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Human IL1B ELISA Kit

Recommended IL1B Product (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) ELISA Kits
  • IL-1
  • IL-1B
  • Il-1b
  • IL-1beta
  • IL-1BETA
  • il1-b
  • IL1-BETA
  • IL1B
  • IL1beta
  • IL1F2
  • zgc:111873
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
72
44
36
13
10
9
8
8
7
7
6
4
3
2
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
8.2-2000 pg/mL
Minimum Detection Limit
8.2 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN2506948
$ 433.40
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.30628014 ABIN415066 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see 12
0.30628014 ABIN411293 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate) 3.9-250 pg/mL 3.9 pg/mL Log in to see 7
0.30628014 ABIN924850 Sandwich ELISA Cell Lysate, Serum, Plasma 4-250 pg/mL 4 pg/mL Log in to see
0.30628014 ABIN2472076 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant 7.81-500 pg/mL 7.81 pg/mL Log in to see
0.30628014 ABIN1115635 Sandwich ELISA Serum, Plasma, Biological Fluids 7.813-500 pg/mL 7.81 pg/mL Log in to see
0.30628014 ABIN1888524 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine 0.3-100 pg/mL 0.3 pg/mL Log in to see
0.30628014 ABIN2708254 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 3.9-250 pg/mL 3.9 pg/mL Log in to see
0.30628014 ABIN2708255 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 7.8-500 pg/mL 7.8 pg/mL Log in to see
0.30628014 ABIN2762270 Cell Culture Supernatant, Serum, Plasma (EDTA), Plasma (citrate), Plasma (heparin) 7.8-500 pg/mL 7.8 pg/mL Log in to see
0.30628014 ABIN2114188 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin - citrate) Log in to see
0.0012155735 ABIN365160 Sandwich ELISA Serum, Cell Culture Supernatant, Urine, Cerebrospinal Fluid 125-8000 pg/mL 125 pg/mL Log in to see 4
0.0012155735 ABIN509710 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 50-1000 pg/mL 50 pg/mL Log in to see 1
0.0012155735 ABIN3032937 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 0.41-100 pg/mL 0.41 pg/mL Log in to see
0.0012155735 ABIN625019 Serum, Plasma, Cell Culture Log in to see 25
0.0012155735 ABIN1028979 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 3.125-200 pg/mL 3.125 pg/mL Log in to see
0.0012155735 ABIN512545 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 50-1000 pg/mL 50 pg/mL Log in to see
0.0012155735 ABIN1446046 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Biological Samples Log in to see 5
0.0012155735 ABIN455553 Cell Culture Supernatant, Serum, Plasma, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012155735 ABIN2690563 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant Log in to see 2
0.0012155735 ABIN2684632 Sandwich ELISA Plasma (citrate), Plasma (EDTA), Plasma (heparin), Cell Culture Supernatant, Serum 3.9-250 pg/mL 3.9 pg/mL Log in to see 4

Top referenced IL1B ELISA Kit for ELISA

  • Human IL1B ELISA Kit - ABIN625019 (25 Publications): Dinarello: Interleukin-1 and interleukin-1 antagonism. in Blood 1991 (PubMed)

  • Human IL1B ELISA Kit for Sandwich ELISA - ABIN415066 (12 Publications): Sun, Liu, Huan, Zhang: Intracranial injection of recombinant stromal-derived factor-1 alpha (SDF-1?) attenuates traumatic brain injury in rats. in Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2014 (PubMed)

  • Human IL1B ELISA Kit for Sandwich ELISA - ABIN1446046 (5 Publications): Markiewicz, Pytel, Mucha, Szymanek, Szaflik, Szaflik, Majsterek: Altered Expression Levels of MMP1, MMP9, MMP12, TIMP1, and IL-1β as a Risk Factor for the Elevated IOP and Optic Nerve Head Damage in the Primary Open-Angle Glaucoma Patients. in BioMed research international 2015 (PubMed)

  • Human IL1B ELISA Kit for Sandwich ELISA - ABIN365160 (4 Publications): Wu, Xu, Lu, Ma, Miao, Liu, Cheng, Feng, Liu, Feng-Ding, Na-Liu, Li, Zhao: Correlation of AIM2 expression in peripheral blood mononuclear cells from humans with acute and chronic hepatitis B. in Human immunology 2013 (PubMed)

  • Human IL1B ELISA Kit for Sandwich ELISA - ABIN2684632 (4 Publications): Baek, Ohgi, Rose, Koo, Glass, Rosenfeld: Exchange of N-CoR corepressor and Tip60 coactivator complexes links gene expression by NF-kappaB and beta-amyloid precursor protein. in Cell 2002 (PubMed)

  • Human IL1B ELISA Kit for Sandwich ELISA - ABIN2690563 (2 Publications): Kitamura, Takaku, Miyajima: IL-1 up-regulates the expression of cytokine receptors on a factor-dependent human hemopoietic cell line, TF-1. in International immunology 1991 (PubMed)

  • Human IL1B ELISA Kit for Sandwich ELISA - ABIN509710 (1 Publications): Teng, Zhang, Liu, Zhou, Wang, Zhuang, Huang, Hong: miR-143 inhibits interleukin-13-induced inflammatory cytokine and mucus production in nasal epithelial cells from allergic rhinitis patients by targeting IL13R?1. in Biochemical and biophysical research communications 2015 (PubMed)

  • Human IL1B ELISA Kit - ABIN1446199 (1 Publications): Lu, Nossent: Thrombopoietin levels in systemic lupus erythematosus are linked to inflammatory cytokines, but unrelated to thrombocytopenia or thrombosis. in Lupus 2014 (PubMed)

