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anti-Human APAF1 antibody for Immunofluorescence

Recommended APAF1 Antibody (supplied by: Log in to see )

Antigen
Apoptotic Peptidase Activating Factor 1 (APAF1) Antibodies
  • 6230400I06Rik
  • anon-53Fa
  • apaf-1
  • Apaf-1
  • APAF-1
  • Apaf1
  • apaf1
  • APAF1
  • Apaf1l
  • arc
  • ARK
  • ark
  • CED4
  • CG6829
  • D-Apaf-1
  • dapaf
  • Dapaf-1
  • dAPAF-1
  • dapaf-1
  • dApaf-1
  • dApaf-1/DARK/HAC-1
  • Dapaf-1/HAC-1
  • dapaf-1L
  • dapaf-1S
  • dApaf1
  • DARK
  • dark
  • dArk
  • Dark
  • Dark/Apaf-I
  • dark/dapaf-1/hac-1
  • Dark/Dapaf-1/HAC1
  • Dark/Hac-1/dApaf-1
  • dark/hac-1/dapaf-1
  • Dark/Hac-1/dApaf1
  • Dmel\\CG6829
  • fog
  • hac-1
  • Hac-1
  • Hac-1/Dark
  • hac1
  • Hac1
  • l(2)SH0173
  • mKIAA0413
  • T1
  • zgc:136543
Epitope
AA 252-445
46
44
17
13
13
8
5
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
188
104
85
4
2
2
2
1
1
Host
Mouse
163
22
13
3
Clonality
Monoclonal
Conjugate
This APAF1 antibody is un-conjugated
3
3
2
2
2
2
2
2
2
2
2
2
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
147
60
59
42
29
22
15
8
8
5
4
Supplier
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Catalog No. ABIN968496
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.31680152 ABIN968497 IF WB Mouse IgG1 AA 252-445 Log in to see 24-Apaf 5
0.31680152 ABIN2704483 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
0.0010843921 ABIN400811 EIA IHC (fro) IF WB Mouse IgG C-Term Log in to see 2-00E-010 4
0.0010843921 ABIN265312 EIA IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0010843921 ABIN187407 IHC (fro) IF IP WB Rat IgG2a Log in to see 2E12
0.0010843921 ABIN1731833 ICC IF IHC ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.0010843921 ABIN1731834 ICC IF IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
0.0010843921 ABIN2202007 IF ELISA WB Mouse IgG C-Term Log in to see 14B2

Top referenced anti-APAF1 antibody for Immunofluorescence

Similar anti-APAF1 Antibodies

Application / Reactivity Human
ELISA 52 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 9 Antibodies
Immunofluorescence (IF) 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 58 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 41 Antibodies
Immunoprecipitation (IP) 22 Antibodies
Western Blotting (WB) 140 Antibodies

General

Antigen Apoptotic Peptidase Activating Factor 1 (APAF1) Antibodies
Epitope AA 252-445
(46), (44), (17), (13), (13), (8), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(188), (104), (85), (4), (2), (2), (2), (1), (1)
Host Mouse
(163), (22), (13), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This APAF1 antibody is un-conjugated
(3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunofluorescence (IF), Western Blotting (WB)
(147), (60), (59), (42), (29), (22), (15), (8), (8), (5), (4)
Pubmed 5 references available
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Product Details anti-APAF1 Antibody

Target Details APAF1 Application Details Handling References for anti-APAF1 Antibody (ABIN968496) Images
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human Apaf-1 aa. 252-445
Clone 24-Apaf
Isotype IgG1

Target Details APAF1

Product Details anti-APAF1 Antibody Application Details Handling References for anti-APAF1 Antibody (ABIN968496) Images back to top
Antigen
Alternative Name Apaf-1 (APAF1 Antibody Abstract)
Background The process of apoptosis requires the activation of aspartate-specific cystenine proteases in the caspase family. Group I caspases (1,4,5) cleave at (W/L)EHD tetrapeptide motifs, while group II caspases (2,3,7) cleave the DEXD tetrapeptide motif. Group III caspases (6,8,9) are activators of other caspases via cleavage of (I/V)EXD tetrapeptide sequences. Apoptotic protease-activating factor-1 (Apaf-1), cytochrome c, and dATP activate caspase-9, which in turn, initiates the post-mitochondrial-mediated caspase cascade that includes caspase-2, 3, 6, 7, 8 and 10. Apaf-1 is a soluble protein with a short N-terminal caspase recruitment domain (CARD), a central CED-4 homology domain, and 12 WD-40 repeats that may be involved in protein-protein interactions. During apoptosis, a large (700 kDa) aposome complex containing Apaf-1, cytochrome c, caspase-3, 7, and 9, and a smaller (200-300 kDa) microaposome complex containing caspase-3 and 7 exhibit higher cleavage activity than free caspase heterotetramers. Thus Apaf-1 is a component of the large aposome complex, which functions in caspase activation leading to caspase-dependent proteolytic events and apoptosis. This antibody is routinely tested by western blot analysis.
Synonyms: Apoptotic Protease Activating Factor-1
Molecular Weight 130 kDa
Research Area Apoptosis/Necrosis
Pathways p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-APAF1 Antibody Target Details APAF1 Handling References for anti-APAF1 Antibody (ABIN968496) Images back to top
Comment

Related Products: ABIN968536, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-APAF1 Antibody Target Details APAF1 Application Details References for anti-APAF1 Antibody (ABIN968496) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-APAF1 Antibody (ABIN968496)

Product Details anti-APAF1 Antibody Target Details APAF1 Application Details Handling Images back to top
Product cited in:

Li, Trudel, Wogan: "Nitric oxide-induced genotoxicity, mitochondrial damage, and apoptosis in human lymphoblastoid cells expressing wild-type and mutant p53." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 16, pp. 10364-9, 2002

Cain, Brown, Langlais, Cohen: "Caspase activation involves the formation of the aposome, a large (approximately 700 kDa) caspase-activating complex." in: The Journal of biological chemistry, Vol. 274, Issue 32, pp. 22686-92, 1999

Hu, Benedict, Ding, Núñez: "Role of cytochrome c and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis." in: The EMBO journal, Vol. 18, Issue 13, pp. 3586-95, 1999

Soengas, Alarcón, Yoshida, Giaccia, Hakem, Mak, Lowe: "Apaf-1 and caspase-9 in p53-dependent apoptosis and tumor inhibition." in: Science (New York, N.Y.), Vol. 284, Issue 5411, pp. 156-9, 1999

Slee, Harte, Kluck, Wolf, Casiano, Newmeyer, Wang, Reed, Nicholson, Alnemri, Green, Martin: "Ordering the cytochrome c-initiated caspase cascade: hierarchical activation of caspases-2, -3, -6, -7, -8, and -10 in a caspase-9-dependent manner." in: The Journal of cell biology, Vol. 144, Issue 2, pp. 281-92, 1999

Images

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Supplier Images
Western Blotting (WB) image for anti-APAF1 antibody (Apoptotic Peptidase Activating Factor 1) (AA 252-445) (ABIN968496) Western blot analysis of Apaf-1 on a human endothelial cell lysate. Lane 1: 1:250, la...
Immunofluorescence (IF) image for anti-APAF1 antibody (Apoptotic Peptidase Activating Factor 1) (AA 252-445) (ABIN968496) Immunofluorescence staining of WI-38 cells (Human lung fibroblasts, ATCC CCL-75).