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|Application / Reactivity||Rat (Rattus)|
|ELISA||33 ELISA Kits|
|Antigen||Interleukin 1, beta (IL1B) ELISA Kits|
|Reactivity||Rat (Rattus) Alternatives|
Kits with alternative reactivity to:
|Methode Type||Sandwich ELISA|
|Detection Range||6.25-400 pg/mL|
|Minimum Detection Limit||6.25 pg/mL|
|20 references available|
|Supplier||Log in to see|
Product Details IL1B ELISA KitTarget details Application Details Handling References for IL1B Kit (ABIN416397) Images
|Purpose||The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL1b in Serum,Plasma,Tissue Homogenate,Cell Lysate,Cell Culture Supernatant,Biological Fluids|
|Sample Type||Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids|
|Specificity||This assay has high sensitivity and excellent specificity for detection of Interleukin 1 Beta (IL1b).|
|Cross-Reactivity (Details)||No significant cross-reactivity or interference between Interleukin 1 Beta (IL1b) and analogues was observed.|
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations - the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
|Material not included||
Target detailsProduct Details IL1B ELISA Kit Application Details Handling References for IL1B Kit (ABIN416397) Images back to top
|Alternative Name||IL1b (IL1B ELISA Kit Abstract)|
|Pathways||NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway|
Application DetailsProduct Details IL1B ELISA Kit Target details Handling References for IL1B Kit (ABIN416397) Images back to top
Information on standard material:
|Sample Volume||100 μL|
|Assay Time||4.5 h|
|Plate||Pre-coated,Strips (12 x 8)|
The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL1b. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for IL1b.
Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.
After TMB substrate solution is added, only those wells that contain IL1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color.
The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 10 nm. The concentration of IL1b in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Serum: Allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 20 minutes at approximately 1000 × g. Assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Plasma: Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000 × g within 30 minutes of collection. Remove plasma and assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Tissue Homogenates: The preparation of tissue homogenates will vary depending upon tissue type. For this assay, rinse tissues in ice-cold PBS (0.02mol/L,pH 7.0-7.2) to remove excess blood thoroughly and weigh before homogenization. Mince the tissues to small pieces and homogenize them in 5-10 mL of PBS with a glass homogenizer on ice (Micro Tissue Grinders work, too). Sonicate the resulting suspension with an ultrasonic cell disrupter or subject it to two freeze-thaw cycles to further break the cell membranes. Centrifugate the homogenates for 5 minutes at 5000 × g. Remove the supernate and assay immediately or aliquot and store at -20°C
Cell Lysate: Cells must be lysed before assaying according to the following directions. Adherent cells should be detached with trypsin and then collected by centrifugation (suspension cells can be collected by centrifugation directly). Wash cells three times in cold PBS. Resuspend cells in PBS (1×) and subject them to ultrasonication for 4 times (or Freeze cells at -20 °C. Thaw cells with gentle mixing. Repeat the freeze/thaw cycle for 3 times.) Centrifuge at 1500 × g for 10 minutes at 2 - 8°C to remove cellular debris.
Cell Culture Supernatant: Centrifuge samples for 20 minutes at 1000 × g. Remove particulates and assay immediately or store samples in aliquot at -20 °C or -80 °C for later use. Avoid repeated freeze/thaw cycles.
Biological Fluids: Centrifuge samples for 20 minutes at 1000 × g. Remove particulates and assay immediately or store samples in aliquot at -20 °C or -80 °C for later use. Avoid repeated freeze/thaw cycles.
|Calculation of Results||
Average the duplicate readings for each standard, control, and samples and subtract the average zero standard optical density. Construct a standard curve by plotting the mean O.D. and concentration for each standard and draw a best fit curve through the points on the graph or create a standard curve on log-log graph paper with IL1b concentration on the y-axis and absorbance on the x-axis. Using some plot software, for instance, curve expert 1.30, is also recommended. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
In order to make the calculation easier, we plot the O.D. value of the standard (X-axis) against the known concentration of the standard (Y-axis), although concentration is the independent variable and O.D. value is the dependent variable. However, the O.D. values of the standard curve may vary according to the conditions of assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), plotting log of the data to establish standard curve for each test is recommended. Typical standard curve below is provided for reference only.
Intra-assay Precision (precision within an assay): 3 samples with low, middle and high level rat IL1b were tested 20 times on one plate, respectively.
Inter-assay Precision (precision between assays): 3 samples with low, middle and high level rat IL1b were tested on 3 different plates, 8 replicates in each plate.
CV (%) = SD/mean X 100
|Restrictions||For Research Use only|
HandlingProduct Details IL1B ELISA Kit Target details Application Details References for IL1B Kit (ABIN416397) Images back to top
|Precaution of Use||The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.|
|Handling Advice||To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.|
|Storage||4 °C/-20 °C|
|Expiry Date||6 months|
References for IL1B Kit (ABIN416397)Product Details IL1B ELISA Kit Target details Application Details Handling Images back to top
|Product cited in:||
Zhao, Zheng, Hu, Shen, Li: "Betulin attenuates kidney injury in septic rats through inhibiting TLR4/NF-κB signaling pathway." in: Life sciences, Vol. 144, pp. 185-93, 2016
Hashimoto, Kim, Oishi, Chen, Iskender, Sakamoto, Ohsumi, Guan, Hwang, Waddell, Cypel, Liu, Keshavjee: "Annexin V homodimer protects against ischemia reperfusion-induced acute lung injury in lung transplantation." in: The Journal of thoracic and cardiovascular surgery, Vol. 151, Issue 3, pp. 861-8, 2016
Zhao, Zhao, Wang, Li, Zhang: "Glycyrrhizic Acid Prevents Sepsis-Induced Acute Lung Injury and Mortality in Rats." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 64, Issue 2, pp. 125-37, 2016
Diandong, Feng, Zaifu, Helland, Weixin, Liping: "Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in rats." in: International journal of immunopathology and pharmacology, Vol. 29, Issue 1, pp. 76-83, 2016
Lin, Wang, Hsu, Liao, Lin, Hsueh: "Molecular Mechanisms Responsible for Neuron-Derived Conditioned Medium (NCM)-Mediated Protection of Ischemic Brain." in: PLoS ONE, Vol. 11, Issue 1, pp. e0146692, 2016
Gokce, Kahveci, Gokce, Sargon, Kisa, Aksoy, Cemil, Erdogan: "Curcumin Attenuates Inflammation, Oxidative Stress, and Ultrastructural Damage Induced by Spinal Cord Ischemia-Reperfusion Injury in Rats." in: Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association, Vol. 25, Issue 5, pp. 1196-207, 2016
Tian, Xiao, Yu, Gu, Zhao, Lü: "The inhibition of transforming growth factor beta-activated kinase 1 contributed to neuroprotection via inflammatory reaction in pilocarpine-induced rats with epilepsy." in: Neuroscience, Vol. 325, pp. 111-23, 2016
Hohl, Linz, Fries, Müller, Stroeder, Urban, Speer, Geisel, Hummel, Laufs, Schirmer, Böhm, Mahfoud: "Modulation of the sympathetic nervous system by renal denervation prevents reduction of aortic distensibility in atherosclerosis prone ApoE-deficient rats." in: Journal of translational medicine, Vol. 14, Issue 1, pp. 167, 2016
Xie, Yan, Lou, Chen: "Effect of ozone on vascular endothelial growth factor (VEGF) and related inflammatory cytokines in rats with diabetic retinopathy." in: Genetics and molecular research : GMR, Vol. 15, Issue 2, 2016
Wang, Wu, Yu, Zhou, Ge, Chi, Cai: "Dexmedetomidine Protects Rat Liver against Ischemia-Reperfusion Injury Partly by the α2A-Adrenoceptor Subtype and the Mechanism Is Associated with the TLR4/NF-κB Pathway." in: International journal of molecular sciences, Vol. 17, Issue 7, 2016
Liu, Wang, Wei, Fan, Lu, Zhu, Chen, Huang, Zhou, Zheng: "Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery." in: International immunopharmacology, Vol. 32, pp. 66-75, 2016
Cong, Chen: "Neuroprotective Effect of Ginsenoside Rd in Spinal Cord Injury Rats." in: Basic & clinical pharmacology & toxicology, Vol. 119, Issue 2, pp. 193-201, 2016
Salamanna, Frizziero, Pagani, Giavaresi, Curzi, Falcieri, Marini, Abruzzo, Martini, Fini: "Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes." in: Connective tissue research, Vol. 56, Issue 1, pp. 35-43, 2015
Hu, Gu, Sun, Zhang, Gao, Yang, Yuan: "Ursolic acid improves survival and attenuates lung injury in septic rats induced by cecal ligation and puncture." in: The Journal of surgical research, Vol. 194, Issue 2, pp. 528-36, 2015
Yang, Ni, Wang, Du, Zhang, Zhang, Zhang, Jiang: "Melatonin attenuates intestinal ischemia--reperfusion-induced lung injury in rats by upregulating N-myc downstream-regulated gene 2." in: The Journal of surgical research, Vol. 194, Issue 1, pp. 273-80, 2015
He, Tang, Gao, Wu, Feng, Lin, Wu: "Tanshinone IIA attenuates bleomycin-induced pulmonary fibrosis in rats." in: Molecular medicine reports, Vol. 11, Issue 6, pp. 4190-6, 2015
Lin, Kong, Wu, Yang, Ji: "Effect of TLR4/MyD88 signaling pathway on expression of IL-1? and TNF-? in synovial fibroblasts from temporomandibular joint exposed to lipopolysaccharide." in: Mediators of inflammation, Vol. 2015, pp. 329405, 2015
Liu, Zhang, Zhao, Chang, Li, Yu, Zhang, Zhang: "Melanocortin MC4 receptor agonists alleviate brain damage in abdominal compartment syndrome in the rat." in: Neuropeptides, Vol. 49, pp. 55-61, 2015
You, Zhang, Gong, Chen, Li, Yang, Liu: "Mesenchymal stromal cell-dependent reprogramming of Kupffer cells is mediated by TNF-α and PGE2 and is crucial for liver transplant tolerance." in: Immunologic research, Vol. 62, Issue 3, pp. 292-305, 2015
Chen, Wei, Wang, Zhu, Li, Olunga, Gao, Fan: "Cardioprotection against ischemia/reperfusion injury by QiShenYiQi Pill® via ameliorate of multiple mitochondrial dysfunctions." in: Drug design, development and therapy, Vol. 9, pp. 3051-66, 2015
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