anti-Human Tumor Necrosis Factor antibody for ELISA

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Reactivity
Baboon, Human, Pig (Porcine), Primate
1229
445
358
145
136
119
119
61
55
41
29
18
12
12
8
7
6
4
4
4
4
2
1
1
1
1
Host
Mouse
928
490
182
48
10
9
7
6
3
2
2
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is conjugated to Biotin
164
97
71
50
32
30
27
26
25
25
25
25
25
25
24
24
24
20
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), ELISA
869
853
599
311
273
230
201
180
137
118
98
70
27
26
25
22
20
16
14
8
7
6
6
6
6
5
5
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN263069
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
10.216906 ABIN263068 ELISA FACS ICC IF IHC IHC (fro) IHC (p) Mouse IgG1 kappa Log in to see MAb11 1
10.216906 ABIN563205 IHC (p) ELISA WB Mouse IgG1 kappa AA 1-233, full length Log in to see M1-C4 1
10.216906 ABIN2668485 ELISA Mouse IgG2a Log in to see
10.216906 ABIN563204 ELISA WB Mouse IgG1 kappa AA 1-233, full length Log in to see 1C3-A1-F4
10.216906 ABIN563206 ELISA WB Mouse IgG1 kappa AA 1-233, full length Log in to see M2-E3
1 ABIN1997163 CC ELISA FACS Mouse IgG1 AA 77-233 Log in to see 9A7A5G9 1
1 ABIN1043796 Neut IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
1 ABIN1582141 IHC ELISA WB Rabbit Middle Region Log in to see Polyclonal 5
1 ABIN1582064 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal 2
1 ABIN1997173 ELISA WB Rabbit IgG AA 77-233 Log in to see Polyclonal 1
1 ABIN1724680 ELISA WB Mouse IgG1 Log in to see 2A9B9 3
1 ABIN3187695 ELISA IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN3187296 ELISA IF IHC Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN153474 IHC (p) IHC ELISA WB Rabbit Log in to see Polyclonal 8
1 ABIN1176946 Neut ELISA Mouse IgG1 Log in to see MAb1 2
1 ABIN1533583 IF IHC ELISA Rabbit IgG Log in to see Polyclonal
1 ABIN1582139 IP IHC ELISA WB Mouse IgG Log in to see 2
1 ABIN1997169 ELISA Rabbit IgG AA 77-233 Log in to see 211 1
1 ABIN305730 FACS IHC (fro) IHC (p) ELISA WB Mouse IgG1 Log in to see 52B83
1 ABIN1177286 Neut ELISA Mouse IgG2a, kappa Log in to see MABTNF-A5 3

Top referenced anti-Tumor Necrosis Factor antibody for ELISA

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Primate Pig (Porcine)
Blocking Reagent (BR) 1 Antibodies 1 Antibodies
ELISA 7 Antibodies 42 Antibodies
Flow Cytometry (FACS) 9 Antibodies 22 Antibodies
Functional Studies (Func) 2 Antibodies 18 Antibodies
Immunocytochemistry (ICC) 6 Antibodies 19 Antibodies
Immunofluorescence (IF) 5 Antibodies 6 Antibodies
Immunohistochemistry (IHC) 4 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 6 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Neutralization (Neut) 1 Antibodies
Radioimmunoassay (RIA) 1 Antibodies
Western Blotting (WB) 4 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Baboon, Human, Pig (Porcine), Primate
(1229), (445), (358), (145), (136), (119), (119), (61), (55), (41), (29), (18), (12), (12), (8), (7), (6), (4), (4), (4), (4), (2), (1), (1), (1), (1)
Host Mouse
(928), (490), (182), (48), (10), (9), (7), (6), (3), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is conjugated to Biotin
(164), (97), (71), (50), (32), (30), (27), (26), (25), (25), (25), (25), (25), (25), (24), (24), (24), (20), (13), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), ELISA
(869), (853), (599), (311), (273), (230), (201), (180), (137), (118), (98), (70), (27), (26), (25), (22), (20), (16), (14), (8), (7), (6), (6), (6), (6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN263069) Images
Specificity TNF- alpha (MAb11)
Purification Protein A or G purified
Immunogen E. coli-expressed, recombinant human TNF- alpha
Clone MAb11
Isotype IgG1 kappa

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN263069) Images back to top
Antigen
Alternative Name TNF-alpha (TNF Antibody Abstract)
Background Gene Symbol: TNF
Gene ID 7124
Research Area Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN263069) Images back to top
Application Notes Flow Cytometry 1:10-1:1000, ELISA 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:10-1:2000, Sandwich ELISAThe purified MAb11 has been tested by blocking fluorochrome conjugated MAb11 for intracellular cytokine staining. In order to obtain complete blocking results, a saturated amount of purified antibody ( le 5.0 μg/million cells) should be used for incubation with target cells, prior to staining with fluorochrome conjugated antibody. Immunohistochemistry-frozen/paraffin. It is recommended that the reagent be titrated for optimal performance for each application.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN263069) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS ( pH 7.2)
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN263069)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Product cited in:

Baker, Murphy, Lopez, Garcia: "Conversion of a capture ELISA to a Luminex xMAP assay using a multiplex antibody screening method." in: Journal of visualized experiments : JoVE, Issue 65, 2012 (Sample species: Human). Further details: ELISA

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN263069) back to top
Supplier Images
ELISA image for anti-Tumor Necrosis Factor antibody (TNF)  (Biotin) (ABIN263069) anti-Tumor Necrosis Factor (TNF) antibody (Biotin)