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anti-Human PAK1 antibody for Western Blotting

Recommended PAK1 Antibody (supplied by: Log in to see )

Antigen
P21-Activated Kinase 1 (PAK1) Antibodies
  • AW045634
  • pak
  • PAK-1
  • pak-1
  • Paka
  • pakalpha
  • PAKalpha
  • X-PAK1
  • XPak1
Epitope
AA 401-430, Thr423
55
25
24
17
13
10
10
10
9
9
7
7
4
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
314
149
146
4
4
1
1
Host
Rabbit
284
17
15
1
Clonality
Polyclonal
Conjugate
This PAK1 antibody is un-conjugated
11
11
11
7
7
7
4
4
4
4
4
4
4
4
4
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
220
125
124
54
50
44
22
22
13
7
6
4
1
Supplier
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Catalog No. ABIN392432
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12352661 ABIN1944750 WB Rabbit Ig AA 241-275, Center Log in to see Polyclonal 3
0.12352661 ABIN1686090 WB Rabbit IgG C-Term Log in to see Polyclonal
0.12352661 ABIN562102 IF IHC (p) IP ELISA WB Mouse IgG1 kappa AA 191-280, partial Log in to see 4D1 1
0.12352661 ABIN1531693 IHC ELISA WB Rabbit IgG pSer199 Log in to see Polyclonal
0.12352661 ABIN1531365 IHC ELISA WB Rabbit IgG pThr212 Log in to see Polyclonal
0.12352661 ABIN1532069 IHC ELISA WB Rabbit IgG pSer204 Log in to see Polyclonal
0.12352661 ABIN1532383 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.12352661 ABIN1533100 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.12352661 ABIN2855705 ICC IF IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
0.12352661 ABIN3042666 WB Rabbit IgG AA 1-14, N-Term Log in to see Polyclonal
0.12352661 ABIN393779 IF WB Mouse IgG1 kappa AA 191-280 Log in to see 4D1
0.12352661 ABIN498954 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.12352661 ABIN499018 IHC (p) WB Rabbit Log in to see Polyclonal
0.12352661 ABIN498888 IHC (p) WB Rabbit Log in to see Polyclonal
0.12352661 ABIN2706763 IC IF IHC WB Rabbit Center Log in to see Polyclonal
0.12352661 ABIN2706762 IC IF IHC WB Rabbit pSer212 Log in to see Polyclonal
0.12352661 ABIN2466064 ELISA WB Goat Internal Region Log in to see Polyclonal
0.12352661 ABIN223012 IP WB Rabbit IgG Log in to see Polyclonal
0.12352661 ABIN188561 ELISA WB Goat IgG Internal Region Log in to see Polyclonal
0.12352661 ABIN189638 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG N-Term Log in to see EP795Y

Top referenced anti-PAK1 antibody for Western Blotting

Similar anti-PAK1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 22 Antibodies
ELISA 126 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 1 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 15 Antibodies
Immunodiffusion (ID) 7 Antibodies
Immunofluorescence (IF) 57 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 44 Antibodies
Immunohistochemistry (IHC) 122 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 53 Antibodies
Immunoprecipitation (IP) 23 Antibodies
Western Blotting (WB) 224 Antibodies

General

Antigen P21-Activated Kinase 1 (PAK1) Antibodies
Epitope AA 401-430, Thr423
(55), (25), (24), (17), (13), (10), (10), (10), (9), (9), (7), (7), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(314), (149), (146), (4), (4), (1), (1)
Host Rabbit
(284), (17), (15), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This PAK1 antibody is un-conjugated
(11), (11), (11), (7), (7), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(220), (125), (124), (54), (50), (44), (22), (22), (13), (7), (6), (4), (1)
Pubmed 1 reference available
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Product Details anti-PAK1 Antibody

Target Details PAK1 Application Details Handling References for anti-PAK1 Antibody (ABIN392432) Images
Specificity This PAK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T423 of human PAK1.
Predicted Reactivity Cow (Bovine),Mouse (Murine),Rat (Rattus),Rabbit
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This PAK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 401-430 AA from human PAK1.
Clone RB11382
Isotype Ig

Target Details PAK1

Product Details anti-PAK1 Antibody Application Details Handling References for anti-PAK1 Antibody (ABIN392432) Images back to top
Antigen
Alternative Name PAK1 (PAK1 Antibody Abstract)
Background PAK1, a member of the STE20 subfamily of Ser/Thr protein kinases, acts on a variety of targets. It is likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. The protein interacts tightly with GTP-bound but not GDP-bound CDC42/P21 and RAC1. PAK1 binds to the caspase-cleaved p110 isoform of CDC2L1 and CDC2L2, p110C, but not the full-length proteins. It is a component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and GIT1. The protein is autophosphorylated when activated by CDC42/p21. Structurally, the PAK1 contains 1 CRIB domain.
Synonyms: Serine/threonine-protein kinase PAK 1, p21-activated kinase 1, P65-PAK, Alpha-PAK
Molecular Weight 60661 DA
Gene ID 5058
UniProt Q13153
Research Area Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-PAK1 Antibody Target Details PAK1 Handling References for anti-PAK1 Antibody (ABIN392432) Images back to top
Application Notes WB = 1:1000, IF = 1:10-50, IHC (p) = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-PAK1 Antibody Target Details PAK1 Application Details References for anti-PAK1 Antibody (ABIN392432) Images back to top
Format Liquid
Concentration 0.43 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-PAK1 Antibody (ABIN392432)

Product Details anti-PAK1 Antibody Target Details PAK1 Application Details Handling Images back to top
Background publications

Chen, Yin, Zhu, Yan, Ji, Chen, Cai, Zhang, Zong, Hu, Yuan, Shen, Gu: "The C-terminal kinase domain of the p34cdc2-related PITSLRE protein kinase (p110C) associates with p21-activated kinase 1 and inhibits its activity during anoikis." in: The Journal of biological chemistry, Vol. 278, Issue 22, pp. 20029-36, 2003

Images

Product Details anti-PAK1 Antibody Target Details PAK1 Application Details Handling References for anti-PAK1 Antibody (ABIN392432) back to top
Supplier Images
Western Blotting (WB) image for anti-PAK1 antibody (P21-Activated Kinase 1) (AA 401-430) (ABIN392432) Western blot analysis of PAK1 (arrow) using rabbit polyclonal PAK1 Antibody (T423) (A...
Immunohistochemistry (IHC) image for anti-PAK1 antibody (P21-Activated Kinase 1) (AA 401-430) (ABIN392432) Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PAK1 A...