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anti-Mouse (Murine) PAK1 antibody for Enzyme Immunoassay

Recommended PAK1 Antibody (supplied by: Log in to see )

Antigen
P21-Activated Kinase 1 (PAK1) Antibodies
  • AW045634
  • pak
  • PAK-1
  • pak-1
  • Paka
  • pakalpha
  • PAKalpha
  • X-PAK1
  • XPak1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
319
152
148
4
4
1
1
Host
Rabbit
289
17
15
1
Clonality
Polyclonal
Conjugate
This PAK1 antibody is un-conjugated
11
11
11
7
7
7
4
4
4
4
4
4
4
4
4
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
225
127
125
58
53
44
22
22
16
7
5
4
1
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Catalog No. ABIN272054
$ 390.50
Plus shipping costs $45.00

Similar anti-PAK1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 33 Antibodies 33 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Flow Cytometry (FACS) 1 Antibodies 1 Antibodies
Immunochromatography (IC) 3 Antibodies 3 Antibodies
Immunocytochemistry (ICC) 14 Antibodies 14 Antibodies
Immunofluorescence (IF) 39 Antibodies 39 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 44 Antibodies 44 Antibodies
Immunohistochemistry (IHC) 70 Antibodies 71 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 32 Antibodies 32 Antibodies
Immunoprecipitation (IP) 5 Antibodies
Western Blotting (WB) 98 Antibodies

General

Antigen P21-Activated Kinase 1 (PAK1) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(319), (152), (148), (4), (4), (1), (1)
Host Rabbit
(289), (17), (15), (1)
Clonality
Polyclonal
Conjugate This PAK1 antibody is un-conjugated
(11), (11), (11), (7), (7), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(225), (127), (125), (58), (53), (44), (22), (22), (16), (7), (5), (4), (1)
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Product Details anti-PAK1 Antibody

Target Details PAK1 Application Details Handling Images
Specificity This antibody detects endogenous levels of PAK1 protein. (region surrounding Ile206)
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Purity > 95 % pure by SDS-PAGE

Target Details PAK1

Product Details anti-PAK1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PAK1 (PAK1 Antibody Abstract)
Background Pak1 belongs to the Ser/Thr protein kinase family and may function by modifying and partially stabilizing thermolabile DNA polymerases, perhaps during DNA repair. Expression of PAK1 increases specific activity of p53 in DNA-binding assays accompanied by a corresponding increase in transactivation. In vitro protein kinase assays show that GST-Pak1 can autophosphorylate, and can phosphorylate casein as an exogenous substrate. The Pak1 protein autophosphorylates on serine residues and preferentially binds to activated Cdc42p both in vitro and in vivo. This binding is mediated through the p21 binding domain on Pak1p and the effector domain on Cdc42p. Pak1 catalytic domain binds to the same highly conserved region on the regulatory domain that binds Cdc42, a GTPase protein capable of activating Pak1.Synonyms: Alpha-PAK, PAK 1, PAK alpha, PAK-1, Serine/threonine-protein kinase PAK 1, p21-activated kinase 1, p65-PAK
Molecular Weight approx. 60 kDa
Gene ID 5058
NCBI Accession NP_001122092
UniProt Q13153
Research Area Cancer, Apoptosis/Necrosis, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-PAK1 Antibody Target Details PAK1 Handling Images back to top
Application Notes ELISA: 1: 1000approx. 1: 5000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should bedetermined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PAK1 Antibody Target Details PAK1 Application Details Images back to top
Concentration 1,0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice DO NOT FREEZE!
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

Images

Product Details anti-PAK1 Antibody Target Details PAK1 Application Details Handling back to top
Supplier Images
 image for anti-PAK1 antibody (P21-Activated Kinase 1) (ABIN272054) anti-P21-Activated Kinase 1 (PAK1) antibody