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anti-Human Tumor Necrosis Factor antibody for Immunostaining

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Reactivity
Human
894
329
142
56
52
31
31
27
24
18
12
12
8
7
5
4
4
4
3
3
2
2
1
1
1
1
Host
Rabbit
672
432
196
43
13
9
9
7
4
1
Clonality
Polyclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
158
92
55
36
20
20
15
15
12
11
11
11
11
11
11
11
11
11
11
9
7
5
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunoassay (IA), Flow Cytometry (FACS), Immunostaining (ISt), Neutralization (Neut), Western Blotting (WB)
753
704
392
208
137
136
99
98
87
63
56
56
25
25
22
22
18
15
14
8
8
6
6
5
5
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN2192175
$ 532.13
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008877907 ABIN641298 CA DB IA ISt ELISA WB Biotin Mouse IgG1 Log in to see
0.0008877907 ABIN641297 CA DB IA ISt ELISA WB Mouse IgG1 Log in to see

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 13 Antibodies
Blocking Reagent (BR) 2 Antibodies
Cellular Assay (CA) 2 Antibodies
Dot Blot (DB) 4 Antibodies
ELISA 459 Antibodies
ELISA (Capture) 6 Antibodies
ELISA (Detection) 4 Antibodies
ELISpot 9 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 3 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 2 Antibodies
Enzyme Immunoassay (EIA) 42 Antibodies
Flow Cytometry (FACS) 289 Antibodies
Functional Studies (Func) 68 Antibodies
Immunoassay (IA) 14 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 31 Antibodies
Immunofluorescence (IF) 50 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Human
(894), (329), (142), (56), (52), (31), (31), (27), (24), (18), (12), (12), (8), (7), (5), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(672), (432), (196), (43), (13), (9), (9), (7), (4), (1)
Clonality
Polyclonal
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(158), (92), (55), (36), (20), (20), (15), (15), (12), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (9), (7), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunoassay (IA), Flow Cytometry (FACS), Immunostaining (ISt), Neutralization (Neut), Western Blotting (WB)
(753), (704), (392), (208), (137), (136), (99), (98), (87), (63), (56), (56), (25), (25), (22), (22), (18), (15), (14), (8), (8), (6), (6), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling Images
Sterility 0.2 μm filtered

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling Images back to top
Antigen
Alternative Name Tumor Necrosis Factor-alpha (TNF Antibody Abstract)
Background The antibody reacts with human natural and recombinant TNF-alpha as assessed by ELISA. The antibody inhibits the biological activity of human natural and recombinant TNF-alpha as determined with L929 and WEHI cells in a cytotoxicity assay. The antibody cross reacts with rhesus and cynomolgus natural TNF- alpha and lacks cross reactivity with human lymphotoxin.
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling Images back to top
Application Notes For flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For neutralization of biological activity dilutions have to be made according to the amounts TNF-alpha to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Images back to top
Buffer PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Expiry Date 12 months