Paxillin antibody (pTyr88)

Catalog No. ABIN7139085

Product Details anti-Paxillin Antibody

Target: Paxillin (PXN)

Binding Specificity: pTyr88

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Paxillin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Cross-Reactivity: Human

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of tyrosine 88 (P-V-Y(p)-G-S) derived from Human Paxillin.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000, IF:1:100-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for Paxillin

Target: Paxillin (PXN)

Alternative Name: PXN (PXN Products)

Synonyms: AW108311 antibody, AW123232 antibody, Pax antibody, CG18061 antibody, CG18576 antibody, CG31794 antibody, CT40481 antibody, CT42454 antibody, DPaxillin antibody, DPxn antibody, DPxn37 antibody, Dmel\\CG31794 antibody, Dpax antibody, DpaxA antibody, PDLP antibody, dPax antibody, pax antibody, wu:fw71f12 antibody, PXN antibody, LOC100220858 antibody, paxillin antibody, Paxillin antibody, paxillin S homeolog antibody, paxillin a antibody, PXN antibody, Pxn antibody, Pax antibody, pxn.S antibody, pxna antibody, LOC696336 antibody, pxn antibody

Background:

Background: An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions

Turner, C.E. (2000) J. Cell Sci. 113, 4139-4140
Burridge, K. et al. (1992) J. Cell Biol. 119, 893-903.
Bellis, S. L. et al. (1997) Biochem. J. 325, 375-381.

Aliases: FLJ16691 antibody, FLJ23042 antibody, Paired box protein Pax 1 antibody, PAX 1 antibody, PAX1 antibody, PAXI_HUMAN antibody, Paxillin alpha antibody, Paxillin antibody, PXN antibody, PXN protein antibody

UniProt: P49023

Pathways: MAPK Signaling, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling