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|Antigen||Interleukin 1, beta (IL1B) ELISA Kits|
|Reactivity||Rat (Rattus) Alternatives|
Kits with alternative reactivity to:
|Method Type||Sandwich ELISA|
|Detection Range||80-50000 pg/mL|
|Minimum Detection Limit||80 pg/mL|
|20 references available|
|Supplier||Log in to see|
Product Details IL1B ELISA KitTarget details Application Details Handling References for IL1B Kit (ABIN625200) Images
|Purpose||Rat IL-1 beta (IL-1 F2) ELISA Kit for cell culture supernatants, plasma, and serum samples.|
|Sample Type||Plasma, Cell Culture Supernatant, Serum|
|Specificity||This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta- NGF, TIMP-1, TNF-alpha.|
|Cross-Reactivity (Details)||This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1alpha, IL-1beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3alpha, beta- NGF, TIMP-1, TNF-alpha.|
|Sensitivity||< 80 pg/mL|
|Material not included||
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|Alternative Name||IL-1 beta (IL1B ELISA Kit Abstract)|
|Background||Interleukin-1 beta (IL-1 beta)|
|Research Area||Cytokines, Immunology, Virology, Inflammation, Cancer|
|Pathways||NF-kappaB Signaling, Interferon-gamma Pathway, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy|
Application DetailsProduct Details IL1B ELISA Kit Target details Handling References for IL1B Kit (ABIN625200) Images back to top
|Application Notes||Recommended Dilution for serum and plasma samples2 fold|
|Sample Volume||100 μL|
1. Bring all reagents and samples to room temperature (18 - 25 °C) before use.
2. Sample dilution: Assay Diluent A (Item D) should be used for dilution of serum/plasma samples. 1x Assay Diluent B (Item E) should be used for dilution of cell culture supernates. Suggested dilution for normal serum/plasma: 2 fold*. * Please note that levels of the target protein may vary between different specimens. Optimal dilution factors for each sample must be determined by the investigator.
3. Assay Diluent B should be diluted 5-fold with deionized or distilled water before use.
4. Preparation of standard: Briefly spin the vial of Item C and then add 400 µL Assay Diluent A (for serum/plasma samples) or 1x Assay Diluent B (for cell culture supernates) into Item C vial to prepare a 50,000 pg/mL standard. Dissolve the powder thoroughly by a gentle mix. Pipette 260 µL Assay Diluent A or 1x Assay Diluent B into each tube. Use the 50,000 pg/mL standard solution to produce a dilution series . Mix each tube thoroughly before the next transfer. Assay Diluent A or 1x Assay Diluent B serves as the zero standard (0 pg/mL). Standard vial + 400 µL 130 µL 130 µL 130 µL 130 µL 130 µL 130myl 50,000 16,667 5556 1852 617.3 205.8 68.59 0 pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL
5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to yield 400 ml of 1x Wash Buffer.
6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µL of 1x Assay Diluent B into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4 °C for 5 days). The detection antibody concentrate should be diluted 80-fold with 1x Assay Diluent B and used in step 4 of Part VI Assay Procedure.
7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) and pipette up and down to mix gently before use. HRP-Streptavidin concentrate should be diluted 200-fold with 1x Assay Diluent B. For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 50 µL of HRP-Streptavidin concentrate into a tube with 10 ml 1x Assay Diluent B to prepare a 200-fold diluted HRP- Streptavidin solution (don't store the diluted solution for next day use). Mix well.
1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. It is recommended that all standards and samples be run at least in duplicate.
2. Add 100 µL of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4 °C with gentle shaking.
3. Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each well with Wash Buffer (300 myl) using a multi-channel pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
4. Add 100 µL of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking.
5. Discard the solution. Repeat the wash as in step
6. Add 100 µL of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking.
7. Discard the solution. Repeat the wash as in step
8. Add 100 µL of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking.
9. Add 50 µL of Stop Solution (Item I) to each well. Read at 450 nm immediately.
|Calculation of Results||
Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
Typical Data: These standard curves are for demonstration only. A standard curve must be run with each assay. Assay Diluent A Rat IL-1beta concentration (pg/mL) O D =4 50 (n m ) 0.01 0.1 1 10 10 100 1,000 10,000 100,000 Assay Diluent B Rat IL-1beta concentration (pg/mL) O D =4 50 (n m ) 0.001 0.01 0.1 1 10 10 100 1,000 10,000 100,000
Sensitivity: The minimum detectable dose of IL-1 beta is typically less than 80 pg/mL.
