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anti-Mouse (Murine) MAP3K5 antibody for ELISA

Recommended MAP3K5 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
  • 7420452D20Rik
  • ASK
  • ASK1
  • MAPKKK5
  • Mekk5
  • mitogen-activated protein kinase kinase kinase 5
  • MUD21.11
  • MUD21_11
  • MEKK5
  • MAP3K5
  • mitogen-activated protein kinase kinase kinase 5
  • Map3k5
  • MAPKKK5
  • MAP3K5
Epitope
pSer966
57
46
44
22
19
19
15
15
15
15
15
9
8
6
5
4
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
380
147
83
4
1
Host
Rabbit
363
25
Clonality
Polyclonal
Conjugate
This MAP3K5 antibody is un-conjugated
12
12
11
6
6
6
5
5
5
5
5
5
5
5
5
5
5
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
282
153
129
65
57
41
24
11
10
9
4
2
1
1
1
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Catalog No. ABIN1531773
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1532802 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 2
1 ABIN3181921 ELISA IHC WB Rabbit IgG pSer966 Log in to see Polyclonal
1 ABIN3183403 ELISA IHC WB Rabbit IgG Ser897 Log in to see Polyclonal
1 ABIN2162757 IHC ELISA WB Rabbit IgG Ser966 Log in to see Polyclonal
1 ABIN2447953 IHC ELISA WB Rabbit IgG Ser966 Log in to see Polyclonal
1 ABIN4281878 ELISA ICC IF IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal
1 ABIN2155943 ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN2155942 ICC ELISA WB Rabbit C-Term Log in to see Polyclonal
1 ABIN2587034 ELISA Rabbit IgG AA 1174-1203 Log in to see Polyclonal
1 ABIN1736719 IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN1736718 ICC IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN1031241 ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN2625103 ELISA WB Biotin Rabbit IgG AA 693-933 Log in to see Polyclonal
1 ABIN1030199 ICC ELISA WB Rabbit Log in to see Polyclonal
1 ABIN2431625 IHC ELISA Rabbit IgG Log in to see Polyclonal
1 ABIN2587029 ELISA Rabbit IgG AA 693-933 Log in to see Polyclonal
1 ABIN1887086 ICC ELISA WB Rabbit C-Term Log in to see Polyclonal
1 ABIN1887951 ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN2958734 ELISA WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN2958733 ICC IF ELISA WB Rabbit IgG C-Term Log in to see Polyclonal

Similar anti-MAP3K5 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
ELISA 6 Antibodies 23 Antibodies 127 Antibodies
Immunocytochemistry (ICC) 2 Antibodies 10 Antibodies
Immunofluorescence (IF) 3 Antibodies 13 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 39 Antibodies 52 Antibodies
Immunohistochemistry (IHC) 22 Antibodies 45 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies 25 Antibodies
Western Blotting (WB) 42 Antibodies 80 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Immunoprecipitation (IP) 1 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
Epitope pSer966
(57), (46), (44), (22), (19), (19), (15), (15), (15), (15), (15), (9), (8), (6), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(380), (147), (83), (4), (1)
Host Rabbit
(363), (25)
Clonality
Polyclonal
Conjugate This MAP3K5 antibody is un-conjugated
(12), (12), (11), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(282), (153), (129), (65), (57), (41), (24), (11), (10), (9), (4), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-MAP3K5 Antibody

Target Details MAP3K5 Application Details Handling References for anti-MAP3K5 Antibody (ABIN1531773) Images
Specificity ASK1 (Phospho-Ser966) Antibody detects endogenous levels of ASK1 only when phosphorylated at Ser966.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human ASK1 around the phosphorylation site of Ser966.
Isotype IgG

Target Details MAP3K5

Product Details anti-MAP3K5 Antibody Application Details Handling References for anti-MAP3K5 Antibody (ABIN1531773) Images back to top
Antigen
Alternative Name ASK1 (MAP3K5 Antibody Abstract)
Background Molecular weight of target protein: 154 kDa
Synonyms: Apoptosis signal-regulating kinase 1, ASK-1, kinase ASK1, M3K5, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, MEK kinase 5, MEKK 5, MEKK5, Mitogen-activated protein kinase kinase kinase 5
Molecular Weight 154 kDa
Gene ID 4217
OMIM 602448
UniProt Q99683
Research Area Cardiovascular, Hypertrophy, Apoptosis/Necrosis
Pathways MAPK Signaling

Application Details

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Handling References for anti-MAP3K5 Antibody (ABIN1531773) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:20000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.186486 (NCBI Gene Symbol: MAP3K5)

Restrictions For Research Use only

Handling

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details References for anti-MAP3K5 Antibody (ABIN1531773) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-MAP3K5 Antibody (ABIN1531773)

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling Images back to top
Background publications

Mungall, Palmer, Sims, Edwards, Ashurst, Wilming, Jones, Horton, Hunt, Scott, Gilbert, Clamp, Bethel, Milne, Ainscough, Almeida, Ambrose, Andrews, Ashwell, Babbage, Bagguley, Bailey, Banerjee, Barker et al.: "The DNA sequence and analysis of human chromosome 6. ..." in: Nature, Vol. 425, Issue 6960, pp. 805-11, 2003

Ichijo, Nishida, Irie, ten Dijke, Saitoh, Moriguchi, Takagi, Matsumoto, Miyazono, Gotoh: "Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways." in: Science (New York, N.Y.), Vol. 275, Issue 5296, pp. 90-4, 1997

Images

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling References for anti-MAP3K5 Antibody (ABIN1531773) back to top
Supplier Images
Western Blotting (WB) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (pSer966) (ABIN1531773) Western blot analysis of extracts from 293 cells treated with TNF(20ng/ml)+calyculinA...
Immunohistochemistry (IHC) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (pSer966) (ABIN1531773) Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ASK1...