Similar IL1B ELISA Kits

Application / Reactivity Human
ELISA 53 ELISA Kits

General

Antigen Interleukin 1, beta (IL1B) ELISA Kits
Reactivity Human
Kits with alternative reactivity to:
(72), (44), (36), (13), (10), (9), (8), (8), (7), (7), (6), (4), (3), (2), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 8.2-2000 pg/mL
Minimum Detection Limit 8.2 pg/mL
Application ELISA
Supplier Log in to see

Product Details IL1B ELISA Kit

Target details Application Details Handling Images
Purpose The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of IL-1 beta in human serum, plasma, and other biological fluids not being tested
Brand EasyTest™
Sample Type Serum, Plasma
Detection Method Colorimetric
Specificity This assay has high sensitivity and excellent specificity for detection of human IL-1 beta
Sensitivity <1 pg/mL
Characteristics one wash
Components
  • Assay plate (12x8 coated Microwells)-1
  • Standard-2x 10μL
  • human IL-1 beta Mix-4x 8 μL
  • Assay diluent (5x concentrate)-1 x15mL
  • Wash buffer (20x concentrate)-1x25 mL
  • TMB substrate-1x12mL
  • Stop solution-1x8mL
  • Adhensive strip-2
  • Instruction manual-1.
Material not included 1. Distilled or deionized water, 2.Precision pipettes, with disposable plastic tips, 3.Beakers, flasks, cylinders necessary for preparation of reagents, 4.Microplate washing device (multichannel pipette or automated microplate washer), 5.Microplate shaker, 6.Microplate reader capable of reading at 450 nm.

Target details

Product Details IL1B ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name IL-1 beta (IL1B ELISA Kit Abstract)
Background IL-1 is a name that designates two pleiotropic cytokines, IL-1α and IL-1β, which are the products of distinct genes. IL-1α and IL-1β are structurally related polypeptides that share approximately 21 % amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins.
Molecular Weight 17 kDa
Gene ID 3553
NCBI Accession NP_000567
UniProt P01584
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway

Application Details

Product Details IL1B ELISA Kit Target details Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Sample Volume 50 μL
Assay Time 1.5 h
Plate Strips (12 x 8)
Protocol This assay employs the fast quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-1 beta has been pre-coated onto a microplate. Standards, samples and human IL-1 beta mix are pipetted into the wells and any IL-1 beta present is bound by the immobilized antibody. Following a wash to remove any unbound reagent, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 beta bound in the initial step. The color development is stopped and the intensity of the color is measured.
Reagent Preparation 1. Assay diluent: Dilute the concentrated assay diluent 1:5 with distilled water (e.g. 10 mL plus 40 mL). 2. Wash buffer: Dilute the concentrated wash buffer 1:20 with distilled water (e.g. 20 mL plus 380 mL). 3. Standard: Briefly spin standard vial before use. Add 490 μL 1x Assay Diluent to prepare a 5 ng/mL standard. Gently vortex to mix.Take 200 μL IL-1 beta standard into a tube, then add 300 μL 1x Assay Diluent to prepare a 2000 pg/mL stock standard solution.Add 150 μL 1x Assay Diluent to 7 tubes. Perform serial dilutions by adding 100 μL of each standard to the next tube and vortexing between each transfer, the last tube with 1x assay diluent is the blank as 0pg/mL. 4. human IL-1 beta Mix: Within 15 minutes prior to use, briefly spin the vial. Add 1492 μL of 1x Assay diluent to the vial and mix by pipetting. A vial mix can be used for about 30 wells.
Sample Collection Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4 °C before centrifugation for 15 minutes at 1000 x g. Remove serum and assay immediately or aliquot and store samples at -20 °C or -80 °C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or citrate or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 x g at 2-8 °C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20 °C or -80 °C. Avoid repeated freeze-thaw cycles.
Sample Preparation Levels of the target protein may vary among different specimens. Optimal dilution factors for each sample must be determined by the investigator,The dilution scheme is only suggestion: the recommended dilution for serum and plasma is 1:2.
Assay Procedure 1. All reagents must be brought to room temperature (18-25 °C) prior to use 2. Prepare all reagents, working standards,samples and mix as directed in the respective sections. 3.Determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4 °C. 4. Add 50μL of standard or sample to the designated wells, Gentlely shake/tap the plate for 5 second to mix. 5. Add 50 μL of IL-1 beta Mix into all wells, including the blank wells, cover the plate with adhensive strip. Incubate for 1 hour at room temperature with gentle shaking. 6.Decant or aspirate contents of wells. Wash wells by filling with at least 300 μL/well prepared wash buffer followed by decanting/aspirating. Soak wells in wash buffer for 30 seconds to 1 minute for each wash. Repeat wash 4 times for a total of 5 washes. After the last wash, blot plate on absorbent paper to remove residual buffer. Thorough washing at this step is very important, complete removal of liquid is required for proper performance. 7.Pipette 100 μL of TMB Substrate Solution to each well. Incubate plate for 15 minutes at room temperature in the dark with gentle shaking. 8. Add 50μL of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 9. Determine the optical density of each well within 15 minutes using a microplate reader set to 450nm. If wavelength correction is available, set to 570nm. Subtract readings at 570nm from the readings at 450nm. This subtraction will correct for optical imperfections in the plate.
Calculation of Results 1. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.2. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the target antigen concentration versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. 3. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
Assay Precision intra CV<10%, inter CV <15%
Restrictions For Research Use only

Handling

Product Details IL1B ELISA Kit Target details Application Details Images back to top
Buffer 0.05 % Proclin 300
Preservative ProClin
Precaution of Use The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.
Storage 4 °C/-20 °C

Images

Product Details IL1B ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for IL1B ELISA Kit (Interleukin 1, beta) (ABIN2506948) Interleukin 1, beta (IL1B) ELISA Kit