anti-Human CDT1 antibody for Western Blotting

Recommended CDT1 Antibody (supplied by: Log in to see )

Antigen
Chromatin Licensing and DNA Replication Factor 1 (CDT1) Antibodies
  • CDT1
  • fc37h07
  • im:7158083
  • wu:fc37h07
  • wu:fi38h09
  • xcdt1
  • DUP
  • RIS2
  • 2610318F11Rik
  • AW545653
  • C76791
  • Ris2
  • dup
  • ris2
  • chromatin licensing and DNA replication factor 1
  • CDT1
  • cdt1
  • Cdt1
Epitope
C-Term
35
2
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Yeast
52
11
11
1
1
1
Host
Rabbit
32
18
2
Clonality
Polyclonal
Conjugate
This CDT1 antibody is un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
47
20
17
17
15
15
4
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Catalog No. ABIN2782592
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1424623 ABIN251007 ICC IF IHC IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal 4
3.1424623 ABIN184819 ELISA WB Goat C-Term Log in to see Polyclonal 1
3.1424623 ABIN249645 ELISA WB Goat C-Term Log in to see Polyclonal 1
3.1424623 ABIN2462973 ELISA WB Rabbit Log in to see Polyclonal
3.1424623 ABIN2465519 ELISA WB Goat C-Term Log in to see Polyclonal
3.1424623 ABIN634110 WB Rabbit C-Term Log in to see Polyclonal
3.1424623 ABIN2472916 ELISA WB Goat IgG C-Term Log in to see Polyclonal
3.1424623 ABIN2561792 ELISA WB Goat IgG C-Term Log in to see Polyclonal
3.1424623 ABIN4891821 ICC IF WB Rabbit C-Term Log in to see Polyclonal
1 ABIN341626 WB Rabbit IgG AA 400-449 Log in to see Polyclonal
1 ABIN291971 ELISA WB Goat AA 534-546 Log in to see Polyclonal
1 ABIN2668790 WB Hamster IgG AA 177-557 Log in to see 1
1 ABIN5552803 WB Goat C-Term Log in to see Polyclonal
1 ABIN602907 IP WB Rabbit IgG AA 238-546 Log in to see Polyclonal
1 ABIN2231300 ELISA WB Alkaline Phosphatase (AP) Goat IgG C-Term Log in to see Polyclonal
1 ABIN2231302 ELISA WB Biotin Goat IgG C-Term Log in to see Polyclonal
1 ABIN2231308 IP WB Rabbit IgG AA 238-546, C-Term Log in to see Polyclonal
1 ABIN2231301 ELISA WB APC Goat IgG C-Term Log in to see Polyclonal
1 ABIN2231303 ELISA WB FITC Goat IgG C-Term Log in to see Polyclonal
1 ABIN2231305 ELISA WB PE Goat IgG C-Term Log in to see Polyclonal

General

Antigen Chromatin Licensing and DNA Replication Factor 1 (CDT1) Antibodies
Epitope C-Term
(35), (2), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Yeast
(52), (11), (11), (1), (1), (1)
Host Rabbit
(32), (18), (2)
Clonality
Polyclonal
Conjugate This CDT1 antibody is un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(47), (20), (17), (17), (15), (15), (4)
Pubmed 1 reference available
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Product Details anti-CDT1 Antibody

Target Details CDT1 Application Details Handling References for anti-CDT1 antibody (ABIN2782592) Images
Blocking Peptide Blocking peptide for this product available: ABIN974796
Sequence PATPPATPPA ASPSALKGVS QDLLERIRAK EAQKQLAQMT RCPEQEQRLQ
Predicted Reactivity Cow: 93%, Dog: 77%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 100%, Yeast: 85%
Characteristics This is a rabbit polyclonal antibody against CDT1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human CDT1

Target Details CDT1

Product Details anti-CDT1 Antibody Application Details Handling References for anti-CDT1 antibody (ABIN2782592) Images back to top
Antigen
Alternative Name CDT1 (CDT1 Antibody Abstract)
Background CDT1 cooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. It binds DNA in a sequence-, strand-, and conformation-independent manner and is a potential oncogene.
Alias Symbols: DUP, RIS2
Protein Interaction Partner: FBXO31, SKP1, RBX1, CUL1, DTL, PCNA, HECW2, CDK2, CDC6, CDC5L, ORC2, ORC1, GMNN, DGCR8, UBC, SKP2, CDK1, CCNB1, CCNA2, FOXO3, DDB1, COPS5, CUL4A, CUL4B, SMARCA5, MCM6, MAPK8, MAPK1, HDAC11, KAT7, CCNA1, DDB2, CDK4, CCNE1, TP63, TP73, EP300, KAT2B, CDT1, U
Protein Size: 546
Molecular Weight 60 kDa
Gene ID 81620
NCBI Accession NM_030928, NP_112190
UniProt Q9H211
Research Area Chromatin and Nuclear Signaling, Transcription Factors, DNA/RNA
Pathways MAPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA

Application Details

Product Details anti-CDT1 Antibody Target Details CDT1 Handling References for anti-CDT1 antibody (ABIN2782592) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 546 AA

Restrictions For Research Use only

Handling

Product Details anti-CDT1 Antibody Target Details CDT1 Application Details References for anti-CDT1 antibody (ABIN2782592) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-CDT1 antibody (ABIN2782592)

Product Details anti-CDT1 Antibody Target Details CDT1 Application Details Handling Images back to top
Background publications

Hu, Xiong: "An evolutionarily conserved function of proliferating cell nuclear antigen for Cdt1 degradation by the Cul4-Ddb1 ubiquitin ligase in response to DNA damage." in: The Journal of biological chemistry, Vol. 281, Issue 7, pp. 3753-6, 2006

Images

Product Details anti-CDT1 Antibody Target Details CDT1 Application Details Handling References for anti-CDT1 antibody (ABIN2782592) back to top
Supplier Images
Western Blotting (WB) image for anti-Chromatin Licensing and DNA Replication Factor 1 (CDT1) (C-Term) antibody (ABIN2782592) WB Suggested Anti-CDT1 Antibody Titration: 0.2-1 ug/ml
Positive Control:...
Western Blotting (WB) image for anti-Chromatin Licensing and DNA Replication Factor 1 (CDT1) (C-Term) antibody (ABIN2782592) Sample Type: 3. rat brain extract (80ug)
Primary Antibody Dilution:...