anti-Pig (Porcine) PPARG antibody for Enzyme Immunoassay

Recommended PPARG Antibody (supplied by: Log in to see )

Antigen
Peroxisome Proliferator-Activated Receptor gamma (PPARG) Antibodies
  • PPAR gamma
  • PPARg2
  • PPARG
  • Nr1c3
  • PPAR-gamma
  • PPAR-gamma2
  • PPARgamma
  • PPARgamma2
  • NR1C3
  • CIMT1
  • GLM1
  • PPARG1
  • PPARG2
  • xPPAR-gamma
  • PPAR-GAMMA
  • PPARGAMMA
  • peroxisome proliferator-activated receptor gamma
  • peroxisome proliferator activated receptor gamma
  • PPAR gamma 3
  • PPARG
  • Pparg
  • pparg
  • LOC100008892
Epitope
N-Term
39
25
18
17
16
15
15
11
11
9
7
5
5
5
5
5
5
5
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Pig (Porcine)
274
155
120
31
23
15
12
12
11
10
6
6
5
5
5
5
4
4
3
2
2
2
1
1
1
Host
Rabbit
260
33
5
5
Clonality
Polyclonal
Conjugate
This PPARG antibody is un-conjugated
18
15
9
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
243
131
63
41
39
30
20
14
13
9
8
6
3
3
2
2
Supplier
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Catalog No. ABIN302768
$ 500.50
Plus shipping costs $45.00

Similar anti-PPARG Antibodies

Application / Reactivity Human Mouse (Murine) Dog (Canine) Pig (Porcine)
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies 3 Antibodies 1 Antibodies
Western Blotting (WB) 214 Antibodies 107 Antibodies 27 Antibodies 15 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Immunoprecipitation (IP) 8 Antibodies 10 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 39 Antibodies 24 Antibodies 7 Antibodies 4 Antibodies

General

Antigen Peroxisome Proliferator-Activated Receptor gamma (PPARG) Antibodies
Epitope N-Term
(39), (25), (18), (17), (16), (15), (15), (11), (11), (9), (7), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Pig (Porcine)
(274), (155), (120), (31), (23), (15), (12), (12), (11), (10), (6), (6), (5), (5), (5), (5), (4), (4), (3), (2), (2), (2), (1), (1), (1)
Host Rabbit
(260), (33), (5), (5)
Clonality
Polyclonal
Conjugate This PPARG antibody is un-conjugated
(18), (15), (9), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(243), (131), (63), (41), (39), (30), (20), (14), (13), (9), (8), (6), (3), (3), (2), (2)
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Product Details anti-PPARG Antibody

Target Details PPARG Application Details Handling Images
Specificity This antibody is specific to PPAR gamma.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Pig and Canine (Dog).
Species reactivity (tested):Human.
Purification Immunoaffinity Chromatography
Immunogen Synthetic peptide corresponding to a region near the amino terminus of PPAR gamma 2. Epitope: N-terminus

Target Details PPARG

Product Details anti-PPARG Antibody Application Details Handling Images back to top
Antigen
Alternative Name PPAR-gamma (PPARG Antibody Abstract)
Background Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma. The target genes of PPAR-alpha and PPAR-delta are primarily involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and anti-diabetic thiazolidinediones and affects the expression of genes involved in the storage of fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.Synonyms: NR1C3, PPARG, Peroxisome proliferator-activated receptor gamma
Gene ID 5468
NCBI Accession NP_005028
UniProt P37231
Pathways MAPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-PPARG Antibody Target Details PPARG Handling Images back to top
Application Notes ELISA: 1/3200-1/10000. Western Blot: 1/1000-1/3000. Immunohistochemistry on Paraffin Sections: 2.5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PPARG Antibody Target Details PPARG Application Details Images back to top
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Images

Product Details anti-PPARG Antibody Target Details PPARG Application Details Handling back to top
Supplier Images
 image for anti-PPARG antibody (Peroxisome Proliferator-Activated Receptor gamma) (N-Term) (ABIN302768) anti-Peroxisome Proliferator-Activated Receptor gamma (PPARG) (N-Term) antibody