anti-Human MAP1B antibody for Western Blotting

Recommended MAP1B Antibody (supplied by: Log in to see )

Antigen
Microtubule-Associated Protein 1B (MAP1B) Antibodies
  • FUTSCH
  • MAP5
  • Mtap1b
  • A230055D22
  • AI843217
  • LC1
  • Mtap-5
  • Mtap5
  • microtubule-associated protein 1B
  • MAP1B
  • Map1b
Epitope
C-Term, pThr1265
18
15
15
12
2
1
1
1
Reactivity
Chicken, Fish, Human, Mouse (Murine), Rat (Rattus), Reptile, Xenopus laevis
77
61
54
18
18
17
11
11
2
2
1
1
Host
Rabbit
64
19
Clonality
Polyclonal
Conjugate
This MAP1B antibody is un-conjugated
6
4
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
45
31
28
18
16
15
13
5
5
2
1
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Catalog No. ABIN449248
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9497552 ABIN968910 WB Mouse IgG2a AA 1745-1858 Log in to see 6-MAP1B 2
2.9497552 ABIN968911 WB Mouse IgG2a AA 1745-1858 Log in to see 6-MAP1B 2
2.9497552 ABIN261171 IP WB Rabbit AA 900-950 Log in to see Polyclonal 1
2.9497552 ABIN4332597 WB Rabbit IgG C-Term Log in to see Polyclonal
2.9497552 ABIN1873595 FACS IF IHC WB Rabbit IgG Log in to see Polyclonal
2.9497552 ABIN3017669 WB Rabbit Log in to see Polyclonal
1 ABIN269612 ICC IHC (fro) IF IHC MA WB Mouse IgG1 Log in to see AA6 1
1 ABIN4332594 ICC IF IHC IHC (fro) WB DyLight 488 Rabbit C-Term, pThr1265 Log in to see Polyclonal
1 ABIN4332599 ICC IF IHC IHC (fro) WB DyLight 405 Rabbit C-Term, pThr1265 Log in to see Polyclonal
1 ABIN4332601 ICC IF IHC IHC (fro) WB Alexa Fluor 405 Rabbit C-Term, pThr1265 Log in to see Polyclonal
1 ABIN4332602 ICC IF IHC IHC (fro) WB Alexa Fluor 647 Rabbit C-Term, pThr1265 Log in to see Polyclonal
1 ABIN4332593 ICC IF IHC IHC (fro) WB DyLight 650 Rabbit C-Term, pThr1265 Log in to see Polyclonal
1 ABIN4332595 ICC IF IHC IHC (fro) WB DyLight 550 Rabbit C-Term, pThr1265 Log in to see Polyclonal
1 ABIN4332592 ICC IF IHC IHC (fro) WB Biotin Rabbit C-Term, pThr1265 Log in to see Polyclonal
1 ABIN4332600 ICC IF IHC IHC (fro) WB Alexa Fluor 488 Rabbit C-Term, pThr1265 Log in to see Polyclonal
1 ABIN4332603 ICC IF IHC IHC (fro) WB Alexa Fluor 700 Rabbit C-Term, pThr1265 Log in to see Polyclonal
1 ABIN2882221 IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN4332591 IHC IHC (fro) WB HRP Rabbit C-Term, pThr1265 Log in to see Polyclonal
1 ABIN328145 IHC WB Mouse IgG1 Log in to see
1 ABIN4994422 WB Alexa Fluor 488 Rabbit AA 900-950 Log in to see Polyclonal

General

Antigen Microtubule-Associated Protein 1B (MAP1B) Antibodies
Epitope C-Term, pThr1265
(18), (15), (15), (12), (2), (1), (1), (1)
Reactivity Chicken, Fish, Human, Mouse (Murine), Rat (Rattus), Reptile, Xenopus laevis
(77), (61), (54), (18), (18), (17), (11), (11), (2), (2), (1), (1)
Host Rabbit
(64), (19)
Clonality
Polyclonal
Conjugate This MAP1B antibody is un-conjugated
(6), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(45), (31), (28), (18), (16), (15), (13), (5), (5), (2), (1)
Pubmed 7 references available
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Product Details anti-MAP1B Antibody

Target Details MAP1B Application Details Handling ProductDetails: References for anti-MAP1B antibody (ABIN449248) Images
Specificity Phosphorylated Thr1265 on MAP1B (Thr1261 in rat).
Purification Immunogen affinity purified
Immunogen Phosphopeptide made to a C-terminal portion of rat MAP1B. [Swiss-Prot# P15205]

