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anti-Mouse (Murine) MAP3K5 antibody for Immunohistochemistry (Frozen Sections)

Recommended MAP3K5 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
  • 7420452D20Rik
  • ASK
  • ASK1
  • MAP3K5
  • MAPKKK5
  • MEKK5
  • Mekk5
  • mitogen-activated protein kinase kinase kinase 5
  • MUD21.11
  • MUD21_11
Epitope
AA 693-933
53
48
43
23
22
21
17
15
13
13
13
9
8
6
4
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
375
139
78
4
1
Host
Rabbit
359
23
Clonality
Polyclonal
Conjugate
This MAP3K5 antibody is un-conjugated
13
13
12
7
7
7
5
5
5
5
5
5
5
5
5
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
287
157
135
58
55
40
22
11
8
7
5
2
1
1
1
Supplier
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Catalog No. ABIN1858085
$ 376.20
Plus shipping costs $45.00

Similar anti-MAP3K5 Antibodies

Application / Reactivity Mouse (Murine)
ELISA 24 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 1 Antibodies
Immunocytochemistry (ICC) 11 Antibodies
Immunofluorescence (IF) 14 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 44 Antibodies
Immunohistochemistry (IHC) 46 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 28 Antibodies
Western Blotting (WB) 81 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) Antibodies
Epitope AA 693-933
(53), (48), (43), (23), (22), (21), (17), (15), (13), (13), (13), (9), (8), (6), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(375), (139), (78), (4), (1)
Host Rabbit
(359), (23)
Clonality
Polyclonal
Conjugate This MAP3K5 antibody is un-conjugated
(13), (13), (12), (7), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(287), (157), (135), (58), (55), (40), (22), (11), (8), (7), (5), (2), (1), (1), (1)
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Product Details anti-MAP3K5 Antibody

Target Details MAP3K5 Application Details Handling Images
Sequence MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LGKGTYGI VYAGRDLSNQ VRIAIKEIPE RDSRYSQPLH EEIALHKHLK HKNIVQYLGS FSENGFIKIF MEQVPGGSLS ALLRSKWGPL KDNEQTIGFY TKQILEGLKY LHDNQIVHRD IKGDNVLINT YSGVLKISDF GTSKRLAGIN PCTETFTGTL QYMAPEIIDK GPRGYGKAAD IWSLGCTIIE MATGKPPFYE LGEPQAAMFK VGMFKVHPEI PESMSAEAKA FILKCFEPDP DKR
Specificity It has been selected for its ability to recognize ASK1 in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen ASK1 (AA 693-933)
Isotype IgG

Target Details MAP3K5

Product Details anti-MAP3K5 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Apoptosis Signal Regulating Kinase 1 (ASK1) (MAP3K5 Antibody Abstract)
Pathways MAPK Signaling

Application Details

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant ASK1 (Leu693~Arg933) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2% SDS, 200 mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

Restrictions For Research Use only

Handling

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-MAP3K5 Antibody Target Details MAP3K5 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (AA 693-933) (ABIN1858085) anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 693-933) antibody