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anti-Mouse (Murine) TNFRSF1A antibody for Blocking Reagent

Recommended TNFRSF1A Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) Antibodies
  • cd120a
  • CD120a
  • fpf
  • FPF
  • MS5
  • p55
  • p55-R
  • p55-r
  • p60
  • TBP1
  • tbp1
  • TNF-alphaR1
  • TNF-R
  • tnf-r
  • TNF-R-I
  • TNF-R1
  • TNF-R55
  • TNFalpha-R1
  • tnfar
  • TNFAR
  • Tnfr-2
  • tnfr1
  • Tnfr1
  • TNFR1
  • TNFR1-d2
  • tnfr55
  • TNFR55
  • TNFR60
  • tnfr60
  • TNFR I
  • TNFRI
  • TNFRp55
  • TNFRSF1A
  • wu:fj65b10
  • xtnfr1
  • zgc:85914
Reactivity
Mouse (Murine)
291
146
79
36
32
29
26
13
9
7
7
6
5
4
4
2
1
1
1
Host
Armenian Hamster
253
82
17
16
13
4
1
Clonality
Monoclonal
Conjugate
This TNFRSF1A antibody is un-conjugated
31
28
27
13
9
9
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), Functional Studies (Func), Neutralization (Neut)
240
136
125
98
97
44
34
33
31
23
21
11
10
9
7
2
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN1176998
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Similar anti-TNFRSF1A Antibodies

Application / Reactivity Mouse (Murine)
Blocking Reagent (BR) 1 Antibodies
ELISA 20 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 34 Antibodies
Functional Studies (Func) 8 Antibodies
Immunoassay (IA) 3 Antibodies
Immunocytochemistry (ICC) 21 Antibodies
Immunofluorescence (IF) 21 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 45 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 20 Antibodies
Immunoprecipitation (IP) 62 Antibodies
Intracellular Flow Cytometry (ICFC) 1 Antibodies
Mass Cytometry (CyTOF) 2 Antibodies
Neutralization (Neut) 4 Antibodies

General

Antigen Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) Antibodies
Reactivity Mouse (Murine)
(291), (146), (79), (36), (32), (29), (26), (13), (9), (7), (7), (6), (5), (4), (4), (2), (1), (1), (1)
Host Armenian Hamster
(253), (82), (17), (16), (13), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TNFRSF1A antibody is un-conjugated
(31), (28), (27), (13), (9), (9), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Functional Studies (Func), Neutralization (Neut)
(240), (136), (125), (98), (97), (44), (34), (33), (31), (23), (21), (11), (10), (9), (7), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-TNFRSF1A Antibody

Target Details TNFRSF1A Application Details Handling Images
Characteristics Neutralization Activity: 50% Neutralization (ND50) at 0.25 - 1.0 myg/mL
>95% Neutralization at 2.0 - 8.0 myg/ml
Purification Purified from tissue culture supernatant or ascites by affinity chromatography
Sterility 0.2 μm filtered
Endotoxin Level ≤0.01 ng/µg
Immunogen Mouse purified, soluble, extracellular domain type I TNFR
Clone 55R-170
Isotype IgG1 kappa

Target Details TNFRSF1A

Product Details anti-TNFRSF1A Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD120a (TNFRSF1A Antibody Abstract)
Background The 55R-170 antibody reacts with the extracellular region of mouse CD120a which is also known as the 55 kDa receptor for the mouse cytokines, tumor necrosis factor (TNF) and lymphotoxin-alpha (LT-alpha, formerly known as TNF-beta). This receptor, referred to as the p55 or Type I Tumor Necrosis Factor Receptor (TNFR) is expressed by a variety of different cell types. In addition, the 55R-170 can bind to a soluble, truncated form of the mouse Type I TNFR. The 55R-170 antibody does not recognize the 75 kDa (p75) Type II TNFR (aka, CD120b). The binding of [125]I-human TNF (that can bind to p55 but not p75 TNFR expressed by mouse L929 cells), is blocked by 55R-170. Moreover, the 55R-170 antibody neutralizes the cytolytic activity of recombinant mouse and human TNF protein on L929 cell populations that express p55 (and p75) TNFR. When aggregated or crosslinked by another antibody, the 55R-170 antibody can have agonist activity, i.e., can mimic the cytolytic activity of TNF. In vivo administration of the 55R-170 antibody blocks endotoxin-induced lethal shock in mice. Skin necrosis induced by the in vivo administration of TNF is reportedly blocked by 55R-170 antibody. The immunogen used to generate the 55R-170 hybridoma was the purified, soluble, extracellular domain of the mouse Type I TNFR.
Research Area Surface Receptors of Immune Cells
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-TNFRSF1A Antibody Target Details TNFRSF1A Handling Images back to top
Application Notes In vitro neutralization: The NA/LE™ format of this antibody is useful for neutralization of mouse TNF activity. Since applications vary, titrate the reagent to obtain optimal results. Assay Type: Cytolysis using 700 pg/ml mouse TNF (Cat No. 554589) measured by crystal violet staining Indicator cell: L929 at 7.5 x 10^5 cells/ml and Preincubation of antibody and ligand = 60 minutes Please note that the ratio of antibody to TNF required to neutralize mouse TNF is 200- 300 fold higher than that required to neutralize human TNF.
Immunoprecipitation: The 55R-170 antibody has been reported to be useful for the immunoprecipitation of p55 TNFR from mouse L929 cells. Please note that this application is not routinely tested at BD Biosciences.
Restrictions For Research Use only

Handling

Product Details anti-TNFRSF1A Antibody Target Details TNFRSF1A Application Details Images back to top
Format Liquid
Concentration 1.0 mg/ml
Buffer Aqueous buffered solution
Preservative Azide free
Storage 4 °C