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|Application / Reactivity||Human|
|Blocking Reagent (BR)||4 Antibodies|
|Dot Blot (DB)||18 Antibodies|
|Enzyme Immunoassay (EIA)||7 Antibodies|
|Flow Cytometry (FACS)||10 Antibodies|
|Functional Studies (Func)||19 Antibodies|
|Immunochromatography (IC)||2 Antibodies|
|Immunocytochemistry (ICC)||8 Antibodies|
|Immunofluorescence (IF)||9 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||37 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||6 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||43 Antibodies|
|Immunoprecipitation (IP)||52 Antibodies|
|Intracellular Flow Cytometry (ICFC)||2 Antibodies|
|Mass Cytometry (CyTOF)||2 Antibodies|
|Antigen||Insulin Receptor (INSR) Antibodies|
|Conjugate||This Insulin Receptor antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-Insulin Receptor AntibodyTarget Details Insulin Receptor Application Details Handling References for anti-Insulin Receptor Antibody (ABIN359886) Images
|Specificity||This antibody reacts to INSR (Insulin Receptor).|
|Purification||Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human INSR.|
Target Details Insulin ReceptorProduct Details anti-Insulin Receptor Antibody Application Details Handling References for anti-Insulin Receptor Antibody (ABIN359886) Images back to top
|Alternative Name||CD220 / INSR (INSR Antibody Abstract)|
|Background||INSR is a receptor that binds insulin and has a tyrosine-protein kinase activity. Autophosphorylation activates the kinase activity. This Type I mebrane protein is composed of a tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand-binding domain, while the beta chains carry the kinase domain. After being transported from the endoplasmic reticulum to the Golgi apparatus, the single glycosylated precursor is further glycosylated and then cleaved, followed by its transport to the plasma membrane. Defects in INSR are the cause of insulin resistance of various forms, including mild insulin-resistant diabetes mellitus with acanthosis nigricans, minor physical abnormalities and sometimes polycystic ovaries. Insulin resistance associated with acanthosis nigricans, hirsutism and hyperandrogenism is referred to as insulin resistance type A. Defects in INSR are the cause of Rabson-Mendenhall syndrome, also known as Mendenhall syndrome. It is a severe insulin resistance syndrome characterized by insulin-resistant diabetes mellitus with pineal hyperplasia and somatic abnormalities. Typical features include coarse, senile-appearing facies, dental and skin abnormalities, abdominal distension, and phallic enlargement. Inheritance is autosomal recessive. Defects in INSR are the cause of leprechaunism, also known as Donohue syndrome. Leprechaunism represents the most severe form of insulin resistance syndrome, characterized by intrauterine and postnatal growth retardation and death in early infancy. Inheritance is autosomal recessive. Defects in INSR may be associated with noninsulin-dependent diabetes mellitus.Synonyms: Insulin Receptor|
|Gene ID||3643, 9606|
|Research Area||CD Antigens, Surface Receptors of Immune Cells|
|Pathways||NF-kappaB Signaling, RTK Signaling, AMPK Signaling|
Application DetailsProduct Details anti-Insulin Receptor Antibody Target Details Insulin Receptor Handling References for anti-Insulin Receptor Antibody (ABIN359886) Images back to top
|Application Notes||ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-Insulin Receptor Antibody Target Details Insulin Receptor Application Details References for anti-Insulin Receptor Antibody (ABIN359886) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-Insulin Receptor Antibody (ABIN359886)Product Details anti-Insulin Receptor Antibody Target Details Insulin Receptor Application Details Handling Images back to top
George, Johansen, Soos et al.: "Deletion of V335 from the L2 domain of the insulin receptor results in a conformationally abnormal receptor that is unable to bind insulin and causes Donohue's syndrome in a human subject." in: Endocrinology, Vol. 144, Issue 2, pp. 631-7, 2003 (PubMed).
Hamer, Foti, Emkey et al.: "An arginine to cysteine(252) mutation in insulin receptors from a patient with severe insulin resistance inhibits receptor internalisation but preserves signalling events." in: Diabetologia, Vol. 45, Issue 5, pp. 657-67, 2002 (PubMed).
Longo, Wang, Smith et al.: "Genotype-phenotype correlation in inherited severe insulin resistance." in: Human molecular genetics, Vol. 11, Issue 12, pp. 1465-75, 2002 (PubMed).
Osawa, Nishimiya, Ochi et al.: "Identification of novel C253Y missense and Y864X nonsense mutations in the insulin receptor gene in type A insulin-resistant patients." in: Clinical genetics, Vol. 59, Issue 3, pp. 194-7, 2001 (PubMed).
Riqué, Nogués, Ibàñez et al.: "Identification of three novel mutations in the insulin receptor gene in type A insulin resistant patients." in: Clinical genetics, Vol. 57, Issue 1, pp. 67-9, 2000 (PubMed).
ImagesProduct Details anti-Insulin Receptor Antibody Target Details Insulin Receptor Application Details Handling References for anti-Insulin Receptor Antibody (ABIN359886) back to top