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anti-Human Tumor Necrosis Factor antibody for Immunocytochemistry

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Reactivity
Baboon, Human, Pig (Porcine), Primate
1228
446
339
139
132
118
118
55
54
31
26
18
8
7
7
6
4
4
4
4
2
2
1
1
1
Host
Mouse
929
490
201
44
13
9
7
6
3
2
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is conjugated to Biotin
167
98
69
49
32
30
27
26
25
25
25
25
25
25
24
24
24
18
13
9
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), ELISA
885
808
595
299
266
230
206
185
141
128
101
63
27
26
25
22
18
14
8
8
6
6
5
5
5
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN263069
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN263068 ELISA FACS ICC IF IHC IHC (fro) IHC (p) Mouse IgG1 kappa Log in to see MAb11 1
1 ABIN263070 ICC FACS IF IHC (p) FITC Mouse IgG1 kappa Log in to see MAb11 1
1 ABIN446837 ICC IF IHC IHC (ff) IHC (fro) IHC (p) WB Rabbit AA 100-200, Internal Region Log in to see Polyclonal 11
1 ABIN2506723 ICC IF IHC (p) Rabbit IgG Log in to see Polyclonal
1 ABIN957936 ICC WB Mouse IgG1 Log in to see 28401
1 ABIN1981885 Func IHC (f) ICC FACS ELISA FITC Mouse IgG1 Log in to see MAb11 5
1 ABIN1690740 ELISA FACS Func ICC IHC (f) PE Mouse IgG1 Log in to see MAb11 5
1 ABIN1172395 ICC IHC IP WB Rabbit IgG AA 77-233 Log in to see Polyclonal
1 ABIN3201314 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 77-233 Log in to see
1 ABIN4361084 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 680 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361077 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361083 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 650 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361079 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 647 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361080 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361085 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 488 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361090 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 550 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361092 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 405 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361082 ELISA ICC IF IHC (fro) IHC (p) Biotin Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361078 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4361087 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 755 Mouse IgM kappa N-Term Log in to see TNF706

Top referenced anti-Tumor Necrosis Factor antibody for Immunocytochemistry

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Primate Pig (Porcine)
ELISA 6 Antibodies 41 Antibodies
Flow Cytometry (FACS) 3 Antibodies 15 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 21 Antibodies
Immunofluorescence (IF) 3 Antibodies 9 Antibodies
Immunohistochemistry (IHC) 4 Antibodies 16 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies 4 Antibodies
Western Blotting (WB) 4 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Functional Studies (Func) 20 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 4 Antibodies
Immunoprecipitation (IP) 2 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Baboon, Human, Pig (Porcine), Primate
(1228), (446), (339), (139), (132), (118), (118), (55), (54), (31), (26), (18), (8), (7), (7), (6), (4), (4), (4), (4), (2), (2), (1), (1), (1)
Host Mouse
(929), (490), (201), (44), (13), (9), (7), (6), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is conjugated to Biotin
(167), (98), (69), (49), (32), (30), (27), (26), (25), (25), (25), (25), (25), (25), (24), (24), (24), (18), (13), (9), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), ELISA
(885), (808), (595), (299), (266), (230), (206), (185), (141), (128), (101), (63), (27), (26), (25), (22), (18), (14), (8), (8), (6), (6), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN263069) Images
Specificity TNF- alpha (MAb11)
Cross-Reactivity (Details) Predicted cross-reactivity based on sequence identity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Sooty Mangabey, Swine (Pig, Porcine).
Purification Protein A or G purified
Immunogen E. coli-expressed, recombinant human TNF- alpha
Clone MAb11
Isotype IgG1 kappa

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN263069) Images back to top
Antigen
Alternative Name TNF-alpha (TNF Antibody Abstract)
Background Gene Symbol: TNF
Gene ID 7124
Research Area Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN263069) Images back to top
Application Notes Flow Cytometry 1:10-1:1000, ELISA 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:10-1:2000, Sandwich ELISAThe purified MAb11 has been tested by blocking fluorochrome conjugated MAb11 for intracellular cytokine staining. In order to obtain complete blocking results, a saturated amount of purified antibody ( le 5.0 μg/million cells) should be used for incubation with target cells, prior to staining with fluorochrome conjugated antibody. Immunohistochemistry-frozen/paraffin. It is recommended that the reagent be titrated for optimal performance for each application.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN263069) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS ( pH 7.2)
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

References for anti-Tumor Necrosis Factor Antibody (ABIN263069)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Product cited in:

Baker, Murphy, Lopez, Garcia: "Conversion of a capture ELISA to a Luminex xMAP assay using a multiplex antibody screening method." in: Journal of visualized experiments : JoVE, Issue 65, 2012 (Sample species: Human). Further details: ELISA

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN263069) back to top
Supplier Images
ELISA image for anti-Tumor Necrosis Factor antibody (TNF)  (Biotin) (ABIN263069) anti-Tumor Necrosis Factor (TNF) antibody (Biotin)