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anti-Zebrafish (Danio rerio) VPS4A antibody for Western Blotting

Recommended VPS4A Antibody (supplied by: Log in to see )

Antigen
Vacuolar Protein Sorting 4 Homolog A (S. Cerevisiae) (VPS4A) Antibodies
  • 4930589C15Rik
  • AI325971
  • AW553189
  • SKD1
  • SKD1A
  • SKD2
  • VPS4
  • VPS4-1
  • vsp4
  • zgc:153907
Epitope
N-Term
4
2
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
27
20
19
1
1
1
Host
Rabbit
25
2
Clonality
Polyclonal
Conjugate
This VPS4A antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
16
11
3
3
2
2
1
Supplier
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Catalog No. ABIN2785950
$ 289.00
Plus shipping costs $45.00

Top referenced anti-VPS4A antibody for Western Blotting

Similar anti-VPS4A Antibodies

Application / Reactivity Human Cow (Bovine) Dog (Canine) Mouse (Murine) Rat (Rattus) Xenopus laevis Zebrafish (Danio rerio)
Western Blotting (WB) 17 Antibodies 2 Antibodies 2 Antibodies 11 Antibodies 10 Antibodies 2 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 3 Antibodies 3 Antibodies 3 Antibodies
Immunohistochemistry (IHC) 3 Antibodies 2 Antibodies 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies 11 Antibodies
Immunofluorescence (IF) 2 Antibodies 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 1 Antibodies

General

Antigen Vacuolar Protein Sorting 4 Homolog A (S. Cerevisiae) (VPS4A) Antibodies
Epitope N-Term
(4), (2), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(27), (20), (19), (1), (1), (1)
Host Rabbit
(25), (2)
Clonality
Polyclonal
Conjugate This VPS4A antibody is un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(16), (11), (3), (3), (2), (2), (1)
Pubmed 1 reference available
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Product Details anti-VPS4A Antibody

Target Details VPS4A Application Details Handling References for anti-VPS4A Antibody (ABIN2785950) Images
Blocking Peptide Blocking peptide for this product available: ABIN986092
Sequence YFLHAIKYEA HSDKAKESIR AKCVQYLDRA EKLKDYLRSK EKHGKKPVKE
Predicted Reactivity African clawed frog : 100 %, Bovine : 100 %, Dog : 100 %, Human : 100 %, Mouse : 100 %, Rabbit : 100 %, Rat : 100 %, Zebrafish : 100 %
Characteristics This is a rabbit polyclonal antibody against VPS4A. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human VPS4A

Target Details VPS4A

Product Details anti-VPS4A Antibody Application Details Handling References for anti-VPS4A Antibody (ABIN2785950) Images back to top
Antigen
Alternative Name VPS4A (VPS4A Antibody Abstract)
Background VPS4A is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast.The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. The mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be really an yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 16, the gene for the other resides on chromosome 18.
Molecular Weight 49 kDa
Gene ID 27183
NCBI Accession NM_013245, NP_037377
UniProt Q9UN37
Pathways Microtubule Dynamics

Application Details

Product Details anti-VPS4A Antibody Target Details VPS4A Handling References for anti-VPS4A Antibody (ABIN2785950) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 437 AA

Restrictions For Research Use only

Handling

Product Details anti-VPS4A Antibody Target Details VPS4A Application Details References for anti-VPS4A Antibody (ABIN2785950) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-VPS4A Antibody (ABIN2785950)

Product Details anti-VPS4A Antibody Target Details VPS4A Application Details Handling Images back to top
Product cited in:

Taylor, Hanson, Kielian: "Ubiquitin depletion and dominant-negative VPS4 inhibit rhabdovirus budding without affecting alphavirus budding." in: Journal of virology, Vol. 81, Issue 24, pp. 13631-9, 2007

Images

Product Details anti-VPS4A Antibody Target Details VPS4A Application Details Handling References for anti-VPS4A Antibody (ABIN2785950) back to top
Supplier Images
Western Blotting (WB) image for anti-VPS4A antibody (Vacuolar Protein Sorting 4 Homolog A (S. Cerevisiae)) (N-Term) (ABIN2785950) WB Suggested Anti-VPS4A Antibody Titration: 0.2-1 ug/ml
ELISA Titer: