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anti-Human Tumor Necrosis Factor antibody for Neutralization

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Reactivity
Human
1199
442
329
139
132
118
118
55
55
31
26
18
8
8
7
7
4
4
4
3
2
2
2
1
1
1
Host
Rabbit
915
476
201
44
13
9
7
4
3
1
Clonality
Polyclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
167
95
69
49
32
30
27
26
25
25
25
25
25
25
24
24
24
18
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Neutralization (Neut), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
852
796
536
253
250
163
141
139
134
124
101
63
25
24
23
22
18
14
8
6
6
5
5
5
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN1043796
$ 135.00
Plus shipping costs $45.00 and $21.00 dry ice
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008885278 ABIN1176946 Neut ELISA Mouse IgG1 Log in to see MAb1 2
0.0008885278 ABIN1177286 Neut ELISA Mouse IgG2a, kappa Log in to see MABTNF-A5 3
0.0008885278 ABIN222906 IHC Neut WB Rabbit IgG Log in to see Polyclonal
0.0008885278 ABIN465605 Neut ELISA Mouse IgG1 Log in to see
0.0008885278 ABIN1449154 Neut Mouse IgG1 Log in to see #1
0.0008885278 ABIN107946 IHC Neut ELISA WB Rabbit IgG Log in to see Polyclonal
0.0008885278 ABIN465030 Neut ELISA WB Rabbit Log in to see Polyclonal
0.0008885278 ABIN465674 IHC (p) Neut ELISA WB Goat Log in to see Polyclonal
0.0008885278 ABIN1104720 IHC (p) Neut WB Rabbit Log in to see Polyclonal
0.0008885278 ABIN1983231 Neut IHC WB Mouse IgG1 AA 57-233 Log in to see MM0327-7J20
0.0008885278 ABIN603912 Neut ELISA Mouse IgG2a Log in to see
0.0008885278 ABIN199738 FACS Neut ELISA WB Rabbit IgG Log in to see Polyclonal
0.0008885278 ABIN1983458 Neut IHC WB Mouse IgG1 AA 57-233 Log in to see MM0574-3F11
0.0008885278 ABIN2380265 IC Neut ELISA WB Mouse IgG1 AA 57-233 Log in to see 3H364
0.0008885278 ABIN4360964 BP ELISA FACS ICC IF IHC (fro) IP Neut WB Mouse IgM kappa Log in to see MAb1
0.0008885278 ABIN4360963 BP ELISA FACS ICC IF IHC (fro) IP Neut WB Mouse IgM kappa Log in to see MAb1
0.0008885278 ABIN199734 ICC Neut ELISA WB Mouse IgG1 Log in to see
0.0008885278 ABIN2377415 Neut IHC WB Rabbit IgG Log in to see Polyclonal
0.0008885278 ABIN2380270 FACS Neut ELISA WB Rabbit IgG Log in to see Polyclonal
0.0008885278 ABIN2380272 FACS IP Neut WB Biotin Mouse IgG1 Log in to see 6A651

Top referenced anti-Tumor Necrosis Factor antibody for Neutralization

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 13 Antibodies
Blocking Peptide (BP) 20 Antibodies
Blocking Reagent (BR) 2 Antibodies
Cellular Assay (CA) 2 Antibodies
Dot Blot (DB) 4 Antibodies
ELISA 498 Antibodies
ELISA (Capture) 4 Antibodies
ELISA (Detection) 3 Antibodies
ELISpot 9 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Enzyme Immunoassay (EIA) 42 Antibodies
Flow Cytometry (FACS) 437 Antibodies
Functional Studies (Func) 71 Antibodies
Immunoassay (IA) 15 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 95 Antibodies
Immunofluorescence (IF) 132 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Human
(1199), (442), (329), (139), (132), (118), (118), (55), (55), (31), (26), (18), (8), (8), (7), (7), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1)
Host Rabbit
(915), (476), (201), (44), (13), (9), (7), (4), (3), (1)
Clonality
Polyclonal
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(167), (95), (69), (49), (32), (30), (27), (26), (25), (25), (25), (25), (25), (25), (24), (24), (24), (18), (13), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Neutralization (Neut), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(852), (796), (536), (253), (250), (163), (141), (139), (134), (124), (101), (63), (25), (24), (23), (22), (18), (14), (8), (6), (6), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN1043796) Images
Specificity This antibody is primarily directed against mature 17,000 MW human TNFalpha and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFalpha in the same formats using similar dilutions. The antibody does not recognize human TNGß (lymphotoxin). This IgG fraction antibody will recognize the cell-bound precursor of TNFalpha as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFalpha activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
Characteristics Anti TNF alpha Antibody recognizes TNF alpha (TNF, cachexin, cachectin, tumor necrosis factor-alpha or TNF-alpha) a cytokine involved in systemic inflammation. TNF alpha is a member of a group of cytokines that stimulate the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by other cell types as well. The primary role of TNF alpha is in the regulation of immune cells. TNFis an endogenous pyrogen that is able to induce fever, apoptotic cell death, sepsis (through IL-1 & IL-6 production), cachexia, inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNF production has been implicated in a variety of human diseases, including Alzheimer's disease, cancer, major depression, and inflammatory bowel disease (IBD).
Sterility Sterile filtered
Immunogen The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human TNFalpha produced in E.coli.
Immunogen Type: RecombinantProtein
Isotype IgG

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN1043796) Images back to top
Antigen
Alternative Name TNF alpha (TNF Antibody Abstract)
Background Anti TNF alpha Antibody recognizes TNF alpha (TNF, cachexin, cachectin, tumor necrosis factor-alpha or TNF-α) a cytokine involved in systemic inflammation. TNF alpha is a member of a group of cytokines that stimulate the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by other cell types as well. The primary role of TNF alpha is in the regulation of immune cells. TNFis an endogenous pyrogen that is able to induce fever, apoptotic cell death, sepsis (through IL-1 & IL-6 production), cachexia, inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNF production has been implicated in a variety of human diseases, including Alzheimer's disease, cancer, major depression, and inflammatory bowel disease (IBD).
Synonyms: APC1 antibody, Cachectin antibody, DIF antibody, Differentiation inducing factor antibody, Macrophage cytotoxic factor antibody, MCF antibody, Necrosin antibody
Gene ID 7124
UniProt P01375
Research Area Cardiovascular, Atherosclerosis, Growth Factors, Innate Immunity, Cytokines, Receptors, Immunology, Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN1043796) Images back to top
Application Notes This IgG fraction antibody of anti-Human TNFα has been tested for use in neutralizations, ELISA, immunohistochemistry and immunoblotting. It recognizes the 17,000 MW TNFα. Reactivity in other immunoassays is unknown.
Comment

Gene Name: TNFA

Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN1043796) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Without preservative
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Expiry Date 12 months

References for anti-Tumor Necrosis Factor Antibody (ABIN1043796)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Product cited in:

Kant, Swat, Zhang, Zhang, Neel, Flavell, Davis: "TNF-stimulated MAP kinase activation mediated by a Rho family GTPase signaling pathway." in: Genes & development, Vol. 25, Issue 19, pp. 2069-78, 2011

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN1043796) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor antibody (TNF) (ABIN1043796) Immunohistochemistry using polyclonal TNFa antibody showing staining of formalin/PFA-...
 image for anti-Tumor Necrosis Factor antibody (TNF) (ABIN1043796) anti-Tumor Necrosis Factor (TNF) antibody (Image 2)
 image for anti-Tumor Necrosis Factor antibody (TNF) (ABIN1043796) anti-Tumor Necrosis Factor (TNF) antibody (Image 3)