Target Details MAP1B

Product Details anti-MAP1B Antibody Application Details Handling ProductDetails: References for anti-MAP1B antibody (ABIN449248) Images back to top
Antigen
Alternative Name MAP1B (MAP1B Antibody Abstract)
Background Gene Symbol: MAP1B
Molecular Weight Theoretical MW: 320 kDa
Gene ID 4131
UniProt P15205
Pathways Microtubule Dynamics, Regulation of Cell Size

Application Details

Product Details anti-MAP1B Antibody Target Details MAP1B Handling ProductDetails: References for anti-MAP1B antibody (ABIN449248) Images back to top
Application Notes Western Blot 1:1000-1:3000, Immunohistochemistry 1:1000, Immunocytochemistry/Immunofluorescence 1:200, Immunohistochemistry-Frozen 1:1000This MAP1B [p Thr1265] antibody (also known as SuperBUGS) is useful for Western blot, Immunocytochemistry/Immunofluorescence and Immunohistochemistry on frozen sections. In WB a band can be seen at ~320 kDa. Recommended Blocking Buffer for ICC/IF and IHC: 5 % (v/v) normal horse serum, 5 % (v/v) normal goat serum, 0.2 % (w/v) L-lysine, 0.2 % (v/v) Triton X-100 in PBS pH 7.2 The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-MAP1B Antibody Target Details MAP1B Application Details ProductDetails: References for anti-MAP1B antibody (ABIN449248) Images back to top
Format Liquid
Concentration 0.12 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

ProductDetails: References for anti-MAP1B antibody (ABIN449248)

Product Details anti-MAP1B Antibody Target Details MAP1B Application Details Handling Images back to top
Product cited in:

Ketschek, Jones, Spillane, Korobova, Svitkina, Gallo: "Nerve growth factor promotes reorganization of the axonal microtubule array at sites of axon collateral branching." in: Developmental neurobiology, Vol. 75, Issue 12, pp. 1441-61, 2016 (Sample species: Chicken). Further details: Immunocytochemistry,Immunofluorescence

Ma, Zuo, Yang, Ubeda, Gant, Alaverdyan, Kiosea, Nazari, Chen, Nothias, Chan, Teng, Frautschy, Cole: "Loss of MAP function leads to hippocampal synapse loss and deficits in the Morris Water Maze with aging." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 21, pp. 7124-36, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting,Immunohistochemistry (Frozen Sections)

Capoccia, Jin, Kong, Peek, Fassan, Rugge, Mills: "The ubiquitin ligase Mindbomb 1 coordinates gastrointestinal secretory cell maturation." in: The Journal of clinical investigation, Vol. 123, Issue 4, pp. 1475-91, 2013 Further details: Immunocytochemistry,Immunofluorescence

Marchbank, Waters, Roberts, Solomon, Whitehouse: "MAP1B Interaction with the FW Domain of the Autophagic Receptor Nbr1 Facilitates Its Association to the Microtubule Network." in: International journal of cell biology, Vol. 2012, pp. 208014, 2012 (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunofluorescence

Tymanskyj, Lin, Gordon-Weeks: "Evolution of the spatial distribution of MAP1B phosphorylation sites in vertebrate neurons." in: Journal of anatomy, Vol. 216, Issue 6, pp. 692-704, 2010 Further details: Immunocytochemistry,Western Blotting,Immunofluorescence,Immunohistochemistry (Frozen Sections)

Scales, Lin, Kraus, Goold, Gordon-Weeks: "Nonprimed and DYRK1A-primed GSK3 beta-phosphorylation sites on MAP1B regulate microtubule dynamics in growing axons." in: Journal of cell science, Vol. 122, Issue Pt 14, pp. 2424-35, 2009 (Sample species: Rat (Rattus)). Further details: Western Blotting,Immunohistochemistry (Frozen Sections)

Trivedi, Marsh, Goold, Wood-Kaczmar, Gordon-Weeks: "Glycogen synthase kinase-3beta phosphorylation of MAP1B at Ser1260 and Thr1265 is spatially restricted to growing axons." in: Journal of cell science, Vol. 118, Issue Pt 5, pp. 993-1005, 2005 (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence,Immunohistochemistry (Frozen Sections)

Images

Product Details anti-MAP1B Antibody Target Details MAP1B Application Details Handling ProductDetails: References for anti-MAP1B antibody (ABIN449248) back to top
Supplier Images
Western Blotting (WB) image for anti-Microtubule-Associated Protein 1B (MAP1B) (C-Term), (pThr1265) antibody (ABIN449248) Western Blot: MAP1B [p Thr1265] Antibody (SuperBUGS) [NBP1-42827] - Analysis of phosp...
Immunohistochemistry (IHC) image for anti-Microtubule-Associated Protein 1B (MAP1B) (C-Term), (pThr1265) antibody (ABIN449248) Immunohistochemistry: MAP1B [p Thr1265] Antibody (SuperBUGS) [NBP1-42827] - Immunohis...