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anti-Mouse (Murine) Tumor Necrosis Factor antibody for ELISpot

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Reactivity
Mouse (Murine)
705
307
142
55
52
31
31
27
23
18
12
12
8
7
5
4
4
4
3
3
2
2
1
1
1
Host
Rat
538
394
156
43
13
9
9
7
4
1
Clonality
Monoclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
147
83
44
24
20
9
9
7
5
4
4
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
ELISA (Capture), ELISpot
633
587
319
208
120
100
99
86
80
63
56
56
25
25
22
22
14
14
8
7
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN281689
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008755788 ABIN475640 ELISpot ELISA Biotin Rabbit IgG Log in to see Polyclonal
0.0008755788 ABIN2749007 ELISpot ELISA Biotin Rabbit IgG Log in to see Polyclonal
0.0008755788 ABIN1177367 ELISpot Log in to see

Top referenced anti-Tumor Necrosis Factor antibody for ELISpot

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 2 Antibodies
Blocking Antibody (Inhibition) 7 Antibodies
Blocking Reagent (BR) 3 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 142 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
ELISpot 4 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Enzyme Immunoassay (EIA) 11 Antibodies
FLISA 1 Antibodies
Flow Cytometry (FACS) 107 Antibodies
Functional Studies (Func) 19 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 9 Antibodies
Immunofluorescence (IF) 25 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 48 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 2 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Mouse (Murine)
(705), (307), (142), (55), (52), (31), (31), (27), (23), (18), (12), (12), (8), (7), (5), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1)
Host Rat
(538), (394), (156), (43), (13), (9), (9), (7), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(147), (83), (44), (24), (20), (9), (9), (7), (5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application ELISA (Capture), ELISpot
(633), (587), (319), (208), (120), (100), (99), (86), (80), (63), (56), (56), (25), (25), (22), (22), (14), (14), (8), (7), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN281689) Images
Specificity The 1F3F3D4 antibody reacts with mouse tumor necrosis factor-alpha (TNFaalpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4(+) T cells.
Characteristics The 1F3F3D4 antibody reacts with mouse tumor necrosis factor-alpha (TNFaalpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4(+) T cells.
Synonyms: Synonyms: Tumor Necrosis Factor alpha, TNFa TNF-a
Purification Affinity Purified
Clone 1F3F3D4
Isotype IgM kappa

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN281689) Images back to top
Antigen
Alternative Name TNFa (TNF Antibody Abstract)
Research Area Cardiovascular, Atherosclerosis, Growth Factors, Innate Immunity, Cytokines, Receptors, Immunology, Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN281689) Images back to top
Application Notes Applications reported: The 1F3F3D4 antibody has been reported for use in capture of mouse TNF alpha by ELISA and ELISPOT.

Applications tested: The 1F3F3D4 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse TNF alpha (Tumor Necrosis Factor alpha). A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL.
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN281689) Images back to top
Concentration 0.5 mg/mL
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
Storage 4 °C

References for anti-Tumor Necrosis Factor Antibody (ABIN281689)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Background publications

Lucas, Heirwegh, Neirynck, Remels, Van Heuverswyn, De Baetselier: "Generation and characterization of a neutralizing rat anti-rmTNF-alpha monoclonal antibody." in: Immunology, Vol. 71, Issue 2, pp. 218-23, 1990

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN281689) back to top
Supplier Images
 image for anti-Tumor Necrosis Factor antibody (TNF) (ABIN281689) Mouse splenocytes (500,000 cells per well) were activated with anti -CD3 for 48 hrs i...
 image for anti-Tumor Necrosis Factor antibody (TNF) (ABIN281689) anti-Tumor Necrosis Factor (TNF) antibody (Image 2)