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anti-Rat (Rattus) MAP3K3 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended MAP3K3 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 3 (MAP3K3) Antibodies
  • AW548911
  • MAPKKK3
  • MEKK3
  • Mekk3
  • mKIAA4031
Epitope
AA 11-25, N-Term
17
15
10
9
5
3
3
2
2
2
1
1
1
1
1
Reactivity
Mouse (Murine), Rat (Rattus)
39
37
7
3
2
2
1
1
1
1
1
1
1
Host
Rabbit
52
9
Clonality
Polyclonal
Conjugate
This MAP3K3 antibody is un-conjugated
2
2
2
2
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
57
23
9
9
5
1
1
1
Supplier
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Catalog No. ABIN3044008
$ 200.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008985059 ABIN3031851 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal

Similar anti-MAP3K3 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunofluorescence (IF) 3 Antibodies 5 Antibodies
Immunohistochemistry (IHC) 3 Antibodies 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies 8 Antibodies
Western Blotting (WB) 7 Antibodies 38 Antibodies
ELISA 18 Antibodies
Immunocytochemistry (ICC) 1 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 3 (MAP3K3) Antibodies
Epitope AA 11-25, N-Term
(17), (15), (10), (9), (5), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine), Rat (Rattus)
(39), (37), (7), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(52), (9)
Clonality
Polyclonal
Conjugate This MAP3K3 antibody is un-conjugated
(2), (2), (2), (2), (2), (2)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(57), (23), (9), (9), (5), (1), (1), (1)
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Product Details anti-MAP3K3 Antibody

Target Details MAP3K3 Application Details Handling Images
Sequence MKDLVALQMS RRTRL
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Mitogen-activated protein kinase kinase kinase 3(MAP3K3) detection. Tested with WB, IHC-P in Mouse,Rat.
Gene Name: mitogen-activated protein kinase kinase kinase 3
Protein Name: Mitogen-activated protein kinase kinase kinase 3
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of mouse MEKK3(11-25aa MKDLVALQMSRRTRL), different from the related rat sequence by two amino acids.
Isotype IgG

Target Details MAP3K3

Product Details anti-MAP3K3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MAP3K3 (MAP3K3 Antibody Abstract)
Background MAP3K3(Mitogen-activated protein kinase kinase kinase 3), also known as MAP/ERK KINASE KINASE 3, MEKK3 or MAPKKK3, is an enzyme that in humans is encoded by the MAP3K3 gene. MAP3K3 is contiguously distal to LYK5 on chromosome 17(Puffenberger et al., 2007). By yeast 2-hybrid analysis of a mouse T-cell cDNA library, Uhlik et al.(2003) showed that a C-terminal fragment of mouse Osm(CCM2) interacted with Mekk3, which activates p38 in response to sorbitol-induced hyperosmotic conditions. Mekk3 and Osm colocalized in the cytoplasmic compartment of cotransfected cells, and the Mekk3-Osm complex was recruited to Rac1 and cytoskeletal actin-containing membrane ruffles in response to sorbitol treatment. Protein interaction assays showed that Osm interacted directly with the Mekk3 substrate Mkk3(MAP2K3), with actin, and with both GDP- and GTP-loaded Rac1. Uhlik et al.(2003) concluded that the RAC1-OSM-MEKK3-MKK3 complex is required for regulation of p38 activity in response to osmotic shock.
UniProt Q61084
Pathways MAPK Signaling

Application Details

Product Details anti-MAP3K3 Antibody Target Details MAP3K3 Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only

Handling

Product Details anti-MAP3K3 Antibody Target Details MAP3K3 Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C,4 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

Images

Product Details anti-MAP3K3 Antibody Target Details MAP3K3 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-MAP3K3 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 3) (AA 11-25) (ABIN3044008) Anti-MEKK3 antibody, IHC(P) IHC(P): Rat Brain Tissue
Immunohistochemistry (IHC) image for anti-MAP3K3 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 3) (AA 11-25) (ABIN3044008) Anti-MEKK3 antibody, IHC(P) IHC(P): Rat Brain Tissue
Western Blotting (WB) image for anti-MAP3K3 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 3) (AA 11-25) (ABIN3044008) Anti-MEKK3 antibody, Western blotting All lanes: Anti MAP3K3 at 0.5ug/ml Lane 1: Rat...