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anti-Human MAP3K5 antibody for Western Blotting

Recommended MAP3K5 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
  • 7420452D20Rik
  • ASK
  • ASK1
  • MAPKKK5
  • Mekk5
  • mitogen-activated protein kinase kinase kinase 5
  • MUD21.11
  • MUD21_11
  • MEKK5
  • MAP3K5
  • mitogen-activated protein kinase kinase kinase 5
  • Map3k5
  • MAPKKK5
  • MAP3K5
Epitope
pSer966
57
44
36
19
19
18
15
15
15
14
12
10
7
6
5
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
369
168
95
6
1
1
1
1
1
1
Host
Rabbit
350
25
Clonality
Polyclonal
Conjugate
This MAP3K5 antibody is un-conjugated
12
12
11
6
6
6
5
5
5
5
5
5
5
5
5
5
5
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
277
143
120
67
65
39
20
11
10
9
4
3
2
1
1
Options
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Catalog No. ABIN1531773
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0882466 ABIN1532802 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 2
3.0882466 ABIN499345 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal 3
3.0882466 ABIN393594 IF WB Mouse IgG1 lambda AA 1231-1374 Log in to see 2D11 5
3.0882466 ABIN499347 EIA IF WB Rabbit IgG Log in to see Polyclonal 3
3.0882466 ABIN969273 ICC FACS IHC ELISA WB Mouse IgG1 Log in to see 2-00E-04 1
3.0882466 ABIN499349 EIA WB Rabbit IgG N-Term Log in to see Polyclonal 2
3.0882466 ABIN392409 WB Rabbit Ig AA 944-973, Ser966 Log in to see Polyclonal
3.0882466 ABIN1531772 IHC ELISA WB Rabbit IgG pSer83 Log in to see Polyclonal
3.0882466 ABIN1532801 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.0882466 ABIN1108200 EIA IF IHC (p) WB Mouse IgG1 Log in to see 2-00E-004 1
3.0882466 ABIN3181921 ELISA IHC WB Rabbit IgG pSer966 Log in to see Polyclonal
3.0882466 ABIN3181920 ELISA IHC WB Rabbit IgG pSer83 Log in to see Polyclonal
3.0882466 ABIN3183404 ELISA IHC WB Rabbit IgG Ser9 Log in to see Polyclonal
3.0882466 ABIN3183403 ELISA IHC WB Rabbit IgG Ser897 Log in to see Polyclonal
3.0882466 ABIN2705520 IHC WB Rabbit Center Log in to see Polyclonal
3.0882466 ABIN2707341 IHC WB Rabbit N-Term Log in to see Polyclonal
3.0882466 ABIN1870371 IHC WB Rabbit IgG pSer83 Log in to see Polyclonal
3.0882466 ABIN1870370 IHC WB Rabbit IgG pSer966 Log in to see Polyclonal
3.0882466 ABIN1873617 WB Rabbit IgG Log in to see Polyclonal
3.0882466 ABIN658363 IHC (p) WB Rabbit Ig AA 1175-1203, C-Term Log in to see Polyclonal

Top referenced anti-MAP3K5 antibody for Western Blotting

Similar anti-MAP3K5 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 8 Antibodies 32 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 9 Antibodies
Immunofluorescence (IF) 3 Antibodies 12 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 39 Antibodies 52 Antibodies
Immunohistochemistry (IHC) 25 Antibodies 54 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 17 Antibodies 35 Antibodies
Western Blotting (WB) 51 Antibodies 101 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Immunoprecipitation (IP) 1 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
Epitope pSer966
(57), (44), (36), (19), (19), (18), (15), (15), (15), (14), (12), (10), (7), (6), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(369), (168), (95), (6), (1), (1), (1), (1), (1), (1)
Host Rabbit
(350), (25)
Clonality
Polyclonal
Conjugate This MAP3K5 antibody is un-conjugated
(12), (12), (11), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(277), (143), (120), (67), (65), (39), (20), (11), (10), (9), (4), (3), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-MAP3K5 Antibody

Target Details MAP3K5 Application Details Handling References for anti-MAP3K5 Antibody (ABIN1531773) Images
Specificity ASK1 (Phospho-Ser966) Antibody detects endogenous levels of ASK1 only when phosphorylated at Ser966.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human ASK1 around the phosphorylation site of Ser966.
Isotype IgG

Target Details MAP3K5

Product Details anti-MAP3K5 Antibody Application Details Handling References for anti-MAP3K5 Antibody (ABIN1531773) Images back to top
Antigen
Alternative Name ASK1 (MAP3K5 Antibody Abstract)
Background Molecular weight of target protein: 154 kDa
Synonyms: Apoptosis signal-regulating kinase 1, ASK-1, kinase ASK1, M3K5, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, MEK kinase 5, MEKK 5, MEKK5, Mitogen-activated protein kinase kinase kinase 5
Molecular Weight 154 kDa
Gene ID 4217
OMIM 602448
UniProt Q99683
Research Area Cardiovascular, Hypertrophy, Apoptosis/Necrosis
Pathways MAPK Signaling, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Handling References for anti-MAP3K5 Antibody (ABIN1531773) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:20000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.186486 (NCBI Gene Symbol: MAP3K5)

Restrictions For Research Use only

Handling

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details References for anti-MAP3K5 Antibody (ABIN1531773) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-MAP3K5 Antibody (ABIN1531773)

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling Images back to top
Background publications

Mungall, Palmer, Sims, Edwards, Ashurst, Wilming, Jones, Horton, Hunt, Scott, Gilbert, Clamp, Bethel, Milne, Ainscough, Almeida, Ambrose, Andrews, Ashwell, Babbage, Bagguley, Bailey, Banerjee, Barker et al.: "The DNA sequence and analysis of human chromosome 6. ..." in: Nature, Vol. 425, Issue 6960, pp. 805-11, 2003

Ichijo, Nishida, Irie, ten Dijke, Saitoh, Moriguchi, Takagi, Matsumoto, Miyazono, Gotoh: "Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways." in: Science (New York, N.Y.), Vol. 275, Issue 5296, pp. 90-4, 1997

Images

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling References for anti-MAP3K5 Antibody (ABIN1531773) back to top
Supplier Images
Western Blotting (WB) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (pSer966) (ABIN1531773) Western blot analysis of extracts from 293 cells treated with TNF(20ng/ml)+calyculinA...
Immunohistochemistry (IHC) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (pSer966) (ABIN1531773) Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ASK1...