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anti-Mouse (Murine) APAF1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended APAF1 Antibody (supplied by: Log in to see )

Antigen
Apoptotic Peptidase Activating Factor 1 (APAF1) Antibodies
  • 6230400I06Rik
  • anon-53Fa
  • apaf-1
  • Apaf-1
  • APAF-1
  • Apaf1
  • apaf1
  • APAF1
  • Apaf1l
  • arc
  • ARK
  • ark
  • CED4
  • CG6829
  • D-Apaf-1
  • dapaf
  • Dapaf-1
  • dAPAF-1
  • dapaf-1
  • dApaf-1
  • dApaf-1/DARK/HAC-1
  • Dapaf-1/HAC-1
  • dapaf-1L
  • dapaf-1S
  • dApaf1
  • DARK
  • dark
  • dArk
  • Dark
  • Dark/Apaf-I
  • dark/dapaf-1/hac-1
  • Dark/Dapaf-1/HAC1
  • Dark/Hac-1/dApaf-1
  • dark/hac-1/dapaf-1
  • Dark/Hac-1/dApaf1
  • Dmel\\CG6829
  • fog
  • hac-1
  • Hac-1
  • Hac-1/Dark
  • hac1
  • Hac1
  • l(2)SH0173
  • mKIAA0413
  • T1
  • zgc:136543
Epitope
N-Term
46
43
17
13
13
8
5
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
201
110
90
4
2
2
2
1
1
1
Host
Rabbit
172
25
13
4
Clonality
Polyclonal
Conjugate
This APAF1 antibody is un-conjugated
3
3
2
2
2
2
2
2
2
2
2
2
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
158
71
64
51
34
22
21
13
9
4
4
Supplier
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Catalog No. ABIN499287
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.34288716 ABIN499289 EIA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 4
0.34288716 ABIN1105393 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
0.34288716 ABIN615100 IHC (p) Rabbit C-Term Log in to see Polyclonal
0.0008982679 ABIN319003 IHC (p) WB Rabbit IgG AA 12-28 Log in to see Polyclonal 2
0.0008982679 ABIN492509 EIA IHC (p) Mouse IgG C-Term Log in to see 5-00E-011
0.0008982679 ABIN303381 IHC (p) WB Rabbit AA 12-28 Log in to see Polyclonal
0.0008982679 ABIN373041 IHC (p) Rabbit C-Term Log in to see Polyclonal
0.0008982679 ABIN724295 IF (p) IHC (p) WB Rabbit IgG AA 1200-1248, C-Term Log in to see Polyclonal
0.0008982679 ABIN724310 IF (p) IHC (p) WB Rabbit IgG AA 3-44, N-Term Log in to see Polyclonal
0.0008982679 ABIN2477458 IHC (p) WB Rabbit IgG AA 12-28 Log in to see Polyclonal 3
0.0008982679 ABIN1731836 IHC (p) Rabbit IgG C-Term Log in to see Polyclonal
0.0008982679 ABIN1858034 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 872-1135 Log in to see Polyclonal
0.0008982679 ABIN724297 IHC (p) WB Biotin Rabbit IgG AA 1200-1248, C-Term Log in to see Polyclonal
0.0008982679 ABIN724304 IHC (p) WB HRP Rabbit IgG AA 1200-1248, C-Term Log in to see Polyclonal
0.0008982679 ABIN724312 IHC (p) WB Biotin Rabbit IgG AA 3-44, N-Term Log in to see Polyclonal
0.0008982679 ABIN724319 IHC (p) WB HRP Rabbit IgG AA 3-44, N-Term Log in to see Polyclonal
0.0008982679 ABIN1731835 IHC (p) WB Rabbit IgG AA 12-28 Log in to see Polyclonal
0.0008982679 ABIN1731834 ICC IF IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
0.0008982679 ABIN4280957 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
0.0008982679 ABIN4280952 ICC IF IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal

Top referenced anti-APAF1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-APAF1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 31 Antibodies 39 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies 4 Antibodies
Immunocytochemistry (ICC) 7 Antibodies 14 Antibodies
Immunofluorescence (IF) 5 Antibodies 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies 22 Antibodies
Immunohistochemistry (IHC) 32 Antibodies 33 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antibodies 25 Antibodies
Immunoprecipitation (IP) 7 Antibodies
Western Blotting (WB) 56 Antibodies
Immunochromatography (IC) 2 Antibodies

General

Antigen Apoptotic Peptidase Activating Factor 1 (APAF1) Antibodies
Epitope N-Term
(46), (43), (17), (13), (13), (8), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(201), (110), (90), (4), (2), (2), (2), (1), (1), (1)
Host Rabbit
(172), (25), (13), (4)
Clonality
Polyclonal
Conjugate This APAF1 antibody is un-conjugated
(3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(158), (71), (64), (51), (34), (22), (21), (13), (9), (4), (4)
Pubmed 4 references available
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Product Details anti-APAF1 Antibody

Target Details APAF1 Application Details Handling References for anti-APAF1 Antibody (ABIN499287) Images
Specificity Apaf1 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human Apaf1. The sequences of the immunogenic peptide are identical between human and mouse.
Purification Affinity chromatography purified via peptide column
Immunogen Human Apaf1 (N-Terminus) Peptide
Isotype IgG

Target Details APAF1

Product Details anti-APAF1 Antibody Application Details Handling References for anti-APAF1 Antibody (ABIN499287) Images back to top
Antigen
Alternative Name APAF1 (APAF1 Antibody Abstract)
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1) (1,2). Apaf1 binds to cytochrome c (Apaf-2) and caspase-9 (Apaf-3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Apaf1 can also associate with caspase-4 and caspase-8 (4). Apaf1 is ubiquitously expressed in human tissues .Synonyms: Apaf-1, Apoptotic protease-activating factor 1, KIAA0413
Gene ID 317
UniProt O14727
Research Area Cancer, Apoptosis/Necrosis
Pathways p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-APAF1 Antibody Target Details APAF1 Handling References for anti-APAF1 Antibody (ABIN499287) Images back to top
Application Notes ELISA. Western Blot: Apaf1 antibody can be used for detection of Apaf1 at 1 - 2 μg/mL. Whole celllysate from HeLa cells can be used as positive control and a 115 - 130 kDa band should bedetected. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-APAF1 Antibody Target Details APAF1 Application Details References for anti-APAF1 Antibody (ABIN499287) Images back to top
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

References for anti-APAF1 Antibody (ABIN499287)

Product Details anti-APAF1 Antibody Target Details APAF1 Application Details Handling Images back to top
Background publications

Hu, Benedict, Wu, Inohara, Núñez: "Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 8, pp. 4386-91, 1998

Cecconi, Alvarez-Bolado, Meyer, Roth, Gruss: "Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development." in: Cell, Vol. 94, Issue 6, pp. 727-37, 1998

Zou, Henzel, Liu, Lutschg, Wang: "Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3." in: Cell, Vol. 90, Issue 3, pp. 405-13, 1997

Li, Nijhawan, Budihardjo, Srinivasula, Ahmad, Alnemri, Wang: "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade." in: Cell, Vol. 91, Issue 4, pp. 479-89, 1997

Images

Product Details anti-APAF1 Antibody Target Details APAF1 Application Details Handling References for anti-APAF1 Antibody (ABIN499287) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-APAF1 antibody (Apoptotic Peptidase Activating Factor 1) (N-Term) (ABIN499287) Immunohistochemistry of Apaf1 in human heart tissue with Apaf1 antibody at 1 μg/ml.
Western Blotting (WB) image for anti-APAF1 antibody (Apoptotic Peptidase Activating Factor 1) (N-Term) (ABIN499287) Western blot analysis of Apaf1 in K562 cell lysate with AP30055PU-N Apaf1 antibody at...