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anti-Rat (Rattus) Tumor Necrosis Factor antibody for Immunoprecipitation

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • Cachectin
  • cTNF
  • dif
  • DIF
  • RATTNF
  • TNF
  • tnf
  • TNF-a
  • TNF-ALPHA
  • Tnf-alpha
  • tnf-alpha
  • TNF-alpha
  • TNFa
  • TNFA
  • tnfa
  • Tnfa
  • tnfa-like
  • TNFalpha
  • Tnfsf1a
  • TNFSF2
  • tnfsf2
  • xtnf
Reactivity
Mouse (Murine), Rat (Rattus)
1242
464
345
144
138
123
123
56
56
45
31
18
8
8
7
7
4
4
4
3
3
2
2
1
1
1
Host
Goat
960
480
213
42
13
9
9
8
4
1
Clonality
Polyclonal
Conjugate
This Tumor Necrosis Factor antibody is conjugated to Biotin
172
99
71
49
35
31
29
27
27
26
26
26
26
26
26
25
25
25
20
9
7
5
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Mass Cytometry (CyTOF), Immunoprecipitation (IP), Immunohistochemistry (IHC)
883
805
581
258
254
167
146
144
135
124
104
63
25
25
24
22
22
19
15
8
8
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN2661299
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089556724 ABIN1582139 IP IHC ELISA WB Mouse IgG Log in to see 2
0.00089556724 ABIN2689936 Inhibition IP Neut ELISA WB Armenian Hamster IgG1, lambda Log in to see TN3 6
0.00089556724 ABIN265631 BP ELISA IP Neut WB Armenian Hamster IgG2a Log in to see TN3-19-12
0.00089556724 ABIN4360941 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa N-Term Log in to see TNF706
0.00089556724 ABIN4360943 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa N-Term Log in to see TNF706
0.00089556724 ABIN4360942 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa N-Term Log in to see TNF706
0.00089556724 ABIN4360987 FACS ICC IF IHC (fro) IHC (p) IP PE Mouse IgG1 kappa Log in to see SPM543
0.00089556724 ABIN4360988 FACS ICC IF IHC (fro) IHC (p) IP DyLight 550 Mouse IgG1 kappa Log in to see SPM543
0.00089556724 ABIN4360991 FACS ICC IF IHC (fro) IHC (p) IP DyLight 405LS Mouse IgG1 kappa Log in to see SPM543
0.00089556724 ABIN4360990 FACS ICC IF IHC (fro) IHC (p) IP DyLight 405 Mouse IgG1 kappa Log in to see SPM543
0.00089556724 ABIN4360985 FACS ICC IF IHC (fro) IHC (p) IP DyLight 755 Mouse IgG1 kappa Log in to see SPM543
0.00089556724 ABIN4360989 FACS ICC IF IHC (fro) IHC (p) IP DyLight 350 Mouse IgG1 kappa Log in to see SPM543
0.00089556724 ABIN4360986 FACS ICC IF IHC (fro) IHC (p) IP PerCP Mouse IgG1 kappa Log in to see SPM543
0.00089556724 ABIN4360935 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa Log in to see 4C6-H8
0.00089556724 ABIN4360936 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa Log in to see 4C6-H8
0.00089556724 ABIN4360937 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa Log in to see 4C6-H8
0.00089556724 ABIN4360938 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa Log in to see P-T2
0.00089556724 ABIN4360939 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa Log in to see P-T2
0.00089556724 ABIN4360940 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa Log in to see P-T2
0.00089556724 ABIN4360944 FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM Log in to see TNF706-P-T2

Top referenced anti-Tumor Necrosis Factor antibody for Immunoprecipitation

Similar anti-Tumor Necrosis Factor Antibodies

Application / Reactivity Rat (Rattus)
Blocking Antibody (Inhibition) 3 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 70 Antibodies
ELISA (Capture) 1 Antibodies
ELISA (Detection) 1 Antibodies
ELISpot 2 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 144 Antibodies
Functional Studies (Func) 8 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 67 Antibodies
Immunofluorescence (IF) 101 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 64 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 49 Antibodies

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Reactivity Mouse (Murine), Rat (Rattus)
(1242), (464), (345), (144), (138), (123), (123), (56), (56), (45), (31), (18), (8), (8), (7), (7), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1)
Host Goat
(960), (480), (213), (42), (13), (9), (9), (8), (4), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This Tumor Necrosis Factor antibody is conjugated to Biotin
(172), (99), (71), (49), (35), (31), (29), (27), (27), (26), (26), (26), (26), (26), (26), (25), (25), (25), (20), (9), (7), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Mass Cytometry (CyTOF), Immunoprecipitation (IP), Immunohistochemistry (IHC)
(883), (805), (581), (258), (254), (167), (146), (144), (135), (124), (104), (63), (25), (25), (24), (22), (22), (19), (15), (8), (8), (6), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN2661299) Images
Cross-Reactivity Rat (Rattus)
Purification The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Clone Poly5160
Isotype IgG

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN2661299) Images back to top
Antigen
Alternative Name TNF-alpha (TNF Antibody Abstract)
Background TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-α. Monomeric mouse TNF-α is 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD protein. TNF-α forms multimeric complexes, stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.
Research Area Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor Antibody (ABIN2661299) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor Antibody (ABIN2661299) Images back to top
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-Tumor Necrosis Factor Antibody (ABIN2661299)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Background publications

Tracey, Cerami: "Tumor necrosis factor: an updated review of its biology." in: Critical care medicine, Vol. 21, Issue 10 Suppl, pp. S415-22, 1993

Beutler, Cerami: "The biology of cachectin/TNF--a primary mediator of the host response." in: Annual review of immunology, Vol. 7, pp. 625-55, 1989

Beutler, Cerami: "Tumor necrosis, cachexia, shock, and inflammation: a common mediator." in: Annual review of biochemistry, Vol. 57, pp. 505-18, 1988

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor Antibody (ABIN2661299) back to top
Supplier Images
ELISA image for anti-Tumor Necrosis Factor antibody (TNF)  (Biotin) (ABIN2661299) anti-Tumor Necrosis Factor (TNF) antibody (Biotin)