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anti-Human TNFRSF1A antibody for Immunohistochemistry (Frozen Sections)

Recommended TNFRSF1A Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) Antibodies
  • cd120a
  • CD120a
  • fpf
  • FPF
  • MS5
  • p55
  • p55-R
  • p55-r
  • p60
  • TBP1
  • tbp1
  • TNF-alphaR1
  • TNF-R
  • tnf-r
  • TNF-R-I
  • TNF-R1
  • TNF-R55
  • TNFalpha-R1
  • tnfar
  • TNFAR
  • Tnfr-2
  • tnfr1
  • Tnfr1
  • TNFR1
  • TNFR1-d2
  • tnfr55
  • TNFR55
  • TNFR60
  • tnfr60
  • TNFR I
  • TNFRI
  • TNFRp55
  • TNFRSF1A
  • wu:fj65b10
  • xtnfr1
  • zgc:85914
Reactivity
Human
323
162
81
38
33
31
27
14
9
8
8
7
6
4
4
2
2
1
1
Host
Mouse
257
109
18
17
16
14
1
Clonality
Monoclonal
Conjugate
This TNFRSF1A antibody is un-conjugated
32
30
29
13
10
9
5
5
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
259
166
150
114
102
66
52
34
31
23
22
12
11
10
7
2
2
2
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN111937
$ 511.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008829923 ABIN111938 FACS IHC (fro) WB Biotin Mouse IgG1 Log in to see htr9 7
0.0008829923 ABIN1105786 EIA IHC (fro) IP WB Mouse IgG1 Log in to see MR1-2 3
0.0008829923 ABIN1105795 EIA IHC (fro) FACS WB Biotin Mouse IgG2a Log in to see H398 5
0.0008829923 ABIN111939 Func FACS IHC (fro) WB Mouse IgG1 Log in to see htr9 6
0.0008829923 ABIN116870 EIA IHC (fro) IP WB Rabbit Log in to see Polyclonal 4
0.0008829923 ABIN1105792 Func EIA IHC (fro) FACS IP WB Mouse IgG2a Log in to see H398 5
0.0008829923 ABIN1105793 EIA IHC (fro) Biotin Mouse IgG1 Log in to see MR1-2 2
0.0008829923 ABIN1105798 EIA IHC (fro) IP WB Biotin Mouse Log in to see MR1-2 2
0.0008829923 ABIN116869 EIA IHC (fro) IP WB Rabbit Log in to see Polyclonal 4
0.0008829923 ABIN602488 IHC (fro) FACS IP ELISA WB Mouse IgG1 Log in to see MR1-2
0.0008829923 ABIN1105787 Func EIA IHC (fro) Mouse IgG1 Log in to see MR1-2 1
0.0008829923 ABIN2478295 Func IHC (fro) FACS IP Mouse IgG2a Log in to see H398 2
0.0008829923 ABIN299012 Func FACS IHC (fro) IP Mouse IgG2a Log in to see H398
0.0008829923 ABIN204876 CyTox FACS IHC (fro) Mouse IgG2a Log in to see 4-12
0.0008829923 ABIN2191869 IA IHC (fro) FACS WB Biotin Mouse Log in to see MR1-2 1
0.0008829923 ABIN1077710 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 60-236 Log in to see Polyclonal
0.0008829923 ABIN2191868 Agon CC IA IHC (fro) FACS Mouse Log in to see MR1-2 2
0.0008829923 ABIN1838187 IHC (fro) FACS WB Biotin Mouse IgG2a Log in to see H398-Biotinylated
0.0008829923 ABIN1838192 IHC (fro) FACS IP WB Mouse IgG1 Log in to see MR1-2
0.0008829923 ABIN1838189 IHC (fro) FACS IP WB Biotin Mouse IgG1 Log in to see 80M2-Biotinylated

Top referenced anti-TNFRSF1A antibody for Immunohistochemistry (Frozen Sections)

Similar anti-TNFRSF1A Antibodies

Application / Reactivity Human
Agonist (Agon) 1 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
Cell Culture (CC) 2 Antibodies
Cytotoxicity Test (CyTox) 1 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 111 Antibodies
Enzyme Immunoassay (EIA) 18 Antibodies
Flow Cytometry (FACS) 122 Antibodies
Functional Studies (Func) 14 Antibodies
Immunoassay (IA) 4 Antibodies
Immunocytochemistry (ICC) 28 Antibodies
Immunofluorescence (IF) 35 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 94 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 44 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 60 Antibodies

General

Antigen Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) Antibodies
Reactivity Human
(323), (162), (81), (38), (33), (31), (27), (14), (9), (8), (8), (7), (6), (4), (4), (2), (2), (1), (1)
Host Mouse
(257), (109), (18), (17), (16), (14), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TNFRSF1A antibody is un-conjugated
(32), (30), (29), (13), (10), (9), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(259), (166), (150), (114), (102), (66), (52), (34), (31), (23), (22), (12), (11), (10), (7), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-TNFRSF1A Antibody

Target Details TNFRSF1A Application Details Handling References for anti-TNFRSF1A Antibody (ABIN111937) Images
Purification Immunoaffinity Chromatography
Immunogen Partially purified preparation of TNF binding protein from HL-60 cells.
Clone Htr9
Isotype IgG1

