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anti-Human PPARG antibody for Immunohistochemistry (Frozen Sections)

Recommended PPARG Antibody (supplied by: Log in to see )

Antigen
Peroxisome Proliferator-Activated Receptor gamma (PPARG) Antibodies
  • CIMT1
  • GLM1
  • NR1C3
  • Nr1c3
  • PPAR-GAMMA
  • PPAR-gamma
  • PPAR-gamma2
  • PPARG
  • PPARG1
  • PPARg2
  • PPARG2
  • PPARGAMMA
  • PPARgamma
  • PPAR gamma
  • PPARgamma2
  • xPPAR-gamma
Epitope
AA 157-274
51
23
17
15
13
13
13
11
10
9
8
7
5
5
5
5
5
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
256
111
90
17
14
7
7
5
5
4
4
4
2
2
1
1
1
1
1
Host
Rabbit
248
28
5
4
Clonality
Polyclonal
Conjugate
This PPARG antibody is un-conjugated
18
15
9
3
3
3
3
3
3
3
3
3
2
2
2
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
212
121
50
42
33
17
15
12
10
7
6
6
2
2
1
1
Supplier
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Catalog No. ABIN1860284
$ 347.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088254584 ABIN1173887 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 349-488 Log in to see Polyclonal

Similar anti-PPARG Antibodies

Application / Reactivity Human
Chromatin Immunoprecipitation (ChIP) 2 Antibodies
ELISA 104 Antibodies
Enzyme Immunoassay (EIA) 10 Antibodies
Flow Cytometry (FACS) 15 Antibodies
Gel Shift (GS) 1 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunofluorescence (IF) 17 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies
Immunohistochemistry (IHC) 48 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 38 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Western Blotting (WB) 197 Antibodies

General

Antigen Peroxisome Proliferator-Activated Receptor gamma (PPARG) Antibodies
Epitope AA 157-274
(51), (23), (17), (15), (13), (13), (13), (11), (10), (9), (8), (7), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(256), (111), (90), (17), (14), (7), (7), (5), (5), (4), (4), (4), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(248), (28), (5), (4)
Clonality
Polyclonal
Conjugate This PPARG antibody is un-conjugated
(18), (15), (9), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(212), (121), (50), (42), (33), (17), (15), (12), (10), (7), (6), (6), (2), (2), (1), (1)
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Product Details anti-PPARG Antibody

Target Details PPARG Application Details Handling Images
Sequence MGHHHHHHSG SEF- EGCK GFFRRTIRLK LIYDRCDLNC RIHKKSRNKC QYCRFQKCLA VGMSHNAIRF GRMPQAEKEK LLAEISSDID QLNPESADLR ALAKHLYDSY IKSFPLTKAK ARAILTGKTT DKSP
Specificity It has been selected for its ability to recognize PPARg in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen PPARg (AA 157-274)
Isotype IgG

Target Details PPARG

Product Details anti-PPARG Antibody Application Details Handling Images back to top
Antigen
Alternative Name Peroxisome Proliferator Activated Receptor Gamma (PPARg) (PPARG Antibody Abstract)
Pathways MAPK Signaling, Nuclear Receptor Transcription Pathway

Application Details

Product Details anti-PPARG Antibody Target Details PPARG Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant PPARg (Glu157~Pro274) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-PPARG Antibody Target Details PPARG Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-PPARG Antibody Target Details PPARG Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-PPARG antibody (Peroxisome Proliferator-Activated Receptor gamma) (AA 157-274) (ABIN1860284) anti-Peroxisome Proliferator-Activated Receptor gamma (PPARG) (AA 157-274) antibody