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anti-Human TNFSF11 antibody for Functional Studies

Recommended TNFSF11 Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) Antibodies
  • BTF3L1
  • CD254
  • hRANKL2
  • HUMBTFB
  • Ly109l
  • OCIF
  • ODF
  • OPG
  • Opg
  • OPGL
  • OPTB2
  • RANKL
  • sOdf
  • TNFRSF11B
  • TNFSF11
  • TRANCE
  • Trance
Reactivity
Human
169
117
40
1
1
1
1
Host
Rabbit
163
47
29
7
1
Clonality
Polyclonal
Conjugate
This TNFSF11 antibody is un-conjugated
26
12
6
6
5
2
2
2
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
173
107
46
44
28
22
21
12
10
10
9
5
4
3
1
1
1
1
Supplier
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Catalog No. ABIN116282
$ 368.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008758766 ABIN116281 EIA Func IHC (p) WB Rabbit Log in to see Polyclonal 1
0.0008758766 ABIN3036983 Func FACS Mouse IgG1 AA 95-281 Log in to see

Top referenced anti-TNFSF11 antibody for Functional Studies

Similar anti-TNFSF11 Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 4 Antibodies
ELISA 79 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 10 Antibodies
Flow Cytometry (FACS) 18 Antibodies
Functional Studies (Func) 3 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 9 Antibodies
Immunofluorescence (IF) 21 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 28 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 23 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Neutralization (Neut) 1 Antibodies

General

Antigen Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11) Antibodies
Reactivity Human
(169), (117), (40), (1), (1), (1), (1)
Host Rabbit
(163), (47), (29), (7), (1)
Clonality
Polyclonal
Conjugate This TNFSF11 antibody is un-conjugated
(26), (12), (6), (6), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(173), (107), (46), (44), (28), (22), (21), (12), (10), (10), (9), (5), (4), (3), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-TNFSF11 Antibody

Target Details TNFSF11 Application Details Handling References for anti-TNFSF11 Antibody (ABIN116282) Images
Specificity This antibody reacts with soluble RANK Ligand.
Purification Affinity Chromatography.
Immunogen Highly pure (>98%) recombinant Human sRANKL.

Target Details TNFSF11

Product Details anti-TNFSF11 Antibody Application Details Handling References for anti-TNFSF11 Antibody (ABIN116282) Images back to top
Antigen
Alternative Name CD254 / RANKL (TNFSF11 Antibody Abstract)
Background RANKL is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. There are three isoforms of RANKL. Human RANKL is a soluble 20 kDa polypeptide, comprising the TNF homologous region of RANKL (176 amino acid residues). This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor associated factor (TRAF) 6, which indicated that this protein may have a role in the regulation of cell apoptosis. RANKL deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis.Synonyms: ODF, OPGL, Osteoclast differentiation factor, Osteoprotegerin ligand, RANK Ligand, RANKL, Receptor activator of nuclear factor kappa B ligand, TNF-related activation-induced cytokine, TNFSF11, TRANCE, Tumor necrosis factor ligand superfamily member 11
Gene ID 8600
NCBI Accession NP_003692
UniProt O14788
Pathways NF-kappaB Signaling

Application Details

Product Details anti-TNFSF11 Antibody Target Details TNFSF11 Handling References for anti-TNFSF11 Antibody (ABIN116282) Images back to top
Application Notes Neutralization. Immunohistochemistry on Formalin-Fixed, Parrafin-embedded Sections: 0.25 μg/mL withan overnight incubation at 4 °C. Staining of Human metastatic carcinoma of lymph nodefrom breast tissue. An HRP-labeled polymer detection system was used with DABchromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer isrecommended. Sandwich ELISA: To detect Human sRANKL by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of at least 0.5-2.0 μg/mL of this antibody is required. This antigenaffinity purified antibody, in conjunction with Biotinylated anti-Human sRANKL (PP1086B1or PP1086B2) as a Detection antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant Human sRANKL. Western blot: To detect Human sRANKL by Western Blot analysis this antibody can be usedat a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary
Restrictions For Research Use only

Handling

Product Details anti-TNFSF11 Antibody Target Details TNFSF11 Application Details References for anti-TNFSF11 Antibody (ABIN116282) Images back to top
Reconstitution Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer PBS, pH 7.2 without preservatives
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

References for anti-TNFSF11 Antibody (ABIN116282)

Product Details anti-TNFSF11 Antibody Target Details TNFSF11 Application Details Handling Images back to top
Background publications

Grimaud, Soubigou, Couillaud, Coipeau, Moreau, Passuti, Gouin, Redini, Heymann: "Receptor activator of nuclear factor kappaB ligand (RANKL)/osteoprotegerin (OPG) ratio is increased in severe osteolysis." in: The American journal of pathology, Vol. 163, Issue 5, pp. 2021-31, 2003

Zhang, Dai, Qi, Lin, Smith, Strayhorn, Mizokami, Fu, Westman, Keller: "Osteoprotegerin inhibits prostate cancer-induced osteoclastogenesis and prevents prostate tumor growth in the bone." in: The Journal of clinical investigation, Vol. 107, Issue 10, pp. 1235-44, 2001