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|Application / Reactivity||Monkey||Human|
|ELISA||14 Antibodies||498 Antibodies|
|Enzyme Immunoassay (EIA)||8 Antibodies|
|Flow Cytometry (FACS)||10 Antibodies|
|Functional Studies (Func)||14 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||8 Antibodies|
|Immunofluorescence (IF)||5 Antibodies|
|Immunohistochemistry (IHC)||6 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||8 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||2 Antibodies|
|Immunoprecipitation (IP)||5 Antibodies|
|Western Blotting (WB)||12 Antibodies|
|Biochemical Assay (BCA)||1 Antibodies|
|Blocking Antibody (Inhibition)||13 Antibodies|
|Blocking Peptide (BP)||20 Antibodies|
|Blocking Reagent (BR)||2 Antibodies|
|Cellular Assay (CA)||2 Antibodies|
|Dot Blot (DB)||4 Antibodies|
|Antigen||Tumor Necrosis Factor (TNF) Antibodies|
|Reactivity||Human, Monkey Alternatives|
|Conjugate||This Tumor Necrosis Factor antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-Tumor Necrosis Factor AntibodyTarget Details Tumor Necrosis Factor Application Details Handling Images
|Characteristics||Synonyms: TNF, TNF-a, TNFA, TNFSF2, Cachectin, Tumor necrosis factor ligand superfamily member 2|
|Purification||Protein G Chromatography|
|Purity||> 95 % by SDS-PAGE|
Target Details Tumor Necrosis FactorProduct Details anti-Tumor Necrosis Factor Antibody Application Details Handling Images back to top
|Alternative Name||Tumor Necrosis Factor (TNF-Alpha) (TNF Antibody Abstract)|
|Background||Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2|
|Research Area||Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Cancer, Virology|
|Pathways||NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling Images back to top
Western Blot: This antibody has been used at a range from 1 to 5 μg/mL to visualize human TNFa. Immunohistochemistry: This antibody has been used at 1-10 μg/mL to stain human TNFaexpressed in mononuclear cells, following fixation with 4 % paraform in PBS for 10 minutesat 4C, plus 0.1 % saponin 20 minutes at RT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Images back to top
|Buffer||PBS containing 0.08 % Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
|Expiry Date||12 months|