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anti-Human Insulin Receptor antibody for Enzyme Immunoassay

Recommended Insulin Receptor Antibody (supplied by: Log in to see )

Antigen
Insulin Receptor (INSR) Antibodies
  • 18402
  • 4932439J01Rik
  • CD220
  • cd220
  • CG18402
  • CTK-1
  • D630014A15Rik
  • DIHR
  • DILR
  • dInR
  • dINR
  • dinr
  • dInr
  • DInR
  • DInr
  • dInsR
  • dir
  • DIR
  • dIR
  • Dir-a
  • Dir-b
  • DIRbeta
  • dIRH
  • DIRH
  • Dmel\\CG18402
  • er10
  • HHF5
  • hhf5
  • Inr
  • INR
  • inr
  • Inr-alpha
  • Inr-beta
  • INS
  • INSR
  • InsR
  • insulin/insulin-like growth factor receptor
  • ir
  • IR
  • IR-A
  • ir-A
  • IR-B
  • l(3)05545
  • l(3)93Dj
  • l(3)er10
  • lnR
  • NV14476
Epitope
N-Term
31
29
21
20
15
10
9
7
6
6
6
6
5
5
5
5
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
265
81
57
11
8
5
4
2
1
1
1
1
1
1
1
Host
Rabbit
218
76
3
Clonality
Polyclonal
Conjugate
This Insulin Receptor antibody is un-conjugated
21
16
9
9
7
7
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
167
122
50
42
35
19
18
12
11
10
10
8
7
6
2
2
2
1
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Catalog No. ABIN359886
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008833851 ABIN358482 EIA Rabbit Ig pTyr1185 Log in to see Polyclonal 5
0.0008833851 ABIN126821 EIA WB Mouse IgG1 Beta Chain Log in to see 11B6
0.0008833851 ABIN126819 EIA WB Mouse IgG1 pTyr1322 Log in to see 21G12
0.0008833851 ABIN126820 EIA IHC (fro) WB Mouse IgG1 Beta Chain Log in to see 9H4
0.0008833851 ABIN1106085 EIA IHC (fro) WB Rabbit C-Term Log in to see Polyclonal
0.0008833851 ABIN2717512 EIA Rabbit IgG Log in to see Polyclonal

Top referenced anti-Insulin Receptor antibody for Enzyme Immunoassay

Similar anti-Insulin Receptor Antibodies

Application / Reactivity Human
Blocking Reagent (BR) 4 Antibodies
Dot Blot (DB) 18 Antibodies
ELISA 106 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 10 Antibodies
Functional Studies (Func) 19 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 8 Antibodies
Immunofluorescence (IF) 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 36 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 42 Antibodies
Immunoprecipitation (IP) 52 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Mass Cytometry (CyTOF) 2 Antibodies

General

Antigen Insulin Receptor (INSR) Antibodies
Epitope N-Term
(31), (29), (21), (20), (15), (10), (9), (7), (6), (6), (6), (6), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(265), (81), (57), (11), (8), (5), (4), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(218), (76), (3)
Clonality
Polyclonal
Conjugate This Insulin Receptor antibody is un-conjugated
(21), (16), (9), (9), (7), (7), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(167), (122), (50), (42), (35), (19), (18), (12), (11), (10), (10), (8), (7), (6), (2), (2), (2), (1)
Pubmed 5 references available
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Product Details anti-Insulin Receptor Antibody

Target Details Insulin Receptor Application Details Handling References for anti-Insulin Receptor Antibody (ABIN359886) Images
Specificity This antibody reacts to INSR (Insulin Receptor).
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human INSR.
Isotype Ig

