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|Antigen||Ataxia Telangiectasia Mutated (ATM) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This ATM antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
|19 references available|
|Supplier||Log in to see|
Product Details anti-ATM AntibodyTarget Details ATM Application Details Handling References for anti-ATM antibody (ABIN151772) Images
|No Cross-Reactivity||Dog (Canine)|
|Purification||Protein G purified|
|Immunogen||Synthetic peptide made to a region surrounding the phosphorylated Serine 1981 of human ATM. [UniProt# Q13315]|
Target Details ATMProduct Details anti-ATM Antibody Application Details Handling References for anti-ATM antibody (ABIN151772) Images back to top
|Alternative Name||ATM (ATM Antibody Abstract)|
|Background||Gene Symbol: ATM|
|Molecular Weight||Theoretical MW: 370 kDa|
|Research Area||DNA/RNA, Cancer|
|Pathways||p53 Signaling, Apoptosis, DNA Damage Repair, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus|
Application DetailsProduct Details anti-ATM Antibody Target Details ATM Handling References for anti-ATM antibody (ABIN151772) Images back to top
|Application Notes||Western Blot 1:1000, Immunohistochemistry 1:200, Immunocytochemistry/Immunofluorescence 1:1000, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:200This ATM [p Ser1981] (10H11.E12) antibody useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry on paraffin-embedded sections and Western blot, where a band at ~370 kDa can be seen. In IHC-P, staining was observed in the nucleus and cytoplasm of mouse spleen. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
Protocol specific for ATM Kinase Antibody Western Blot Procedure1) Resolve protein samples on a 6 % SDS-PAGE gel at 185V for ~1.5 hours.
2) Transfer to PVDF membranes at 25V for ~1.5 hours.
) Block the membrane with TBST+BSA and goat serum for 1 hour at RT.
) Dilute primary antibody to 1:1,000 in blocking buffer.
) Incubate membrane overnight at 4 degrees Celcius in diluted anti-ATM-kinase.
) Wash 3 times ten minutes on a shaker.
) Incubate membranes with HRP conjugated anti-mouse IgG for 1 hour (RT), diluted in blocking buffer.
) Wash 3 times ten minutes on a shaker.
) Add ECL reagent, as per kit directions, and expose for 1-5 seconds.
|Restrictions||For Research Use only|
HandlingProduct Details anti-ATM Antibody Target Details ATM Application Details References for anti-ATM antibody (ABIN151772) Images back to top
Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage||-80 °C,-20 °C|
|Storage Comment||Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.|
References for anti-ATM antibody (ABIN151772)Product Details anti-ATM Antibody Target Details ATM Application Details Handling Images back to top
|Product cited in:||
Liu, Liu, Gao, Reske, Li, Wu, Yang, Chen, Luo, Xiao: "A single non-synonymous NCOA5 variation in type 2 diabetic patients with hepatocellular carcinoma impairs the function of NCOA5 in cell cycle regulation." in: Cancer letters, Vol. 391, pp. 152-161, 2017 Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Human).
Wu, Wang, Chen, Chao, Chang, Chen, Hung: "Early Passage Mesenchymal Stem Cells Display Decreased Radiosensitivity and Increased DNA Repair Activity." in: Stem cells translational medicine, Vol. 6, Issue 6, pp. 1504-1514, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Human).
Takai, Jenkinson, Kabir, Babul-Hirji, Najm-Tehrani, Chitayat, Crow, de Lange: "A POT1 mutation implicates defective telomere end fill-in and telomere truncations in Coats plus." in: Genes & development, Vol. 30, Issue 7, pp. 812-26, 2016 (Sample species: Human).
Banerjee, Banerjee, Mandal: "Enhanced chemotherapeutic efficacy of apigenin liposomes in colorectal cancer based on flavone-membrane interactions." in: Journal of colloid and interface science, Vol. 491, pp. 98-110, 2016 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).
Yang, Shen, Oishi, Matteson, Tian, Goronzy, Weyand: "Restoring oxidant signaling suppresses proarthritogenic T cell effector functions in rheumatoid arthritis." in: Science translational medicine, Vol. 8, Issue 331, pp. 331ra38, 2016 Method employed by authors: Western Blotting (WB) (Sample species: Human).
