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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||27 Antibodies||35 Antibodies|
|Enzyme Immunoassay (EIA)||4 Antibodies||4 Antibodies|
|Immunocytochemistry (ICC)||2 Antibodies||9 Antibodies|
|Immunofluorescence (IF)||2 Antibodies||3 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies||22 Antibodies|
|Immunohistochemistry (IHC)||26 Antibodies||27 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||18 Antibodies||21 Antibodies|
|Immunoprecipitation (IP)||6 Antibodies|
|Western Blotting (WB)||51 Antibodies|
|Immunochromatography (IC)||2 Antibodies|
|Antigen||Apoptotic Peptidase Activating Factor 1 (APAF1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This APAF1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-APAF1 AntibodyTarget Details APAF1 Application Details Handling References for anti-APAF1 Antibody (ABIN499287) Images
|Specificity||Apaf1 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human Apaf1. The sequences of the immunogenic peptide are identical between human and mouse.|
|Purification||Affinity chromatography purified via peptide column|
|Immunogen||Human Apaf1 (N-Terminus) Peptide|
Target Details APAF1Product Details anti-APAF1 Antibody Application Details Handling References for anti-APAF1 Antibody (ABIN499287) Images back to top
|Alternative Name||APAF1 (APAF1 Antibody Abstract)|
|Background||Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1) (1,2). Apaf1 binds to cytochrome c (Apaf-2) and caspase-9 (Apaf-3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Apaf1 can also associate with caspase-4 and caspase-8 (4). Apaf1 is ubiquitously expressed in human tissues .Synonyms: Apaf-1, Apoptotic protease-activating factor 1, KIAA0413|
|Research Area||Cancer, Apoptosis/Necrosis|
|Pathways||p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-APAF1 Antibody Target Details APAF1 Handling References for anti-APAF1 Antibody (ABIN499287) Images back to top
ELISA. Western Blot: Apaf1 antibody can be used for detection of Apaf1 at 1 - 2 μg/mL. Whole celllysate from HeLa cells can be used as positive control and a 115 - 130 kDa band should bedetected. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-APAF1 Antibody Target Details APAF1 Application Details References for anti-APAF1 Antibody (ABIN499287) Images back to top
|Buffer||PBS containing 0.02 % sodium azide.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|
References for anti-APAF1 Antibody (ABIN499287)Product Details anti-APAF1 Antibody Target Details APAF1 Application Details Handling Images back to top
Cecconi, Alvarez-Bolado, Meyer et al.: "Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development." in: Cell, Vol. 94, Issue 6, pp. 727-37, 1998 (PubMed).
Hu, Benedict, Wu et al.: "Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 8, pp. 4386-91, 1998 (PubMed).
Li, Nijhawan, Budihardjo et al.: "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade." in: Cell, Vol. 91, Issue 4, pp. 479-89, 1997 (PubMed).
Zou, Henzel, Liu et al.: "Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3." in: Cell, Vol. 90, Issue 3, pp. 405-13, 1997 (PubMed).
ImagesProduct Details anti-APAF1 Antibody Target Details APAF1 Application Details Handling References for anti-APAF1 Antibody (ABIN499287) back to top