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anti-Human ATM antibody for Immunoprecipitation

Recommended ATM Antibody (supplied by: Log in to see )

Antigen
Ataxia Telangiectasia Mutated (ATM) Antibodies
  • AI256621
  • at1
  • AT1
  • ATA
  • ATC
  • ATD
  • ATDC
  • atdc
  • ATE
  • Atm
  • ATM
  • atm
  • atm/tefu
  • C030026E19Rik
  • CG6535
  • dATM
  • Dmel\\CG6535
  • tef
  • Tefu
  • tel1
  • TEL1
  • TELO1
  • telo1
  • Xatm
Epitope
pSer1981
68
28
17
13
13
13
7
7
7
5
5
4
4
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
280
74
28
6
4
1
1
Host
Mouse
205
52
14
8
3
Clonality
Monoclonal
Conjugate
This ATM antibody is un-conjugated
8
8
7
7
6
6
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
157
78
72
50
40
36
23
22
16
11
6
4
4
2
2
1
1
1
1
Supplier
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Available images

Catalog No. ABIN251422
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089235755 ABIN151738 IP WB Goat Log in to see Polyclonal 1
0.00089235755 ABIN189039 ICC IF IHC (p) IP IHC WB Rabbit IgG Ser1981 Log in to see Y170
0.00089235755 ABIN302280 IHC (p) IP WB Mouse IgG1 AA 980-1512 Log in to see 3-00E-008
0.00089235755 ABIN258963 ICC IF IHC (p) IP IHC WB Rabbit IgG Ser1981 Log in to see EP1890Y
0.00089235755 ABIN214233 IHC (p) IP WB Mouse IgG1 AA 980-1512 Log in to see 3E8
0.00089235755 ABIN2469773 IP IHC WB Mouse IgG1 AA 980-1152 Log in to see 3-00E-008
0.00089235755 ABIN331236 IP ELISA WB Mouse IgG2b, kappa Log in to see
0.00089235755 ABIN331246 IP ELISA WB Mouse IgG2b, kappa Log in to see
0.00089235755 ABIN152102 ICC IF IHC (p) IP IHC PLA WB Rabbit AA 2550-2600 Log in to see Polyclonal
0.00089235755 ABIN151775 ICC IF IHC (p) IP IHC ELISA WB Mouse IgG1 AA 2577-3056 Log in to see 2C1
0.00089235755 ABIN221982 IP WB Mouse IgG Log in to see
0.00089235755 ABIN221229 IF IP IHC WB Mouse IgG1 AA 2577-3056 Log in to see 2C1
0.00089235755 ABIN223482 IP WB Rabbit IgG Log in to see Polyclonal
0.00089235755 ABIN363380 ICC FACS IF IP IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.00089235755 ABIN2617305 IF IHC (p) IP WB Mouse IgG1 AA 2577-3056 Log in to see 2C1
0.00089235755 ABIN342243 IP WB Mouse IgG1 AA 2577-3056 Log in to see
0.00089235755 ABIN242149 IP WB Rabbit Log in to see Polyclonal
0.00089235755 ABIN1723092 IHC (p) IP WB Mouse IgG1 Log in to see 3E8
0.00089235755 ABIN1976851 IP WB Goat IgG AA 1750-1800 Log in to see Polyclonal
0.00089235755 ABIN1976854 IP PLA WB Goat IgG AA 2550-2600 Log in to see Polyclonal

Top referenced anti-ATM antibody for Immunoprecipitation

Similar anti-ATM Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Chromatin Immunoprecipitation (ChIP) 1 Antibodies 1 Antibodies
ELISA 2 Antibodies 9 Antibodies
Flow Cytometry (FACS) 1 Antibodies 4 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 13 Antibodies
Immunofluorescence (IF) 4 Antibodies 20 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies 12 Antibodies
Immunohistochemistry (IHC) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Western Blotting (WB) 11 Antibodies
Biochemical Assay (BCA) 2 Antibodies
BioImaging (BI) 1 Antibodies

General

Antigen Ataxia Telangiectasia Mutated (ATM) Antibodies
Epitope pSer1981
(68), (28), (17), (13), (13), (13), (7), (7), (7), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(280), (74), (28), (6), (4), (1), (1)
Host Mouse
(205), (52), (14), (8), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ATM antibody is un-conjugated
(8), (8), (7), (7), (6), (6), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(157), (78), (72), (50), (40), (36), (23), (22), (16), (11), (6), (4), (4), (2), (2), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-ATM Antibody

