anti-Rat (Rattus) Tumor Necrosis Factor antibody for Immunoprecipitation

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (TNF) Antibodies
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Epitope
AA 80-235
50
44
29
27
24
22
17
17
15
11
11
10
10
8
4
4
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Rat (Rattus)
1251
461
358
145
136
119
119
63
55
41
29
18
14
12
9
7
6
4
4
4
4
2
1
1
1
1
Host
Rabbit
935
518
184
48
10
9
7
6
4
3
2
Clonality
Polyclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
169
100
74
50
32
30
28
27
26
25
25
25
25
25
24
24
24
23
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
887
857
622
321
286
230
219
189
138
117
97
69
29
29
26
22
20
18
14
9
7
7
6
6
6
5
5
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Options
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Catalog No. ABIN1078627
$ 266.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1582139 IP IHC ELISA WB Mouse IgG Log in to see 2
1 ABIN2661299 CyTOF FACS IF IHC IP Biotin Goat IgG Log in to see Polyclonal 3
1 ABIN2689936 Inhibition IP Neut ELISA WB Armenian Hamster IgG1, lambda Log in to see TN3 6
1 ABIN4360975 ELISA FACS ICC IF IHC (fro) IHC (p) IP Alexa Fluor 405 Mouse IgG1 kappa Log in to see SPM543
1 ABIN4360976 ELISA FACS ICC IF IHC (fro) IHC (p) IP Alexa Fluor 488 Mouse IgG1 kappa Log in to see SPM543
1 ABIN4360977 ELISA FACS ICC IF IHC (fro) IHC (p) IP Alexa Fluor 647 Mouse IgG1 kappa Log in to see SPM543
1 ABIN4360978 ELISA FACS ICC IF IHC (fro) IHC (p) IP Alexa Fluor 700 Mouse IgG1 kappa Log in to see SPM543
1 ABIN4360981 ELISA FACS ICC IF IHC (fro) IHC (p) IP DyLight 650 Mouse IgG1 kappa Log in to see SPM543
1 ABIN4360982 ELISA FACS ICC IF IHC (fro) IHC (p) IP DyLight 680 Mouse IgG1 kappa Log in to see SPM543
1 ABIN4360983 ELISA FACS ICC IF IHC (fro) IHC (p) IP DyLight 488 Mouse IgG1 kappa Log in to see SPM543
1 ABIN4360985 ELISA FACS ICC IF IHC (fro) IHC (p) IP DyLight 755 Mouse IgG1 kappa Log in to see SPM543
1 ABIN4360986 FACS ICC IF IHC (fro) IHC (p) IP PerCP Mouse IgG1 kappa Log in to see SPM543
1 ABIN4360988 ELISA FACS ICC IF IHC (fro) IHC (p) IP DyLight 550 Mouse IgG1 kappa Log in to see SPM543
1 ABIN4360989 ELISA FACS ICC IF IHC (fro) IHC (p) IP DyLight 350 Mouse IgG1 kappa Log in to see SPM543
1 ABIN4360990 ELISA FACS ICC IF IHC (fro) IHC (p) IP DyLight 405 Mouse IgG1 kappa Log in to see SPM543
1 ABIN265631 BP ELISA IP Neut WB Armenian Hamster IgG2a Log in to see TN3-19-12
1 ABIN4360971 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN4360972 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP Mouse IgM kappa N-Term Log in to see TNF706
1 ABIN1492973 IHC (p) IP WB Magnetic Particles Rabbit C-Term Log in to see Polyclonal
1 ABIN4360959 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP Mouse IgG1 kappa Log in to see SPM543

General

Antigen Tumor Necrosis Factor (TNF) Antibodies
Epitope AA 80-235
(50), (44), (29), (27), (24), (22), (17), (17), (15), (11), (11), (10), (10), (8), (4), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Rat (Rattus)
(1251), (461), (358), (145), (136), (119), (119), (63), (55), (41), (29), (18), (14), (12), (9), (7), (6), (4), (4), (4), (4), (2), (1), (1), (1), (1)
Host Rabbit
(935), (518), (184), (48), (10), (9), (7), (6), (4), (3), (2)
Clonality
Polyclonal
Conjugate This Tumor Necrosis Factor antibody is un-conjugated
(169), (100), (74), (50), (32), (30), (28), (27), (26), (25), (25), (25), (25), (25), (24), (24), (24), (23), (13), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(887), (857), (622), (321), (286), (230), (219), (189), (138), (117), (97), (69), (29), (29), (26), (22), (20), (18), (14), (9), (7), (7), (6), (6), (6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-Tumor Necrosis Factor Antibody

Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN1078627) Images
Specificity The antibody is a rabbit polyclonal antibody raised against TNFa. It has been selected for its ability to recognize TNFa in immunohistochemical staining and western blotting.
Purification Antigen-specific affinity chromatography
Immunogen TNFa (Leu80-Leu235)
Isotype IgG

Target Details Tumor Necrosis Factor

Product Details anti-Tumor Necrosis Factor Antibody Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN1078627) Images back to top
Antigen
Alternative Name Tumor Necrosis Factor Alpha (TNFa) (TNF Antibody Abstract)
Background Alternative Names: DIF, TNF-A, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Handling References for anti-Tumor Necrosis Factor antibody (ABIN1078627) Images back to top
Application Notes
  • Western blotting: 0.5-2 μg/mL
  • Immunocytochemistry in formalin fixed cells: 5-20 μg/mL
  • Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL
  • Immunohistochemistry in paraffin section: 5-20 μg/mL
  • Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL
  • Optimal working dilutions must be determined by end user.
Comment

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions For Research Use only

Handling

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details References for anti-Tumor Necrosis Factor antibody (ABIN1078627) Images back to top
Format Liquid
Concentration 200 μg/mL
Buffer PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

References for anti-Tumor Necrosis Factor antibody (ABIN1078627)

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling Images back to top
Product cited in:

Chu, Zheng, Li, Zhang, Zhang, Xu, Chen: "Shuangtengbitong tincture treatment of collagen-induced arthritis via downregulation of the expression of IL-6, IL-8, TNF-? and NF-?B." in: Experimental and therapeutic medicine, Vol. 5, Issue 2, pp. 423-428, 2013

Wang, Zheng, Liang, Zhao, Qian, Liang, Feng: "Hepatocellular necrosis, apoptosis, and proliferation after transcatheter arterial embolization or chemoembolization in a standardized rabbit model." in: Journal of vascular and interventional radiology : JVIR, Vol. 22, Issue 11, pp. 1606-12, 2011

Volentine, Ogden, Kortje, Tuma, Sorrell: "Role of acetaldehyde in the ethanol-induced impairment of hepatic glycoprotein secretion in the rat in vivo." in: Hepatology (Baltimore, Md.), Vol. 7, Issue 3, pp. 490-5, 1987

Images

Product Details anti-Tumor Necrosis Factor Antibody Target Details Tumor Necrosis Factor Application Details Handling References for anti-Tumor Necrosis Factor antibody (ABIN1078627) back to top
Supplier Images
Western Blotting (WB) image for anti-Tumor Necrosis Factor (TNF) (AA 80-235) antibody (ABIN1078627) anti-Tumor Necrosis Factor (TNF) (AA 80-235) antibody