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anti-Rat (Rattus) EIF4EBP1 antibody for ELISA

Recommended EIF4EBP1 Antibody (supplied by: Log in to see )

Antigen
Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) Antibodies
  • 4e-bp
  • 4E-BP
  • 4e-bp1
  • 4E-BP1
  • 4ebp
  • 4eBP
  • 4EBP
  • 4EBP1
  • 43-BP
  • 153432_at
  • AA959816
  • anon-WO0172774.158
  • BcDNA.HL08053
  • BcDNA:HL08053
  • BP-1
  • CG8846
  • CT8705
  • d4e-bp
  • d4E-BP
  • d4E-BP1
  • d4EBP
  • d4eBP
  • Dm 4E-BP
  • Dmel\\CG8846
  • dthor
  • eIF4E-BP
  • EIF4EBP1
  • fc04h09
  • l(2)06270
  • l(2)k07736
  • l(2)k13517
  • PHAS-I
  • Phas1
  • pp20
  • thor
  • Thor/EIF4EBP
  • wu:fc04h09
  • zgc:64137
Epitope
pThr69
59
30
29
22
20
20
17
14
13
13
13
13
12
12
11
10
10
8
8
6
5
5
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine), Rat (Rattus)
436
237
189
10
8
5
2
2
1
1
1
Host
Rabbit
383
91
9
Clonality
Polyclonal
Conjugate
This EIF4EBP1 antibody is un-conjugated
20
17
12
11
8
8
5
5
5
5
5
5
5
5
5
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
313
199
191
87
56
26
21
18
14
7
5
4
4
3
3
1
Supplier
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Catalog No. ABIN1531251
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0012101001 ABIN1531763 IHC ELISA WB Rabbit IgG pThr36 Log in to see Polyclonal
0.0012101001 ABIN1531764 IHC ELISA WB Rabbit IgG pThr45 Log in to see Polyclonal
0.0012101001 ABIN1531250 IHC ELISA WB Rabbit IgG pSer64 Log in to see Polyclonal
0.0012101001 ABIN1532268 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012101001 ABIN1532584 IF IHC ELISA Rabbit IgG Log in to see Polyclonal
0.0012101001 ABIN1532792 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012101001 ABIN1532793 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012101001 ABIN1532269 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012101001 ABIN2162273 IHC ELISA WB Rabbit IgG Ser65 Log in to see Polyclonal
0.0012101001 ABIN2164536 IHC ELISA WB Rabbit IgG Thr36 Log in to see Polyclonal
0.0012101001 ABIN2164538 IHC ELISA WB Rabbit IgG Thr45 Log in to see Polyclonal
0.0012101001 ABIN2164537 IHC ELISA WB Rabbit IgG Thr45 Log in to see Polyclonal
0.0012101001 ABIN2164535 IHC ELISA WB Rabbit IgG Ser64 Log in to see Polyclonal
0.0012101001 ABIN2164540 IHC ELISA WB Rabbit IgG Ser65 Log in to see Polyclonal
0.0012101001 ABIN2164539 IHC ELISA WB Rabbit IgG Thr69 Log in to see Polyclonal
0.0012101001 ABIN2164541 IHC ELISA WB Rabbit IgG Thr70 Log in to see Polyclonal
0.0012101001 ABIN2164534 IF IHC ELISA Rabbit IgG Log in to see Polyclonal
0.0012101001 ABIN1733955 IHC (p) ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0012101001 ABIN1078007 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 2-117 Log in to see Polyclonal
0.0012101001 ABIN3183093 ELISA IHC WB Rabbit IgG Ser33 Log in to see Polyclonal

Similar anti-EIF4EBP1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 46 Antibodies 57 Antibodies
Gel Shift (GS) 2 Antibodies 2 Antibodies
Immunochromatography (IC) 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 12 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 45 Antibodies
Immunohistochemistry (IHC) 101 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 37 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Western Blotting (WB) 120 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 12 Antibodies

General

Antigen Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) Antibodies
Epitope pThr69
(59), (30), (29), (22), (20), (20), (17), (14), (13), (13), (13), (13), (12), (12), (11), (10), (10), (8), (8), (6), (5), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine), Rat (Rattus)
(436), (237), (189), (10), (8), (5), (2), (2), (1), (1), (1)
Host Rabbit
(383), (91), (9)
Clonality
Polyclonal
Conjugate This EIF4EBP1 antibody is un-conjugated
(20), (17), (12), (11), (8), (8), (5), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(313), (199), (191), (87), (56), (26), (21), (18), (14), (7), (5), (4), (4), (3), (3), (1)
Pubmed 1 reference available
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Product Details anti-EIF4EBP1 Antibody

Target Details EIF4EBP1 Application Details Handling References for anti-EIF4EBP1 Antibody (ABIN1531251) Images
Specificity 4E-BP1 (Phospho-Thr69) Antibody detects endogenous levels of 4E- BP1 only when phosphorylated at Thr69.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from mouse 4E-BP1 around the phosphorylation site of Thr69.
Isotype IgG

Target Details EIF4EBP1

Product Details anti-EIF4EBP1 Antibody Application Details Handling References for anti-EIF4EBP1 Antibody (ABIN1531251) Images back to top
Antigen
Alternative Name 4E-BP1 (EIF4EBP1 Antibody Abstract)
Background Molecular weight of target protein: 12 kDa
Synonyms: 4EBP1, EIF4EBP1, Eukaryotic translation initiation factor 4E binding protein 1, Insulin-stimulated EIF-4E binding protein PHAS-I, P/OKCL.6, PHAS-1, PHAS-I
Molecular Weight 12 kDa
Gene ID 13685
UniProt Q60876
Pathways MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling

Application Details

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Handling References for anti-EIF4EBP1 Antibody (ABIN1531251) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:5000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Mm.6700 (NCBI Gene Symbol: EIF4EBP1)

Restrictions For Research Use only

Handling

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details References for anti-EIF4EBP1 Antibody (ABIN1531251) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-EIF4EBP1 Antibody (ABIN1531251)

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details Handling Images back to top
Background publications

Pause, Belsham, Gingras, Donzé, Lin, Lawrence, Sonenberg: "Insulin-dependent stimulation of protein synthesis by phosphorylation of a regulator of 5'-cap function." in: Nature, Vol. 371, Issue 6500, pp. 762-7, 1994

Images

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details Handling References for anti-EIF4EBP1 Antibody (ABIN1531251) back to top
Supplier Images
Western Blotting (WB) image for anti-EIF4EBP1 antibody (Eukaryotic Translation Initiation Factor 4E Binding Protein 1) (pThr69) (ABIN1531251) Western blot analysis of extracts from Jurkat cells treated with EGF 200ng/ml 30', us...
Immunohistochemistry (IHC) image for anti-EIF4EBP1 antibody (Eukaryotic Translation Initiation Factor 4E Binding Protein 1) (pThr69) (ABIN1531251) Immunohistochemistry analysis of paraffin-embedded human brain carcinoma, using 4E-BP...