Target Details ATM Application Details Handling References for anti-ATM Antibody (ABIN251422) Images
No Cross-Reactivity Dog (Canine)
Cross-Reactivity (Details) Does not work in canine per customer review
Purification Protein G purified
Immunogen Synthetic peptide made to a region surrounding the phosphorylated Serine 1981 of human ATM.
Clone 10H11-E12
Isotype IgG1 kappa

Target Details ATM

Product Details anti-ATM Antibody Application Details Handling References for anti-ATM Antibody (ABIN251422) Images back to top
Antigen
Alternative Name ATM (ATM Antibody Abstract)
Background ATM-kinase plays an important regulatory role. DNA damage caused by such things asionizing irradiation activates ATM-kinase, leading to a cascade of kinase reactions thatregulate cell cycle, apoptosis, and DNA damage repair. Alternate Names: anti-phosphorylated ATM antibody, anti-ATM S1981 antibody, anti-ATM phosphorylatedSer 1981 antibody, anti-ATM (phospho S1981) antibody.
Gene Symbol: ATM
Gene ID 472, 300711
UniProt Q13315
Pathways p53 Signaling, Apoptosis, DNA Damage Repair

Application Details

Product Details anti-ATM Antibody Target Details ATM Handling References for anti-ATM Antibody (ABIN251422) Images back to top
Application Notes This ATM [p Ser1981] (10H11.E12) antibody useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry on paraffin-embedded sections and Western blot, where a band at ~370 kDa can be seen. In IHC-P, staining was observed in the nucleus and cytoplasm of mouse spleen.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:1000, Immunohistochemistry 1:200, Immunohistochemistry-Paraffin 1:200, Immunoprecipitation 1:10-1:500, Western Blot 1:1000
Protocol Protocol specific for ATM Kinase Antibody Western Blot Procedure1) Resolve protein samples on a 6 % SDS-PAGE gel at 185V for ~1.5 hours.
2) Transfer to PVDF membranes at 25V for ~1.5 hours.
) Block the membrane with TBST+BSA and goat serum for 1 hour at RT.
) Dilute primary antibody to 1:1,000 in blocking buffer.
) Incubate membrane overnight at 4 degrees Celcius in diluted anti-ATM-kinase.
) Wash 3 times ten minutes on a shaker.
) Incubate membranes with HRP conjugated anti-mouse IgG for 1 hour (RT), diluted in blocking buffer.
) Wash 3 times ten minutes on a shaker.
) Add ECL reagent, as per kit directions, and expose for 1-5 seconds.
Restrictions For Research Use only

Handling

Product Details anti-ATM Antibody Target Details ATM Application Details References for anti-ATM Antibody (ABIN251422) Images back to top
Format Liquid
Concentration 0.97 mg/mL
Buffer Tris-glycine, 150 mM NaCl, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.

References for anti-ATM Antibody (ABIN251422)

Product Details anti-ATM Antibody Target Details ATM Application Details Handling Images back to top
Product cited in:

Yalcin, Zhang, Luciano, Mungamuri, Marinkovic, Vercherat, Sarkar, Grisotto, Taneja, Ghaffari: "Foxo3 is essential for the regulation of ataxia telangiectasia mutated and oxidative stress-mediated homeostasis of hematopoietic stem cells." in: The Journal of biological chemistry, Vol. 283, Issue 37, pp. 25692-705, 2008

Spardy, Duensing, Hoskins, Wells, Duensing: "HPV-16 E7 reveals a link between DNA replication stress, fanconi anemia D2 protein, and alternative lengthening of telomere-associated promyelocytic leukemia bodies." in: Cancer research, Vol. 68, Issue 23, pp. 9954-63, 2008

Bakkenist, Kastan: "DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation." in: Nature, Vol. 421, Issue 6922, pp. 499-506, 2003

Images

Product Details anti-ATM Antibody Target Details ATM Application Details Handling References for anti-ATM Antibody (ABIN251422) back to top
Supplier Images
Western Blotting (WB) image for anti-ATM antibody (Ataxia Telangiectasia Mutated) (pSer1981) (ABIN251422) Western Blot analysis of ATM-kinase, using ABIN151772. Sample: Irradiated or peroxida...