|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||39 Antibodies||40 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||1 Antibodies|
|Immunochromatography (IC)||3 Antibodies||3 Antibodies|
|Immunocytochemistry (ICC)||10 Antibodies||10 Antibodies|
|Immunofluorescence (IF)||34 Antibodies||34 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||5 Antibodies||5 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||52 Antibodies||52 Antibodies|
|Immunohistochemistry (IHC)||66 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||44 Antibodies|
|Immunoprecipitation (IP)||7 Antibodies|
|Western Blotting (WB)||114 Antibodies|
|Antigen||P21-Activated Kinase 1 (PAK1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This PAK1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-PAK1 AntibodyTarget Details PAK1 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of PAK1 protein. (region surrounding Ile206)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen|
|Purity||> 95 % pure by SDS-PAGE|
Target Details PAK1Product Details anti-PAK1 Antibody Application Details Handling Images back to top
|Alternative Name||PAK1 (PAK1 Antibody Abstract)|
|Background||Pak1 belongs to the Ser/Thr protein kinase family and may function by modifying and partially stabilizing thermolabile DNA polymerases, perhaps during DNA repair. Expression of PAK1 increases specific activity of p53 in DNA-binding assays accompanied by a corresponding increase in transactivation. In vitro protein kinase assays show that GST-Pak1 can autophosphorylate, and can phosphorylate casein as an exogenous substrate. The Pak1 protein autophosphorylates on serine residues and preferentially binds to activated Cdc42p both in vitro and in vivo. This binding is mediated through the p21 binding domain on Pak1p and the effector domain on Cdc42p. Pak1 catalytic domain binds to the same highly conserved region on the regulatory domain that binds Cdc42, a GTPase protein capable of activating Pak1.Synonyms: Alpha-PAK, PAK 1, PAK alpha, PAK-1, Serine/threonine-protein kinase PAK 1, p21-activated kinase 1, p65-PAK|
|Molecular Weight||approx. 60 kDa|
|Research Area||Cancer, Apoptosis/Necrosis, Kinases/Phosphatases|
|Pathways||MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Skeletal Muscle Fiber Development|
Application DetailsProduct Details anti-PAK1 Antibody Target Details PAK1 Handling Images back to top
ELISA: 1: 1000approx. 1: 5000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should bedetermined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PAK1 Antibody Target Details PAK1 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||DO NOT FREEZE!|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|
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