We are preparing the requested document. Please wait, this may take a while...!
|Antigen||Interleukin 1, beta (IL1B) ELISA Kits|
Kits with alternative reactivity to:
|Method Type||Sandwich ELISA|
|Detection Range||15.6 pg/mL - 1000 pg/mL|
|Minimum Detection Limit||15.6 pg/mL|
|16 references available|
|Supplier||Log in to see|
Product Details IL1B ELISA KitTarget details Application Details Handling References for IL1B Kit (ABIN415066) Images
|Purpose||The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL1b in Serum,Plasma,Tissue Homogenate,Cell Lysate,Cell Culture Supernatant,Biological Fluids|
|Sample Type||Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids|
This assay has high sensitivity and excellent specificity for detection of Interleukin 1 Beta (IL1b).
|Cross-Reactivity (Details)||No significant cross-reactivity or interference between Interleukin 1 Beta (IL1b) and analogues was observed.|
|Material not included||
Target detailsProduct Details IL1B ELISA Kit Application Details Handling References for IL1B Kit (ABIN415066) Images back to top
|Alternative Name||IL1b (IL1B ELISA Kit Abstract)|
|Pathways||NF-kappaB Signaling, Interferon-gamma Pathway, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy|
Application DetailsProduct Details IL1B ELISA Kit Target details Handling References for IL1B Kit (ABIN415066) Images back to top
Information on standard material:
|Sample Volume||100 μL|
|Assay Time||3 h|
|Protocol||The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Beta (IL1b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Beta (IL1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Beta (IL1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Beta (IL1b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.|
Serum: Allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 20 minutes at approximately 1000 × g. Assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Plasma: Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000 × g within 30 minutes of collection. Remove plasma and assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Tissue Homogenates: The preparation of tissue homogenates will vary depending upon tissue type. For this assay, rinse tissues in ice-cold PBS (0.02mol/L,pH 7.0-7.2) to remove excess blood thoroughly and weigh before homogenization. Mince the tissues to small pieces and homogenize them in 5-10 mL of PBS with a glass homogenizer on ice (Micro Tissue Grinders work, too). Sonicate the resulting suspension with an ultrasonic cell disrupter or subject it to two freeze-thaw cycles to further break the cell membranes. Centrifugate the homogenates for 5 minutes at 5000 × g. Remove the supernate and assay immediately or aliquot and store at -20°C
Cell Lysate: Cells must be lysed before assaying according to the following directions. Adherent cells should be detached with trypsin and then collected by centrifugation (suspension cells can be collected by centrifugation directly). Wash cells three times in cold PBS. Resuspend cells in PBS (1×) and subject them to ultrasonication for 4 times (or Freeze cells at -20 °C. Thaw cells with gentle mixing. Repeat the freeze/thaw cycle for 3 times.) Centrifuge at 1500 × g for 10 minutes at 2 - 8°C to remove cellular debris.
Cell Culture Supernatant: Centrifuge samples for 20 minutes at 1000 × g. Remove particulates and assay immediately or store samples in aliquot at -20 °C or -80 °C for later use. Avoid repeated freeze/thaw cycles.
Biological Fluids: Centrifuge samples for 20 minutes at 1000 × g. Remove particulates and assay immediately or store samples in aliquot at -20 °C or -80 °C for later use. Avoid repeated freeze/thaw cycles.
|Calculation of Results||
Average the duplicate readings for each standard, control, and samples and subtract the average zero standard optical density. Construct a standard curve by plotting the mean O.D. and concentration for each standard and draw a best fit curve through the points on the graph or create a standard curve on log-log graph paper with IL1b concentration on the y-axis and absorbance on the x-axis. Using some plot software, for instance, curve expert 1.30, is also recommended. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
In order to make the calculation easier, we plot the O.D. value of the standard (X-axis) against the known concentration of the standard (Y-axis), although concentration is the independent variable and O.D. value is the dependent variable. However, the O.D. values of the standard curve may vary according to the conditions of assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), plotting log of the data to establish standard curve for each test is recommended. Typical standard curve below is provided for reference only.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Beta (IL1b) were tested 20 times on one plate, respectively.
