|Application / Reactivity||Rabbit||Human|
|ELISA||1 Antibodies||110 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||18 Antibodies|
|Flow Cytometry (FACS)||7 Antibodies||93 Antibodies|
|Functional Studies (Func)||1 Antibodies|
|Immunocytochemistry (ICC)||18 Antibodies|
|Immunofluorescence (IF)||18 Antibodies|
|Immunohistochemistry (IHC)||23 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||5 Antibodies|
|Immunoprecipitation (IP)||21 Antibodies|
|Radioimmunoassay (RIA)||2 Antibodies|
|Western Blotting (WB)||29 Antibodies|
|Agonist (Agon)||1 Antibodies|
|Blocking Antibody (Inhibition)||1 Antibodies|
|Cell Culture (CC)||2 Antibodies|
|Cytotoxicity Test (CyTox)||1 Antibodies|
|Dot Blot (DB)||1 Antibodies|
|Antigen||Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) Antibodies|
|Reactivity||Human, Rabbit Alternatives|
|Conjugate||This TNFRSF1A antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Radioimmunoassay (RIA)
|4 references available|
|Supplier||Log in to see|
Product Details anti-TNFRSF1A AntibodyTarget Details TNFRSF1A Application Details Handling References for anti-TNFRSF1A Antibody (ABIN118847) Images
|Purification||Affinity chromatography on Protein A|
|Immunogen||Purified human tumor Necrosis Factor Receptor type 1. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSO myeloma cell line.|
Target Details TNFRSF1AProduct Details anti-TNFRSF1A Antibody Application Details Handling References for anti-TNFRSF1A Antibody (ABIN118847) Images back to top
|Alternative Name||CD120a / TNFR1 (TNFRSF1A Antibody Abstract)|
|Background||Tumor Necrosis Factor (TNF) is a cytokine whose function is mediated through two distinct cell surface receptors (TNF Receptor I and TNF Receptor II) that are included in the TNF Receptor superfamily along with FAS antigen and CD40. TNF Receptors I and II are 55 and 75 kDa members, respectively, of a family of cell surface molecules including nerve growth factor receptor, Fas/Apo1, CD30, OX40, and 41BB, which are characterized by cysteine rich motifs in the extracellular domain. While TNF Receptor I and TNF Receptor II share 28 % sequence homology in the extracellular domains, their intracellular domains lack sequence homology, suggesting that they differ in their internal signal transduction pathways. TNF Receptor I contains an approximately 80 amino acid death domain near its carboxy terminus capable of transmitting an apoptotic signal through its interaction with TRADD (TNF Receptor I associated death domain protein), and subsequent interactions with FADD. TNF Receptor I can also activate the transcription factor NFkB via TRAF2 (TNF Receptor associated factor 2). The cytoplasmic domain of TNF Receptor I can directly interact with Jak kinase, thereby activating the JAK/STAT signal transduction cascade. TNF Receptor I is expressed by virtually all nucleated mammalian cells, including hepatocytes, monocytes and neutrophils, cardiac muscle cells, endothelial cells, and CD34 + hematopoietic progenitors. Both TNF alpha and TNF beta bind to TNF Receptor I.Synonyms: TNF-R1, TNF-RI, TNFR-I, Tnfrsf1a, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor type I, p55, p60|
|Research Area||CD Antigens, Surface Receptors of Immune Cells|
|Pathways||NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-TNFRSF1A Antibody Target Details TNFRSF1A Handling References for anti-TNFRSF1A Antibody (ABIN118847) Images back to top
Flow Cytometry. ELISA. Radioimmunoassay. Removal of sodium azide is recommended prior to use in functional assays. Clone H398 may be used to detect high levels of TNFR1, in western blotting under reducing conditions, such as recombinant material, but it is not suitable for detection of TNFR1 in normal cells in this application.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-TNFRSF1A Antibody Target Details TNFRSF1A Application Details References for anti-TNFRSF1A Antibody (ABIN118847) Images back to top
|Buffer||PBS, pH 7.4 containing 0.09 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-TNFRSF1A Antibody (ABIN118847)Product Details anti-TNFRSF1A Antibody Target Details TNFRSF1A Application Details Handling Images back to top
Kohrgruber, Halanek, Gröger et al.: "Survival, maturation, and function of CD11c- and CD11c+ peripheral blood dendritic cells are differentially regulated by cytokines." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 6, pp. 3250-9, 1999 (PubMed).
Dri, Haas, Cramer et al.: "Role of the 75-kDa TNF receptor in TNF-induced activation of neutrophil respiratory burst." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 1, pp. 460-6, 1999 (PubMed).
Menegazzi, Cramer, Patriarca et al.: "Evidence that tumor necrosis factor alpha (TNF)-induced activation of neutrophil respiratory burst on biologic surfaces is mediated by the p55 TNF receptor." in: Blood, Vol. 84, Issue 1, pp. 287-93, 1994 (PubMed).
Thoma, Grell, Pfizenmaier et al.: "Identification of a 60-kD tumor necrosis factor (TNF) receptor as the major signal transducing component in TNF responses." in: The Journal of experimental medicine, Vol. 172, Issue 4, pp. 1019-23, 1990 (PubMed).