Recovery: Recovery was determined by spiking various levels of rat IL-1 beta into serum, plasma and cell culture media. Mean recoveries are as follows: Sample Type Average % Recovery Range ( %) Serum 107.2 95-115 Plasma 97.55 89-108 Cell culture media 92.89 81-109
Linearity: Sample Type Serum Plasma Cell Culture Media 1:2 Average % of Expected 107.3 103.5 132.2 Range ( %) 93-114 91-113 120-139 1:4 Average % of Expected 75.11 75.63 127.9 Range ( %) 68-88 68-89 114-140
Reproducibility: Intra-Assay: CV<10 % Inter-Assay: CV<12 %
|Assay Precision||Intra-Assay: CV< 10 % Inter-Assay: CV< 12 %|
|Restrictions||For Research Use only|
HandlingProduct Details IL1B ELISA Kit Target details Application Details References for IL1B Kit (ABIN625200) Images back to top
|Handling Advice||Avoid repeated freeze-thaw cycles.|
|Storage Comment||The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.|
|Expiry Date||6 months|
References for IL1B Kit (ABIN625200)Product Details IL1B ELISA Kit Target details Application Details Handling Images back to top
|Product cited in:||
Sil, Ghosh, Sanyal, Guha, Ghosh: "A comparison of neurodegeneration linked with neuroinflammation in different brain areas of rats after intracerebroventricular colchicine injection." in: Journal of immunotoxicology, Vol. 13, Issue 2, pp. 181-90, 2016
Begum, Sharma, Pillai, Aeri, Sheliya: "Inhibitory effect of Careya arborea on inflammatory biomarkers in carrageenan-induced inflammation." in: Pharmaceutical biology, Vol. 53, Issue 3, pp. 437-45, 2015
Abdelkader, Safar, Salem: "Ursodeoxycholic Acid Ameliorates Apoptotic Cascade in the Rotenone Model of Parkinson's Disease: Modulation of Mitochondrial Perturbations." in: Molecular neurobiology, 2015
Sharma, Deshmukh: "Vinpocetine attenuates MPTP-induced motor deficit and biochemical abnormalities in Wistar rats." in: Neuroscience, Vol. 286, pp. 393-403, 2015
Khan, Jamwal, Bijjem, Prakash, Kumar: "Neuroprotective effect of hemeoxygenase-1/glycogen synthase kinase-3? modulators in 3-nitropropionic acid-induced neurotoxicity in rats." in: Neuroscience, Vol. 287C, pp. 66-77, 2015
Tyagi, Bisht, Pant, Kumar, Majeed, Prakash: "Possible role of GABA-B receptor modulation in MPTP induced Parkinson's disease in rats." in: Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie, Vol. 67, Issue 2, pp. 211-7, 2015
Yaidikar, Thakur: "Punicalagin attenuated cerebral ischemia-reperfusion insult via inhibition of proinflammatory cytokines, up-regulation of Bcl-2, down-regulation of Bax, and caspase-3." in: Molecular and cellular biochemistry, Vol. 402, Issue 1-2, pp. 141-8, 2015
Kumar, Sharma, Prashar, Deshmukh: "Effect of licofelone--a dual COX/5-LOX inhibitor in intracerebroventricular streptozotocin-induced behavioral and biochemical abnormalities in rats." in: Journal of molecular neuroscience : MN, Vol. 55, Issue 3, pp. 749-59, 2015
Sayd, Antón, Alén, Caso, Pavón, Leza, Rodríguez de Fonseca, García-Bueno, Orio: "Systemic administration of oleoylethanolamide protects from neuroinflammation and anhedonia induced by LPS in rats." in: The international journal of neuropsychopharmacology / official scientific journal of the Collegium Internationale Neuropsychopharmacologicum (CINP), Vol. 18, Issue 6, 2015
Chen, Min, Wang, Leung, Shi, Zhou, Yu, Wang, An, Sha, Chen: "Pre-activation of mesenchymal stem cells with TNF-?, IL-1? and nitric oxide enhances its paracrine effects on radiation-induced intestinal injury." in: Scientific reports, Vol. 5, pp. 8718, 2015
Ramadan, El-Beih, Talaat, Abd El-Ghffar: "Anti-inflammatory activity of green versus black tea aqueous extract in a rat model of human rheumatoid arthritis." in: International journal of rheumatic diseases, 2015
Salama, Al-Harbi, Abdel-Bakky, Omar: "Glutamyl cysteine dipeptide suppresses ferritin expression and alleviates liver injury in iron-overload rat model." in: Biochimie, Vol. 115, pp. 203-11, 2015
Iii Colado-Velázquez, Mailloux-Salinas, Medina-Contreras, Cruz-Robles, Bravo: "Effect of Serenoa Repens on Oxidative Stress, Inflammatory and Growth Factors in Obese Wistar Rats with Benign Prostatic Hyperplasia." in: Phytotherapy research : PTR, Vol. 29, Issue 10, pp. 1525-31, 2015
Zou, Wen, Zheng, Xiao, Luo, Chen, Wang, Cheng, Xiang, Nie: "Metabolomic Study on the Preventive Effect of Patrinia scabiosaefolia Fisch on Multipathogen Induced Pelvic Inflammatory Disease in Rats." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 170792, 2015
Meireles, Marques, Norberto, Fernandes, Mateus, Rendeiro, Spencer, Faria, Calhau: "The impact of chronic blackberry intake on the neuroinflammatory status of rats fed a standard or high-fat diet." in: The Journal of nutritional biochemistry, Vol. 26, Issue 11, pp. 1166-73, 2015
El-Naga: "Apocynin protects against ethanol-induced gastric ulcer in rats by attenuating the upregulation of NADPH oxidases 1 and 4." in: Chemico-biological interactions, Vol. 242, pp. 317-26, 2015
Seif, Abdelwahed: "Vitamin D ameliorates hepatic ischemic/reperfusion injury in rats." in: Journal of physiology and biochemistry, Vol. 70, Issue 3, pp. 659-66, 2014
El-Naga, Ahmed, Abd Al Haleem: "Effects of indole-3-carbinol on clonidine-induced neurotoxicity in rats: Impact on oxidative stress, inflammation, apoptosis and monoamine levels." in: Neurotoxicology, Vol. 44, pp. 48-57, 2014
Kumar, Gupta, Nag, Srivastava, Saxena, Jha, Srinivasan: "Retinal neuroprotective effects of quercetin in streptozotocin-induced diabetic rats." in: Experimental eye research, Vol. 125, pp. 193-202, 2014
Srivastava, Neupane, Kumar, Kohli: "Nanoemulgel (NEG) of Ketoprofen with eugenol as oil phase for the treatment of ligature-induced experimental periodontitis in Wistar rats." in: Drug delivery, pp. 1-7, 2014
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