anti-Mouse (Murine) GTPase NRas antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended GTPase NRas Antibody (supplied by: Log in to see )

Antigen
GTPase NRas (NRAS) Antibodies
  • NRAS
  • AV095280
  • N-ras
  • ALPS4
  • NRAS1
  • NS6
  • N-RAS
  • fj99a10
  • wu:fj99a10
  • zgc:100761
  • zras-b1
  • H-RAS
  • HRAS
  • GTPase NRas
  • African clawed frog N-ras
  • neuroblastoma ras oncogene
  • neuroblastoma RAS viral (v-ras) oncogene homolog
  • Tsp_01117
  • n-ras
  • Nras
  • NRAS
  • nras
Epitope
AA 167-179
23
9
8
7
6
5
5
4
2
2
1
1
1
1
Reactivity
Bat, Cow (Bovine), Dog (Canine), Horse (Equine), Human, Mouse (Murine), Pig (Porcine)
101
28
17
5
2
2
2
2
2
1
1
1
Host
Goat
45
37
19
Clonality
Polyclonal
Conjugate
This GTPase NRas antibody is un-conjugated
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
88
58
29
23
20
20
18
5
2
1
1
1
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Catalog No. ABIN337227
$ 451.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.5717 ABIN738336 IF (p) IHC (p) WB Rabbit IgG AA 166-186 Log in to see Polyclonal
1 ABIN5553509 IHC (p) WB Goat Internal Region Log in to see Polyclonal
1 ABIN1493893 IHC (p) WB Rabbit AA 91-140 Log in to see Polyclonal
1 ABIN372821 EIA IHC (p) WB Goat IgG Internal Region Log in to see Polyclonal
1 ABIN1918485 IHC (p) ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN1737523 IHC (p) ELISA WB Goat Internal Region Log in to see Polyclonal
1 ABIN2963636 IHC (p) ELISA WB Rabbit IgG C-Term Log in to see Polyclonal

General

Antigen GTPase NRas (NRAS) Antibodies
Epitope AA 167-179
(23), (9), (8), (7), (6), (5), (5), (4), (2), (2), (1), (1), (1), (1)
Reactivity Bat, Cow (Bovine), Dog (Canine), Horse (Equine), Human, Mouse (Murine), Pig (Porcine)
(101), (28), (17), (5), (2), (2), (2), (2), (2), (1), (1), (1)
Host Goat
(45), (37), (19)
Clonality
Polyclonal
Conjugate This GTPase NRas antibody is un-conjugated
(2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(88), (58), (29), (23), (20), (20), (18), (5), (2), (1), (1), (1)
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Product Details anti-GTPase NRas Antibody

Target Details GTPase NRas Application Details Handling Images
Brand IHC-plus™
Specificity Human NRAS.
Predicted Reactivity Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Mouse, Elephant, Panda, Bovine, Dog, Bat, Horse, Pig, Opossum (100%) Marmoset, Rat, Hamster, Guinea pig (92%).
Purification Immunoaffinity purified
Immunogen Synthetic peptide C-RMKKLNSSDDGTQ from an internal region of human NRAS (NP_002515.1). Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Mouse, Elephant, Panda, Bovine, Dog, Bat, Horse, Pig, Opossum (100%), Marmoset, Rat, Hamster, Guinea pig (92%).

Type of Immunogen: Synthetic peptide

Target Details GTPase NRas

Product Details anti-GTPase NRas Antibody Application Details Handling Images back to top
Antigen
Alternative Name NRAS / N-ras (NRAS Antibody Abstract)
Background Name/Gene ID: NRAS
Subfamily: RAS oncogene
Family: Ras GTPase superfamily IPR001806

Synonyms: NRAS, HRAS1, NRAS1, NS6, ALPS4, Transforming protein N-Ras, N-ras, N-ras protein part 4, GTPase NRas
Gene ID 4893
NCBI Accession NP_002515
UniProt P01111
Pathways p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling

Application Details

Product Details anti-GTPase NRas Antibody Target Details GTPase NRas Handling Images back to top
Application Notes Approved: ELISA (1:32000), IHC-P (2.5 μg/mL), WB (1:32000, 0.01 - 0.03 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.
Restrictions For Research Use only

Handling

Product Details anti-GTPase NRas Antibody Target Details GTPase NRas Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Store at -20°C. Minimize freezing and thawing.

Images

Product Details anti-GTPase NRas Antibody Target Details GTPase NRas Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-GTPase NRas (NRAS) (AA 167-179) antibody (ABIN337227) Human Small Intestine (formalin-fixed, paraffin-embedded) stained with x antibody ABI...