Target Details TNFRSF1A

Product Details anti-TNFRSF1A Antibody Application Details Handling References for anti-TNFRSF1A Antibody (ABIN111937) Images back to top
Antigen
Alternative Name CD120a / TNFR1 (TNFRSF1A Antibody Abstract)
Background Tumor Necrosis Factor (TNF) is a cytokine whose function is mediated through two distinct cell surface receptors (TNF Receptor I and TNF Receptor II) that are included in the TNF Receptor superfamily along with FAS antigen and CD40. TNF Receptors I and II are 55 and 75 kDa members, respectively, of a family of cell surface molecules including nerve growth factor receptor, Fas/Apo1, CD30, OX40, and 41BB, which are characterized by cysteine rich motifs in the extracellular domain. While TNF Receptor I and TNF Receptor II share 28 % sequence homology in the extracellular domains, their intracellular domains lack sequence homology, suggesting that they differ in their internal signal transduction pathways. TNF Receptor I contains an approximately 80 amino acid death domain near its carboxy terminus capable of transmitting an apoptotic signal through its interaction with TRADD (TNF Receptor I associated death domain protein), and subsequent interactions with FADD. TNF Receptor I can also activate the transcription factor NFkB via TRAF2 (TNF Receptor associated factor 2). The cytoplasmic domain of TNF Receptor I can directly interact with Jak kinase, thereby activating the JAK/STAT signal transduction cascade. TNF Receptor I is expressed by virtually all nucleated mammalian cells, including hepatocytes, monocytes and neutrophils, cardiac muscle cells, endothelial cells, and CD34 + hematopoietic progenitors. Both TNF alpha and TNF beta bind to TNF Receptor I.Synonyms: TNF-R1, TNF-RI, TNFR-I, Tnfrsf1a, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor type I, p55, p60
Gene ID 9606
UniProt P19438
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-TNFRSF1A Antibody Target Details TNFRSF1A Handling References for anti-TNFRSF1A Antibody (ABIN111937) Images back to top
Application Notes htr 9 is useful for studying biological effects of TNF-receptor p55 in vitro. The antibodyinhibits the binding of radiolabelled TNF to human cells expressing the p55 TNF receptor. Inorder to obtain complete inhibition of TNF binding to the cell surface, 10 μg/mL of htr-9 arerequired. htr 9 itself may have an agonistic effect in assays measuring cytotoxicity,
Protocol Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS
Restrictions For Research Use only

Handling

Product Details anti-TNFRSF1A Antibody Target Details TNFRSF1A Application Details References for anti-TNFRSF1A Antibody (ABIN111937) Images back to top
Reconstitution Restore with 0.5 mL distilled water.
Concentration 0.4 mg/mL
Buffer PBS, pH 7.2, 0.05 % Kathon CG, 5 mg/mL BSA
Preservative Kathon CG
Precaution of Use This product contains Kathon CG a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage 4 °C
Storage Comment Store the antibody undiluted at 4-8 °C for one month or at -20 °C for longer.

References for anti-TNFRSF1A Antibody (ABIN111937)

Product Details anti-TNFRSF1A Antibody Target Details TNFRSF1A Application Details Handling Images back to top
Background publications

Gehr, Gentz, Brockhaus, Loetscher, Lesslauer: "Both tumor necrosis factor receptor types mediate proliferative signals in human mononuclear cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 3, pp. 911-7, 1992

Vandenabeele, Declercq, Vercammen, Van de Craen, Grooten, Loetscher, Brockhaus, Lesslauer, Fiers: "Functional characterization of the human tumor necrosis factor receptor p75 in a transfected rat/mouse T cell hybridoma." in: The Journal of experimental medicine, Vol. 176, Issue 4, pp. 1015-24, 1992

Ryffel, Brockhaus, Dürmüller, Gudat: "Tumor necrosis factor receptors in lymphoid tissues and lymphomas. Source and site of action of tumor necrosis factor alpha." in: The American journal of pathology, Vol. 139, Issue 1, pp. 7-15, 1991

Hohmann, Brockhaus, Baeuerle, Remy, Kolbeck, van Loon: "Expression of the types A and B tumor necrosis factor (TNF) receptors is independently regulated, and both receptors mediate activation of the transcription factor NF-kappa B. TNF alpha is not needed for induction of a biological effect via TNF receptors." in: The Journal of biological chemistry, Vol. 265, Issue 36, pp. 22409-17, 1991

Brockhaus, Schoenfeld, Schlaeger, Hunziker, Lesslauer, Loetscher: "Identification of two types of tumor necrosis factor receptors on human cell lines by monoclonal antibodies." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 8, pp. 3127-31, 1990

Espevik, Brockhaus, Loetscher, Nonstad, Shalaby: "Characterization of binding and biological effects of monoclonal antibodies against a human tumor necrosis factor receptor." in: The Journal of experimental medicine, Vol. 171, Issue 2, pp. 415-26, 1990

Shalaby, Sundan, Loetscher, Brockhaus, Lesslauer, Espevik: "Binding and regulation of cellular functions by monoclonal antibodies against human tumor necrosis factor receptors." in: The Journal of experimental medicine, Vol. 172, Issue 5, pp. 1517-20, 1990