anti-Mouse (Murine) Tumor Necrosis Factor antibody for ELISpot

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Reactivity
Mouse (Murine)
1251
460
359
145
136
119
119
63
55
41
29
18
14
12
9
7
6
4
4
4
4
2
1
1
1
1
Host
Rabbit
935
518
184
48
10
9
7
6
4
3
2
Clonality
Polyclonal
Conjugate
This Tumor Necrosis Factor antibody is conjugated to Biotin
168
100
74
50
32
30
28
27
26
25
25
25
25
25
24
24
24
23
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
ELISpot, ELISA
886
858
622
321
287
230
219
190
138
118
97
69
29
29
26
22
20
18
13
9
7
7
6
6
6
5
5
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
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Catalog No. ABIN475640
$ 361.17
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2749007 ELISpot ELISA Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN1177367 ELISpot Log in to see

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Mouse (Murine)
(1251), (460), (359), (145), (136), (119), (119), (63), (55), (41), (29), (18), (14), (12), (9), (7), (6), (4), (4), (4), (4), (2), (1), (1), (1), (1)
Host Rabbit
(935), (518), (184), (48), (10), (9), (7), (6), (4), (3), (2)
Clonality
Polyclonal
Conjugate This Tumor Necrosis Factor antibody is conjugated to Biotin
(168), (100), (74), (50), (32), (30), (28), (27), (26), (25), (25), (25), (25), (25), (24), (24), (24), (23), (13), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application ELISpot, ELISA
(886), (858), (622), (321), (287), (230), (219), (190), (138), (118), (97), (69), (29), (29), (26), (22), (20), (18), (13), (9), (7), (7), (6), (6), (6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling Images
Purification Affinity purified
Immunogen Mouse TNF
Isotype IgG

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling Images back to top
Antigen
Alternative Name TNF Alpha (TNF Antibody Abstract)
Background Name/Gene ID: TNF
Family: TNF

Synonyms: TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2
Gene ID 7124
UniProt P01375
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling Images back to top
Application Notes Approved: ELISA, ELISPOT

Usage: The biotinylated rabbit anti-mouse/rat TNF-alpha polyclonal antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse or rat TNF-alpha. A suitable range of concentrations of this biotinylated antibody for ELISA detection is 0.5-2.0 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL (mouse TNF-alpha) or 5000 - 30 pg/mL (rat TNF-alpha) should be included in each ELISA plate. Recognition of mouse TNF-alpha by this assay is not interfered with by 1000x excess soluble TNF-&alph. receptor type I or II. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, pH 7.2, 150 mM sodium chloride, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.