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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||105 Antibodies||63 Antibodies||60 Antibodies|
|Immunoprecipitation (IP)||10 Antibodies||3 Antibodies||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||22 Antibodies||14 Antibodies||13 Antibodies|
|Immunohistochemistry (IHC)||17 Antibodies||8 Antibodies||7 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||1 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||1 Antibodies||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||32 Antibodies||32 Antibodies|
|Antigen||rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This ROCK1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Product Details anti-ROCK1 AntibodyTarget Details ROCK1 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of Rock-1 protein. (region surrounding asn311)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||Affinity chromatography using epitope-specific immunogen|
|Purity||> 95 % (by SDS-PAGE)|
Target Details ROCK1Product Details anti-ROCK1 Antibody Application Details Handling Images back to top
|Alternative Name||ROCK1 (ROCK1 Antibody Abstract)|
|Background||Rho, the Ras-related small GTPase, is responsible for the regulation of Actinbased cytoskeletal structures including stress fibers, focal adhesions and the contractile RING apparatus. Rho proteins function as molecular switches that are able to turn cytokinesis on and off. Although little is known about signaling downstream of Rho, a host of putative Rho effector proteins have been described, including rhophilin, Rhotekin, citron and the serine/threonine kinase, protein kinase N. Two additional Rho-activated serine/threonine kinases have been described, designated Rock-1 and Rock-2 (also referred to as Roka) for Rho-associated coil-containing protein kinase. Rock-1 and Rock-2 share a structural similarity with myotonic dystrophy kinase.Synonyms: NY-REN-35, ROCK-1, Renal carcinoma antigen NY-REN-35, Rho-associated, Rho-associated protein kinase 1, coiled-coil-containing protein kinase 1, p160ROCK|
|Molecular Weight||approx. 158 kDa|
|Research Area||Cancer, Cell Cycle, Cytoskeleton, Microfilaments, Apoptosis/Necrosis, Signaling|
|Pathways||Microtubule Dynamics, WNT Signaling|
Application DetailsProduct Details anti-ROCK1 Antibody Target Details ROCK1 Handling Images back to top
Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-ROCK1 Antibody Target Details ROCK1 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-ROCK1 Antibody Target Details ROCK1 Application Details Handling back to top