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anti-Human Tumor Necrosis Factor antibody for Immunocytochemistry

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Reactivity
Baboon, Human, Pig (Porcine), Primate
1215
443
357
142
135
119
119
55
52
41
28
17
11
8
8
7
6
4
4
4
4
2
1
1
1
Host
Mouse
923
482
182
42
10
9
7
6
3
2
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is conjugated to Biotin
159
95
70
49
32
30
27
26
25
25
25
25
25
25
24
24
24
19
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), ELISA
864
839
591
307
273
229
200
179
137
119
97
63
27
26
25
22
20
16
14
8
7
6
6
6
5
5
5
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
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Catalog No. ABIN263069
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN263070 ICC FACS IF IHC (p) FITC Mouse IgG1 kappa Log in to see MAb11 1
1 ABIN263068 ELISA FACS ICC IF IHC IHC (fro) IHC (p) Mouse IgG1 kappa Log in to see MAb11 1
1 ABIN446837 ICC IF IHC IHC (ff) IHC (fro) IHC (p) WB Rabbit AA 100-200, Internal Region Log in to see Polyclonal 11
1 ABIN2506723 ICC IF IHC (p) Rabbit IgG Log in to see Polyclonal
1 ABIN957936 ICC WB Mouse IgG1 Log in to see 28401
1 ABIN1981885 Func IHC (f) ICC FACS ELISA FITC Mouse IgG1 Log in to see MAb11 5
1 ABIN1690740 ELISA FACS Func ICC IHC (f) PE Mouse IgG1 Log in to see MAb11 5
1 ABIN1172395 ICC IHC IP WB Rabbit IgG AA 77-233 Log in to see Polyclonal
1 ABIN3201314 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 77-233 Log in to see
1 ABIN2689935 ICC Mouse IgG1, kappa Log in to see MAb11 2
1 ABIN4360942 FACS ICC IF IHC (fro) IHC (p) Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361084 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 680 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361087 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 755 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4360941 FACS ICC IF IHC (fro) IHC (p) Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361082 ELISA ICC IF IHC (fro) IHC (p) Biotin Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361078 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361080 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361085 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 488 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361090 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 550 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361092 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 405 Mouse IgM kappa N-Term Log in to see TNF706

Top referenced anti-Tumor Necrosis Factor antibody for Immunocytochemistry

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Primate Pig (Porcine)
Blocking Reagent (BR) 1 Antibodies 1 Antibodies
ELISA 7 Antibodies 40 Antibodies
Flow Cytometry (FACS) 5 Antibodies 18 Antibodies
Functional Studies (Func) 1 Antibodies 17 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 18 Antibodies
Immunofluorescence (IF) 4 Antibodies 5 Antibodies
Immunohistochemistry (IHC) 4 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 6 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Neutralization (Neut) 1 Antibodies
Radioimmunoassay (RIA) 1 Antibodies
Western Blotting (WB) 4 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Baboon, Human, Pig (Porcine), Primate
(1215), (443), (357), (142), (135), (119), (119), (55), (52), (41), (28), (17), (11), (8), (8), (7), (6), (4), (4), (4), (4), (2), (1), (1), (1)
Host Mouse
(923), (482), (182), (42), (10), (9), (7), (6), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is conjugated to Biotin
(159), (95), (70), (49), (32), (30), (27), (26), (25), (25), (25), (25), (25), (25), (24), (24), (24), (19), (13), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), ELISA
(864), (839), (591), (307), (273), (229), (200), (179), (137), (119), (97), (63), (27), (26), (25), (22), (20), (16), (14), (8), (7), (6), (6), (6), (5), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN263069) Images
Specificity TNF- alpha (MAb11)
Purification Protein A or G purified
Immunogen E. coli-expressed, recombinant human TNF- alpha
Clone MAb11
Isotype IgG1 kappa

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN263069) Images back to top
Antigen
Alternative Name TNF-alpha (TNF Antibody Abstract)
Background Gene Symbol: TNF
Gene ID 7124
Research Area Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN263069) Images back to top
Application Notes Flow Cytometry 1:10-1:1000, ELISA 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:10-1:2000, Sandwich ELISAThe purified MAb11 has been tested by blocking fluorochrome conjugated MAb11 for intracellular cytokine staining. In order to obtain complete blocking results, a saturated amount of purified antibody ( le 5.0 μg/million cells) should be used for incubation with target cells, prior to staining with fluorochrome conjugated antibody. Immunohistochemistry-frozen/paraffin. It is recommended that the reagent be titrated for optimal performance for each application.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN263069) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS ( pH 7.2)
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

References for anti-Tumor Necrosis Factor Antibody (ABIN263069)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Product cited in:

Baker, Murphy, Lopez, Garcia: "Conversion of a capture ELISA to a Luminex xMAP assay using a multiplex antibody screening method." in: Journal of visualized experiments : JoVE, Issue 65, 2012 (Sample species: Human). Further details: ELISA

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN263069) back to top
Supplier Images
ELISA image for anti-Tumor Necrosis Factor antibody (TNF)  (Biotin) (ABIN263069) anti-Tumor Necrosis Factor (TNF) antibody (Biotin)