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|Antigen||Tumor Necrosis Factor (TNF) Antibodies|
|Reactivity||Human, Non-Human Primate, Pig (Porcine) Alternatives|
|Conjugate||This Tumor Necrosis Factor antibody is conjugated to FITC Alternatives|
Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA
|5 references available|
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Product Details anti-Tumor Necrosis Factor AntibodyTarget Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN1981885) Images
|Specificity||The mouse monoclonal antibody MAb11 recognizes human 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha).|
|Characteristics||The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.|
|Immunogen||Recombinant human TNF-alpha|
Target Details Tumor Necrosis FactorProduct Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN1981885) Images back to top
|Alternative Name||TNF-alpha (TNF Antibody Abstract)|
|Background||TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.|
|Research Area||Inflammation, Cancer, Virology|
|Pathways||NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus|
Application DetailsProduct Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor antibody (ABIN1981885) Images back to top
|Application Notes||The reagent is designed for Flow Cytometry analysis of human blood cells using 4 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (0.4 ml) is sufficient for 100 tests.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor antibody (ABIN1981885) Images back to top
|Buffer||Phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.|
Do not freeze. Avoid prolonged exposure to light.
Do not use after expiration date stamped on vial label.
Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
|Storage Comment||Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.|
References for anti-Tumor Necrosis Factor antibody (ABIN1981885)Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
|Product cited in:||
Cesaro-Tadic, Dernick, Juncker, Buurman, Kropshofer, Michel, Fattinger, Delamarche: "High-sensitivity miniaturized immunoassays for tumor necrosis factor alpha using microfluidic systems." in: Lab on a chip, Vol. 4, Issue 6, pp. 563-9, 2004
Yan, Qing, Byers, Stadnyk, Al-Hertani, Bortolussi: "Role of MyD88 in diminished tumor necrosis factor alpha production by newborn mononuclear cells in response to lipopolysaccharide." in: Infection and immunity, Vol. 72, Issue 3, pp. 1223-9, 2004
Attarbaschi, Willheim, Ramharter, Hofmann, Wahl, Winkler, Graninger, Winkler: "T cell cytokine profile during primary Epstein-Barr virus infection (infectious mononucleosis)." in: European cytokine network, Vol. 14, Issue 1, pp. 34-9, 2003
Visser, Graffelman, Blauw, Haspels, Lentjes, de Kloet, Nagelkerken: "LPS-induced IL-10 production in whole blood cultures from chronic fatigue syndrome patients is increased but supersensitive to inhibition by dexamethasone." in: Journal of neuroimmunology, Vol. 119, Issue 2, pp. 343-9, 2001
Wahlström, Katchar, Wigzell, Olerup, Eklund, Grunewald: "Analysis of intracellular cytokines in CD4+ and CD8+ lung and blood T cells in sarcoidosis." in: American journal of respiratory and critical care medicine, Vol. 163, Issue 1, pp. 115-21, 2001