|Restrictions||For Research Use only|
HandlingProduct Details IL1B ELISA Kit Target details Application Details References for IL1B Kit (ABIN415066) Images back to top
|Precaution of Use||The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.|
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
|Expiry Date||6 months|
References for IL1B Kit (ABIN415066)Product Details IL1B ELISA Kit Target details Application Details Handling Images back to top
|Product cited in:||
Mern, Tschugg, Hartmann, Thomé et al.: "Self-complementary adeno-associated virus serotype 6 mediated knockdown of ADAMTS4 induces long-term and effective enhancement of aggrecan in degenerative human nucleus pulposus cells: A new ..." in: PLoS ONE, Vol. 12, Issue 2, pp. e0172181, 2017
Abduljabbar, Vohra, Kellesarian, Javed et al.: "Efficacy of scaling and root planning with and without adjunct Nd:YAG laser therapy on clinical periodontal parameters and gingival crevicular fluid interleukin 1-beta and tumor necrosis factor-alpha ..." in: Journal of photochemistry and photobiology. B, Biology, Vol. 169, pp. 70-74, 2017
Zhong, Liu, Zhang, Zhang, Liu, Hu: "Alterations in gut microbiota during remission and recurrence of diabetes after duodenal-jejunal bypass in rats." in: World journal of gastroenterology, Vol. 22, Issue 29, pp. 6706-15, 2016
Tian, Peng, Zhong, Yang, Pang, Lou, Li, An, Zhang, Xu, Dong: "β-Caryophyllene protects in vitro neurovascular unit against oxygen-glucose deprivation and re-oxygenation-induced injury." in: Journal of neurochemistry, Vol. 139, Issue 5, pp. 757-768, 2016
Kumar, Kumar, Gupta: "Manganese- and cobalt-based coordination networks as promising heterogeneous catalysts for olefin epoxidation reactions." in: Inorganic chemistry, Vol. 54, Issue 6, pp. 2603-15, 2015
Louzada, Araújo, Verma, Daolio, Herrmann, Tomassini: "Critical cooperation range to improve spatial network robustness." in: PLoS ONE, Vol. 10, Issue 3, pp. e0118635, 2015
Meuleman, Snaterse, van Beelen, Anholts, Pilgram, van der Westerlaken, Eikmans, Claas: "The immunomodulating effect of seminal plasma on T cells." in: Journal of reproductive immunology, Vol. 110, pp. 109-16, 2015
Zhu, Zhao, Chen, Xu, Hu, Guo, Li, Zhang, Chang, Song, Xiong, Chang, Li: "ACAT1 regulates the dynamics of free cholesterols in plasma membrane which leads to the APP-α-processing alteration." in: Acta biochimica et biophysica Sinica, Vol. 47, Issue 12, pp. 951-9, 2015
Liu, Qu, Li, Tang, Yang, Wang, Deng: "Relationship between serum secreted frizzled-related protein 4 levels and the first-phase of glucose-stimulated insulin secretion in individuals with different glucose tolerance." in: Endocrine journal, Vol. 62, Issue 8, pp. 733-40, 2015
Yang, Yang, Liang, Zhu: "MicroRNA-155 Promotes Atherosclerosis Inflammation via Targeting SOCS1." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 36, Issue 4, pp. 1371-81, 2015
Sun, Liu, Huan, Zhang: "Intracranial injection of recombinant stromal-derived factor-1 alpha (SDF-1?) attenuates traumatic brain injury in rats." in: Inflammation research : official journal of the European Histamine Research Society ... [et al.], Vol. 63, Issue 4, pp. 287-97, 2014
Schwarz, Mihatovic, Golubovic, Eick, Iglhaut, Becker: "Experimental peri-implant mucositis at different implant surfaces." in: Journal of clinical periodontology, Vol. 41, Issue 5, pp. 513-20, 2014
Pourabbas, Kashefimehr, Rahmanpour, Babaloo, Kishen, Tenenbaum, Azarpazhooh: "Effects of photodynamic therapy on clinical and gingival crevicular fluid inflammatory biomarkers in chronic periodontitis: a split-mouth randomized clinical trial." in: Journal of periodontology, Vol. 85, Issue 9, pp. 1222-9, 2014
Yang, Cheng, Tian, Zhou, Wu, Xu, Fang, Fang, Xu: "MRTF-A steers an epigenetic complex to activate endothelin-induced pro-inflammatory transcription in vascular smooth muscle cells." in: Nucleic acids research, Vol. 42, Issue 16, pp. 10460-72, 2014
Wu, Peng, Du, Lin, Liu et al.: "GYY4137, a hydrogen sulfide?releasing molecule, inhibits the inflammatory response by suppressing the activation of nuclear factor?kappa B and mitogen?activated protein kinases in Coxsackie virus ..." in: Molecular medicine reports, 2014
Mern, Fontana, Beierfuß, Thomé, Hegewald et al.: "A combinatorial relative mass value evaluation of endogenous bioactive proteins in three-dimensional cultured nucleus pulposus cells of herniated intervertebral discs: identification of potential ..." in: PLoS ONE, Vol. 8, Issue 11, pp. e81467, 2013
ImagesProduct Details IL1B ELISA Kit Target details Application Details Handling References for IL1B Kit (ABIN415066) back to top