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anti-Human Tumor Necrosis Factor antibody for Immunohistochemistry

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Reactivity
Human
704
308
142
55
52
31
31
27
23
18
12
12
8
7
5
4
4
4
3
3
2
2
1
1
1
Host
Rabbit
538
393
157
43
13
9
9
7
4
1
Clonality
Polyclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
147
83
44
24
20
9
9
7
5
4
4
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Neutralization (Neut), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
632
586
319
207
119
100
99
86
80
63
56
56
25
25
22
22
15
14
8
8
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN1043796
$ 135.00
Plus shipping costs $45.00 and $21.00 dry ice
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.22030146 ABIN1582064 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal 2
0.22030146 ABIN1533583 IF IHC ELISA Rabbit IgG Log in to see Polyclonal
0.22030146 ABIN2707185 IHC WB Rabbit Center Log in to see Polyclonal
0.22030146 ABIN2470862 IHC ELISA Mouse IgG1 kappa Log in to see MAb1
0.22030146 ABIN3020863 IHC WB Rabbit IgG C-Term Log in to see Polyclonal
0.22030146 ABIN107946 IHC Neut ELISA WB Rabbit IgG Log in to see Polyclonal
0.22030146 ABIN2500447 IF IHC ELISA Rabbit IgG Log in to see Polyclonal
0.22030146 ABIN2471395 IHC ELISA WB Mouse IgG1 kappa AA 1-234 Log in to see M 1 C4
0.22030146 ABIN2428843 IHC ELISA Rabbit IgG Log in to see Polyclonal
0.22030146 ABIN1846088 ICC IF IHC Rabbit Log in to see Polyclonal
0.22030146 ABIN1849100 ICC IF IHC ELISA Rabbit Internal Region Log in to see Polyclonal
0.0011371465 ABIN1582141 IHC ELISA WB Rabbit Middle Region Log in to see Polyclonal 5
0.0011371465 ABIN610451 Func FACS IHC WB FITC Mouse IgG1 Log in to see J1D9 2
0.0011371465 ABIN610452 Func FACS IHC WB PE Mouse IgG1 Log in to see J1D9 2
0.0011371465 ABIN2659787 BR FACS IF IHC PE/Dazzle™ 594 Mouse IgG1 kappa Log in to see MAb11 10
0.0011371465 ABIN1582139 IP IHC ELISA WB Mouse IgG Log in to see 2
0.0011371465 ABIN2665897 FACS IHC WB Mouse IgG1 kappa Log in to see MAb1 6
0.0011371465 ABIN284821 Inhibition IHC ELISA Mouse IgG1 Log in to see 1D9
0.0011371465 ABIN1875123 IHC WB Rabbit IgG C-Term Log in to see Polyclonal
0.0011371465 ABIN222906 IHC Neut WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-Tumor Necrosis Factor antibody for Immunohistochemistry

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 13 Antibodies
Blocking Reagent (BR) 2 Antibodies
Cellular Assay (CA) 2 Antibodies
Dot Blot (DB) 4 Antibodies
ELISA 341 Antibodies
ELISA (Capture) 6 Antibodies
ELISA (Detection) 4 Antibodies
ELISpot 9 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 3 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 2 Antibodies
Enzyme Immunoassay (EIA) 42 Antibodies
Flow Cytometry (FACS) 217 Antibodies
Functional Studies (Func) 68 Antibodies
Immunoassay (IA) 13 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 31 Antibodies
Immunofluorescence (IF) 50 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Human
(704), (308), (142), (55), (52), (31), (31), (27), (23), (18), (12), (12), (8), (7), (5), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1)
Host Rabbit
(538), (393), (157), (43), (13), (9), (9), (7), (4), (1)
Clonality
Polyclonal
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(147), (83), (44), (24), (20), (9), (9), (7), (5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Neutralization (Neut), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(632), (586), (319), (207), (119), (100), (99), (86), (80), (63), (56), (56), (25), (25), (22), (22), (15), (14), (8), (8), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN1043796) Images
Specificity This antibody is primarily directed against mature 17,000 MW human TNFalpha and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFalpha in the same formats using similar dilutions. The antibody does not recognize human TNGß (lymphotoxin). This IgG fraction antibody will recognize the cell-bound precursor of TNFalpha as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFalpha activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
Characteristics Anti TNF alpha Antibody recognizes TNF alpha (TNF, cachexin, cachectin, tumor necrosis factor-alpha or TNF-alpha) a cytokine involved in systemic inflammation. TNF alpha is a member of a group of cytokines that stimulate the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by other cell types as well. The primary role of TNF alpha is in the regulation of immune cells. TNFis an endogenous pyrogen that is able to induce fever, apoptotic cell death, sepsis (through IL-1 & IL-6 production), cachexia, inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNF production has been implicated in a variety of human diseases, including Alzheimer's disease, cancer, major depression, and inflammatory bowel disease (IBD).
Sterility Sterile filtered
Immunogen The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human TNFalpha produced in E.coli.
Immunogen Type: RecombinantProtein
Isotype IgG

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN1043796) Images back to top
Antigen
Alternative Name TNF alpha (TNF Antibody Abstract)
Background Anti TNF alpha Antibody recognizes TNF alpha (TNF, cachexin, cachectin, tumor necrosis factor-alpha or TNF-α) a cytokine involved in systemic inflammation. TNF alpha is a member of a group of cytokines that stimulate the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by other cell types as well. The primary role of TNF alpha is in the regulation of immune cells. TNFis an endogenous pyrogen that is able to induce fever, apoptotic cell death, sepsis (through IL-1 & IL-6 production), cachexia, inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNF production has been implicated in a variety of human diseases, including Alzheimer's disease, cancer, major depression, and inflammatory bowel disease (IBD).
Synonyms: APC1 antibody, Cachectin antibody, DIF antibody, Differentiation inducing factor antibody, Macrophage cytotoxic factor antibody, MCF antibody, Necrosin antibody
Gene ID 7124
UniProt P01375
Research Area Cardiovascular, Atherosclerosis, Growth Factors, Innate Immunity, Cytokines, Receptors, Immunology, Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN1043796) Images back to top
Application Notes This IgG fraction antibody of anti-Human TNFα has been tested for use in neutralizations, ELISA, immunohistochemistry and immunoblotting. It recognizes the 17,000 MW TNFα. Reactivity in other immunoassays is unknown.
Comment

Gene Name: TNFA

Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN1043796) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Without preservative
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Expiry Date 12 months

References for anti-Tumor Necrosis Factor Antibody (ABIN1043796)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Product cited in:

Kant, Swat, Zhang, Zhang, Neel, Flavell, Davis: "TNF-stimulated MAP kinase activation mediated by a Rho family GTPase signaling pathway." in: Genes & development, Vol. 25, Issue 19, pp. 2069-78, 2011

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN1043796) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor antibody (TNF) (ABIN1043796) Immunohistochemistry using polyclonal TNFa antibody showing staining of formalin/PFA-...
 image for anti-Tumor Necrosis Factor antibody (TNF) (ABIN1043796) anti-Tumor Necrosis Factor (TNF) antibody (Image 2)
 image for anti-Tumor Necrosis Factor antibody (TNF) (ABIN1043796) anti-Tumor Necrosis Factor (TNF) antibody (Image 3)