1antibody, anti-RAFB1 antibody, anti-v raf murine sarcoma viral oncogene homolog B1antibody.
Gene Symbol: BRAF
|Gene ID||673, 109880, 114486|
|Pathways||MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-BRAF Antibody Target Details BRAF Handling Images back to top
|Application Notes||Recommended dilutions: Flow Cytometry 1:50, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:250-500, Immunoprecipitation 1:50, Western Blot 1:1000-10000|
Protocol specific for B Raf Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
|Restrictions||For Research Use only|
HandlingProduct Details anti-BRAF Antibody Target Details BRAF Application Details Images back to top
|Buffer||PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||-20 °C.|
ImagesProduct Details anti-BRAF Antibody Target Details BRAF Application Details Handling back to top