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anti-Human PAG1 antibody for Flow Cytometry

Recommended PAG1 Antibody (supplied by: Log in to see )

Antigen
phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) Antibodies
  • 8430414N03Rik
  • CBP
  • Cbp
  • F730007C19Rik
  • IGFBP-4ase
  • MGC80192
  • PAG
  • Pag
  • PAG1
  • PAPP-A
Epitope
AA 97-432
34
20
13
3
2
1
1
1
Reactivity
Human
89
48
40
23
Host
Mouse
55
38
Clonality
Monoclonal
Conjugate
This PAG1 antibody is un-conjugated
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
69
54
28
21
20
11
3
1
Supplier
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Catalog No. ABIN120652
$ 308.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010485112 ABIN94272 FACS IHC (p) WB Mouse IgG2a AA 97-432 Log in to see MEM-255 6
0.0010485112 ABIN2470739 FACS IHC WB Mouse IgG2a AA 97-432 Log in to see MEM-255
0.0010485112 ABIN351517 FACS IHC (p) WB Mouse IgG2a AA 97-432 Log in to see MEM-255
0.0010485112 ABIN1723397 FACS IHC (p) WB Mouse IgG2a AA 97-432 Log in to see MEM-255
0.0010485112 ABIN268975 FACS IHC (p) IHC WB Mouse IgG2a AA 235-280 Log in to see MEM-255
0.0010485112 ABIN4343398 FACS IHC IHC (p) WB DyLight 350 Mouse IgG2a AA 235-280 Log in to see MEM-255
0.0010485112 ABIN4343399 FACS IHC IHC (p) WB DyLight 405 Mouse IgG2a AA 235-280 Log in to see MEM-255
0.0010485112 ABIN4343400 FACS IHC IHC (p) WB PE Mouse IgG2a AA 235-280 Log in to see MEM-255
0.0010485112 ABIN4343401 FACS IHC IHC (p) WB PerCP Mouse IgG2a AA 235-280 Log in to see MEM-255
0.0010485112 ABIN4343402 FACS IHC IHC (p) WB Allophycocyanin Mouse IgG2a AA 235-280 Log in to see MEM-255
0.0010485112 ABIN4343403 FACS IHC IHC (p) WB DyLight 755 Mouse IgG2a AA 235-280 Log in to see MEM-255
0.0010485112 ABIN4343404 FACS IHC IHC (p) WB DyLight 680 Mouse IgG2a AA 235-280 Log in to see MEM-255
0.0010485112 ABIN4343405 FACS IHC IHC (p) WB DyLight 488 Mouse IgG2a AA 235-280 Log in to see MEM-255
0.0010485112 ABIN4343406 FACS IHC IHC (p) WB DyLight 405LS Mouse IgG2a AA 235-280 Log in to see MEM-255
0.0010485112 ABIN4343407 FACS IHC IHC (p) WB Alexa Fluor 405 Mouse IgG2a AA 235-280 Log in to see MEM-255
0.0010485112 ABIN4343408 FACS IHC IHC (p) WB Alexa Fluor 488 Mouse IgG2a AA 235-280 Log in to see MEM-255
0.0010485112 ABIN4343409 FACS IHC IHC (p) WB Alexa Fluor 647 Mouse IgG2a AA 235-280 Log in to see MEM-255
0.0010485112 ABIN4343410 FACS IHC IHC (p) WB Alexa Fluor 700 Mouse IgG2a AA 235-280 Log in to see MEM-255
0.0010485112 ABIN322867 FACS WB Mouse IgG2a AA 97-432 Log in to see
0.0010485112 ABIN2329460 FACS IHC WB Mouse IgG2a AA 97-432 Log in to see MEM-255

Top referenced anti-PAG1 antibody for Flow Cytometry

Similar anti-PAG1 Antibodies

Application / Reactivity Human
ELISA 1 Antibodies
Flow Cytometry (FACS) 22 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 24 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 58 Antibodies
Immunoprecipitation (IP) 31 Antibodies
Western Blotting (WB) 72 Antibodies

General

Antigen phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) Antibodies
Epitope AA 97-432
(34), (20), (13), (3), (2), (1), (1), (1)
Reactivity Human
(89), (48), (40), (23)
Host Mouse
(55), (38)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PAG1 antibody is un-conjugated
(3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(69), (54), (28), (21), (20), (11), (3), (1)
Pubmed 1 reference available
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Product Details anti-PAG1 Antibody

Target Details PAG1 Application Details Handling References for anti-PAG1 Antibody (ABIN120652) Images
Specificity The antibody recognizes an epitope (aa 235-280) of Csk-binding protein (Cbp) located in the cytoplasmic domain, also known as protein associated with glycosphingolipid-enriched microdomains (PAG).
Purification Protein-A affinity chromatography
Purity > 95 % pure (by SDS-PAGE)
Immunogen Recombinant intracellular fragment (aa 97-432) of human Cbp/PAG
Clone MEM-255
Isotype IgG2a

Target Details PAG1

Product Details anti-PAG1 Antibody Application Details Handling References for anti-PAG1 Antibody (ABIN120652) Images back to top
Antigen
Alternative Name PAG1 / Csk-Binding Protein (PAG1 Antibody Abstract)
Background PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.Synonyms: CBP, PAG, Phosphoprotein associated with glycosphingolipid-enriched microdomains 1, Transmembrane adapter protein PAG, Transmembrane phosphoprotein Cbp
Gene ID 55824
UniProt Q9NWQ8
Pathways p53 Signaling, EGFR Signaling Pathway

Application Details

Product Details anti-PAG1 Antibody Target Details PAG1 Handling References for anti-PAG1 Antibody (ABIN120652) Images back to top
Application Notes Flow Cytometry: 2 mg/mL (intracellular staining, permeabilization required). Western Blotting: Csk binding protein is an ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane microdomains (rafts), which however migrates on SDS- PAGE gels anomalously as an 80 kDa molecule. Immunohistochemistry (paraffin sections).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PAG1 Antibody Target Details PAG1 Application Details References for anti-PAG1 Antibody (ABIN120652) Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.
Expiry Date 12 months

References for anti-PAG1 Antibody (ABIN120652)

Product Details anti-PAG1 Antibody Target Details PAG1 Application Details Handling Images back to top
Background publications

Brdicková, Brdicka, Andera, Spicka, Angelisová, Milgram, Horejsí: "Interaction between two adapter proteins, PAG and EBP50: a possible link between membrane rafts and actin cytoskeleton." in: FEBS letters, Vol. 507, Issue 2, pp. 133-6, 2001