Liedtke, Biebernick, Radke, Stapelkamp, Coenen, Zaehres, Fritz, Kogler: "DNA damage response in neonatal and adult stromal cells compared with induced pluripotent stem cells." in: Stem cells translational medicine, Vol. 4, Issue 6, pp. 576-89, 2015 (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence
Caserta, Egriboz, Wang, Martin, Koivisto, Pecót, Kladney, Shen, Shim, Pham, Karikomi, Mauntel, Majumder, Cuitino, Tang, Srivastava, Yu, Wallace, Mo, Park, Fernandez, Pilarski, La Perle, Rosol et al.: "Noncatalytic PTEN missense mutation predisposes to organ-selective cancer development in vivo. ..." in: Genes & development, Vol. 29, Issue 16, pp. 1707-20, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting
Sayin, Ibrahim, Larsson, Nilsson, Lindahl, Bergo: "Antioxidants accelerate lung cancer progression in mice." in: Science translational medicine, Vol. 6, Issue 221, pp. 221ra15, 2014 Further details: Western Blotting
Park, Jeon, Kim: "Selenoprotein W promotes cell cycle recovery from G2 arrest through the activation of CDC25B." in: Biochimica et biophysica acta, Vol. 1823, Issue 12, pp. 2217-26, 2012 (Sample species: Mouse (Murine)). Further details: Western Blotting
Someya, Sakata, Matsumoto, Tauchi, Kai, Hareyama, Fukushima: "Effects of depletion of dihydropyrimidine dehydrogenase on focus formation and RPA phosphorylation." in: Journal of radiation research, Vol. 53, Issue 2, pp. 250-6, 2012 (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence
Guerra, Albihn, Tronnersjö, Yan, Guidi, Stenerlöw, Sterzenbach, Josenhans, Fox, Schauer, Thelestam, Larsson, Henriksson, Frisan: "Myc is required for activation of the ATM-dependent checkpoints in response to DNA damage." in: PLoS ONE, Vol. 5, Issue 1, pp. e8924, 2010 Further details: Western Blotting
Gastaldello, Hildebrand, Faridani, Callegari, Palmkvist, Di Guglielmo, Masucci: "A deneddylase encoded by Epstein-Barr virus promotes viral DNA replication by regulating the activity of cullin-RING ligases." in: Nature cell biology, Vol. 12, Issue 4, pp. 351-61, 2010
Caslini, Connelly, Serna, Broccoli, Hess: "MLL associates with telomeres and regulates telomeric repeat-containing RNA transcription." in: Molecular and cellular biology, Vol. 29, Issue 16, pp. 4519-26, 2009
Shao, Fujii, Colmegna, Oishi, Goronzy, Weyand: "Deficiency of the DNA repair enzyme ATM in rheumatoid arthritis." in: The Journal of experimental medicine, Vol. 206, Issue 6, pp. 1435-49, 2009
Yalcin, Zhang, Luciano, Mungamuri, Marinkovic, Vercherat, Sarkar, Grisotto, Taneja, Ghaffari: "Foxo3 is essential for the regulation of ataxia telangiectasia mutated and oxidative stress-mediated homeostasis of hematopoietic stem cells." in: The Journal of biological chemistry, Vol. 283, Issue 37, pp. 25692-705, 2008
Spardy, Duensing, Hoskins, Wells, Duensing: "HPV-16 E7 reveals a link between DNA replication stress, fanconi anemia D2 protein, and alternative lengthening of telomere-associated promyelocytic leukemia bodies." in: Cancer research, Vol. 68, Issue 23, pp. 9954-63, 2008
Luo, Rosenke, Czornak, Fortunato: "Human cytomegalovirus disrupts both ataxia telangiectasia mutated protein (ATM)- and ATM-Rad3-related kinase-mediated DNA damage responses during lytic infection." in: Journal of virology, Vol. 81, Issue 4, pp. 1934-50, 2007
Bakkenist, Drissi, Wu, Kastan, Dome: "Disappearance of the telomere dysfunction-induced stress response in fully senescent cells." in: Cancer research, Vol. 64, Issue 11, pp. 3748-52, 2004
Bakkenist, Kastan: "DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation." in: Nature, Vol. 421, Issue 6922, pp. 499-506, 2003
ImagesProduct Details anti-ATM Antibody Target Details ATM Application Details Handling References for anti-ATM antibody (ABIN151772) back to top