Target Details Insulin Receptor

Product Details anti-Insulin Receptor Antibody Application Details Handling References for anti-Insulin Receptor Antibody (ABIN359886) Images back to top
Antigen
Alternative Name CD220 / INSR (INSR Antibody Abstract)
Background INSR is a receptor that binds insulin and has a tyrosine-protein kinase activity. Autophosphorylation activates the kinase activity. This Type I mebrane protein is composed of a tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand-binding domain, while the beta chains carry the kinase domain. After being transported from the endoplasmic reticulum to the Golgi apparatus, the single glycosylated precursor is further glycosylated and then cleaved, followed by its transport to the plasma membrane. Defects in INSR are the cause of insulin resistance of various forms, including mild insulin-resistant diabetes mellitus with acanthosis nigricans, minor physical abnormalities and sometimes polycystic ovaries. Insulin resistance associated with acanthosis nigricans, hirsutism and hyperandrogenism is referred to as insulin resistance type A. Defects in INSR are the cause of Rabson-Mendenhall syndrome, also known as Mendenhall syndrome. It is a severe insulin resistance syndrome characterized by insulin-resistant diabetes mellitus with pineal hyperplasia and somatic abnormalities. Typical features include coarse, senile-appearing facies, dental and skin abnormalities, abdominal distension, and phallic enlargement. Inheritance is autosomal recessive. Defects in INSR are the cause of leprechaunism, also known as Donohue syndrome. Leprechaunism represents the most severe form of insulin resistance syndrome, characterized by intrauterine and postnatal growth retardation and death in early infancy. Inheritance is autosomal recessive. Defects in INSR may be associated with noninsulin-dependent diabetes mellitus.Synonyms: Insulin Receptor
Gene ID 3643, 9606
UniProt P06213
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways NF-kappaB Signaling, RTK Signaling, AMPK Signaling

Application Details

Product Details anti-Insulin Receptor Antibody Target Details Insulin Receptor Handling References for anti-Insulin Receptor Antibody (ABIN359886) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Restrictions For Research Use only

Handling

Product Details anti-Insulin Receptor Antibody Target Details Insulin Receptor Application Details References for anti-Insulin Receptor Antibody (ABIN359886) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-Insulin Receptor Antibody (ABIN359886)

Product Details anti-Insulin Receptor Antibody Target Details Insulin Receptor Application Details Handling Images back to top
Background publications

George, Johansen, Soos, Mortensen, Gammeltoft, Saudek, Siddle, Hansen, ORahilly: "Deletion of V335 from the L2 domain of the insulin receptor results in a conformationally abnormal receptor that is unable to bind insulin and causes Donohue's syndrome in a human subject." in: Endocrinology, Vol. 144, Issue 2, pp. 631-7, 2003

Longo, Wang, Smith, Langley, DiMeglio, Giannella-Neto: "Genotype-phenotype correlation in inherited severe insulin resistance." in: Human molecular genetics, Vol. 11, Issue 12, pp. 1465-75, 2002

Hamer, Foti, Emkey, Cordier-Bussat, Philippe, De Meyts, Maeder, Kahn, Carpentier: "An arginine to cysteine(252) mutation in insulin receptors from a patient with severe insulin resistance inhibits receptor internalisation but preserves signalling events." in: Diabetologia, Vol. 45, Issue 5, pp. 657-67, 2002

Osawa, Nishimiya, Ochi, Niiya, Onuma, Kitamuro, Kaino, Kida, Makino: "Identification of novel C253Y missense and Y864X nonsense mutations in the insulin receptor gene in type A insulin-resistant patients." in: Clinical genetics, Vol. 59, Issue 3, pp. 194-7, 2001

Riqué, Nogués, Ibàñez, Marcos, Ferragut, Carrascosa, Potau: "Identification of three novel mutations in the insulin receptor gene in type A insulin resistant patients." in: Clinical genetics, Vol. 57, Issue 1, pp. 67-9, 2000

Images

Product Details anti-Insulin Receptor Antibody Target Details Insulin Receptor Application Details Handling References for anti-Insulin Receptor Antibody (ABIN359886) back to top
Supplier Images
 image for anti-Insulin Receptor antibody (INSR) (N-Term) (ABIN359886) anti-Insulin Receptor (INSR) (N-Term) antibody
 image for anti-Insulin Receptor antibody (INSR) (N-Term) (ABIN359886) anti-Insulin Receptor (INSR) (N-Term) antibody (Image 2)