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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Chicken||Dog (Canine)|
|Western Blotting (WB)||286 Antibodies||114 Antibodies||102 Antibodies||21 Antibodies||6 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Intracellular Flow Cytometry (ICFC)||1 Antibodies||1 Antibodies|
|Immunoprecipitation (IP)||23 Antibodies||11 Antibodies||11 Antibodies||12 Antibodies||6 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||47 Antibodies||24 Antibodies||24 Antibodies|
|Immunohistochemistry (IHC)||102 Antibodies||43 Antibodies||36 Antibodies||7 Antibodies|
|Antigen||Paxillin (PXN) Antibodies|
|Epitope||AA 1-557 Alternatives|
|Reactivity||Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Paxillin antibody is un-conjugated Alternatives|
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
|5 references available|
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Product Details anti-Paxillin AntibodyTarget Details Paxillin Application Details Handling References for anti-Paxillin Antibody (ABIN967711) Images
|Cross-Reactivity||Human, Dog (Canine), Mouse (Murine), Rat (Rattus)|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Chicken Paxillin aa. 1-557|
Target Details PaxillinProduct Details anti-Paxillin Antibody Application Details Handling References for anti-Paxillin Antibody (ABIN967711) Images back to top
|Alternative Name||Paxillin (PXN Antibody Abstract)|
|Background||A number of cytoskeletal proteins are tyrosine phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts. One of these is the 68 kDa paxillin protein. Paxillin is a cytoskeletal component that localizes to the focal adhesions at the ends of actin stress fibers. It is also present in the focal adhesions of Madin-Darby canine kidney epithelial cells, but is absent from the cell adherens junctions of these cells. Paxillin purified from chicken gizzard migrates as a diffuse band on SDS-PAGE with molecular weight of 65-70 kDa. It binds to the rod domain of vinculin, another focal adhesion protein. It is thought that phosphorylation of paxillin may have a role in that disassembly of focal adhesions and stress fibers during transformation. This antibody is routinely tested by Western blot analysis.|
|Molecular Weight||68 kDa|
|Research Area||Cancer, Extracellular Matrix|
|Pathways||MAPK Signaling, EGFR Signaling Pathway|
Application DetailsProduct Details anti-Paxillin Antibody Target Details Paxillin Handling References for anti-Paxillin Antibody (ABIN967711) Images back to top
Related Products: ABIN968533, ABIN967389
|Restrictions||For Research Use only|
HandlingProduct Details anti-Paxillin Antibody Target Details Paxillin Application Details References for anti-Paxillin Antibody (ABIN967711) Images back to top
|Buffer||Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at -20° C.|
References for anti-Paxillin Antibody (ABIN967711)Product Details anti-Paxillin Antibody Target Details Paxillin Application Details Handling Images back to top
|Product cited in:||
van de Water, Houtepen, Huigsloot et al.: "Suppression of chemically induced apoptosis but not necrosis of renal proximal tubular epithelial (LLC-PK1) cells by focal adhesion kinase (FAK). Role of FAK in maintaining focal adhesion organization after acute renal cell injury." in: The Journal of biological chemistry, Vol. 276, Issue 39, pp. 36183-93, 2001 (PubMed).
Even-Ram, Maoz, Pokroy et al.: "Tumor cell invasion is promoted by activation of protease activated receptor-1 in cooperation with the alpha vbeta 5 integrin." in: The Journal of biological chemistry, Vol. 276, Issue 14, pp. 10952-62, 2001 (PubMed).
Nikolopoulos, Turner: "Actopaxin, a new focal adhesion protein that binds paxillin LD motifs and actin and regulates cell adhesion." in: The Journal of cell biology, Vol. 151, Issue 7, pp. 1435-48, 2001 (PubMed).
Wine, Chapin: "Adhesion and signaling proteins spatiotemporally associated with spermiation in the rat." in: Journal of andrology, Vol. 20, Issue 2, pp. 198-213, 1999 (PubMed).
Glenney, Zokas: "Novel tyrosine kinase substrates from Rous sarcoma virus-transformed cells are present in the membrane skeleton." in: The Journal of cell biology, Vol. 108, Issue 6, pp. 2401-8, 1989 (PubMed).
ImagesProduct Details anti-Paxillin Antibody Target Details Paxillin Application Details Handling References for anti-Paxillin Antibody (ABIN967711) back to top