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anti-Dog (Canine) MAP2K5 antibody for Immunofluorescence

Recommended MAP2K5 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) Antibodies
  • AI324775
  • AI428457
  • ARABIDOPSIS THALIANA MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5
  • ATMAP2K_ALPHA
  • ATMEK5
  • ATMKK5
  • fb73f02
  • HsT17454
  • MAP2K_A
  • MAP KINASE KINASE 5
  • MAP kinase kinase 5
  • MAPKK5
  • Mapkk5
  • Mek5
  • MEK5
  • PRKMK5
  • Prkmk5
  • wu:fb73f02
  • zgc:172137
Epitope
AA 13-188
32
31
17
17
15
13
13
13
13
6
5
5
4
4
3
2
2
2
2
2
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
153
75
75
10
9
7
6
5
3
3
3
3
2
2
1
Host
Mouse
156
15
Clonality
Monoclonal
Conjugate
This MAP2K5 antibody is un-conjugated
9
9
9
5
5
5
5
5
5
5
5
4
4
4
4
Application
Immunofluorescence (IF), Western Blotting (WB)
111
54
47
29
23
20
4
2
2
1
1
Supplier
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Catalog No. ABIN968273
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Top referenced anti-MAP2K5 antibody for Immunofluorescence

Similar anti-MAP2K5 Antibodies

Application / Reactivity Dog (Canine) Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 11 Antibodies 96 Antibodies 43 Antibodies 45 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 4 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 23 Antibodies 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 29 Antibodies 9 Antibodies 10 Antibodies 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 54 Antibodies 32 Antibodies 32 Antibodies
Immunofluorescence (IF) 22 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) Antibodies
Epitope AA 13-188
(32), (31), (17), (17), (15), (13), (13), (13), (13), (6), (5), (5), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(153), (75), (75), (10), (9), (7), (6), (5), (3), (3), (3), (3), (2), (2), (1)
Host Mouse
(156), (15)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MAP2K5 antibody is un-conjugated
(9), (9), (9), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4)
Application Immunofluorescence (IF), Western Blotting (WB)
(111), (54), (47), (29), (23), (20), (4), (2), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-MAP2K5 Antibody

Target Details MAP2K5 Application Details Handling References for anti-MAP2K5 Antibody (ABIN968273) Images
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human MEK5 aa. 13-188
Clone 21-MEK5
Isotype IgG1

Target Details MAP2K5

Product Details anti-MAP2K5 Antibody Application Details Handling References for anti-MAP2K5 Antibody (ABIN968273) Images back to top
Antigen
Alternative Name MEK5 (MAP2K5 Antibody Abstract)
Background The mitogen-activated protein (MAP) kinase cascade participates in the Ras signal transduction pathway. The MAP kinase cascade consists of MEK kinase (Raf-1 or B-Raf), MAP/ERK kinase (MEK1 or MEK2), and extracellular-regulated protein kinase (ERK1 or ERK2). Raf becomes activated following its interaction with Ras-GTP (activated Ras). Via phosphorylation reactions, Raf activates MEK which, in turn, activates ERK. MEK5, also known as MAP Kinase 5, is a 50 kDa protein that is 40% identical to MEK1 and MEK2. Alternative splicing results in two MEK5 isoforms: alpha (50 kDa) and beta (40 kDa). MEK5beta has been reported to be ubiquitously expressed while MEK5alpha is expressed primarily in the liver and brain. The N-terminal amino acid sequence of MEK5alpha resembles sequences found in actin cytoskeletal proteins, indicating that MEK5alpha may associate with the cytoskeleton. However, the role of MEK5 in the MAP kinase pathway and its substrate remain to be identified. This antibody is routinely tested by western blot analysis.
Synonyms: MAP Kinase-5
Molecular Weight 50 kDa
Research Area Cancer, Kinases/Phosphatases
Pathways MAPK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAP2K5 Antibody Target Details MAP2K5 Handling References for anti-MAP2K5 Antibody (ABIN968273) Images back to top
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-MAP2K5 Antibody Target Details MAP2K5 Application Details References for anti-MAP2K5 Antibody (ABIN968273) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-MAP2K5 Antibody (ABIN968273)

Product Details anti-MAP2K5 Antibody Target Details MAP2K5 Application Details Handling Images back to top
Product cited in:

English, Vanderbilt, Xu, Marcus, Cobb: "Isolation of MEK5 and differential expression of alternatively spliced forms." in: The Journal of biological chemistry, Vol. 270, Issue 48, pp. 28897-902, 1996

Zhou, Bao, Dixon: "Components of a new human protein kinase signal transduction pathway." in: The Journal of biological chemistry, Vol. 270, Issue 21, pp. 12665-9, 1995

Images

Product Details anti-MAP2K5 Antibody Target Details MAP2K5 Application Details Handling References for anti-MAP2K5 Antibody (ABIN968273) back to top
Supplier Images
Western Blotting (WB) image for anti-MAP2K5 antibody (Mitogen-Activated Protein Kinase Kinase 5) (AA 13-188) (ABIN968273) Western blot analysis of MEK5 on a Jurkat cell lysate (Human T-cell leukemia, ATCC TI...
Immunofluorescence (IF) image for anti-MAP2K5 antibody (Mitogen-Activated Protein Kinase Kinase 5) (AA 13-188) (ABIN968273) Immunofluorescence staining on human endothelial cells.
 image for anti-MAP2K5 antibody (Mitogen-Activated Protein Kinase Kinase 5) (AA 13-188) (ABIN968273) anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 13-188) antibody (Image 3)
Western Blotting (WB) image for anti-MAP2K5 antibody (Mitogen-Activated Protein Kinase Kinase 5) (AA 13-188) (ABIN968273) anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 13-188) antibody (Image 4)