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|Antigen||phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) Antibodies|
|Epitope||AA 97-432 Alternatives|
|Conjugate||This PAG1 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|1 reference available|
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Product Details anti-PAG1 AntibodyTarget Details PAG1 Application Details Handling References for anti-PAG1 antibody (ABIN120652) Images
|Specificity||The antibody recognizes an epitope (aa 235-280) of Csk-binding protein (Cbp) located in the cytoplasmic domain, also known as protein associated with glycosphingolipid-enriched microdomains (PAG).|
|Purification||Protein-A affinity chromatography|
|Purity||> 95 % pure (by SDS-PAGE)|
|Immunogen||Recombinant intracellular fragment (aa 97-432) of human Cbp/PAG|
Target Details PAG1Product Details anti-PAG1 Antibody Application Details Handling References for anti-PAG1 antibody (ABIN120652) Images back to top
|Alternative Name||PAG1 / Csk-Binding Protein (PAG1 Antibody Abstract)|
|Background||PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.Synonyms: CBP, PAG, Phosphoprotein associated with glycosphingolipid-enriched microdomains 1, Transmembrane adapter protein PAG, Transmembrane phosphoprotein Cbp|
|Pathways||p53 Signaling, TCR Signaling, EGFR Signaling Pathway, CXCR4-mediated Signaling Events|
Application DetailsProduct Details anti-PAG1 Antibody Target Details PAG1 Handling References for anti-PAG1 antibody (ABIN120652) Images back to top
Flow Cytometry: 2 mg/mL (intracellular staining, permeabilization required). Western Blotting: Csk binding protein is an ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane microdomains (rafts), which however migrates on SDS- PAGE gels anomalously as an 80 kDa molecule. Immunohistochemistry (paraffin sections).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PAG1 Antibody Target Details PAG1 Application Details References for anti-PAG1 antibody (ABIN120652) Images back to top
|Buffer||Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.
|Expiry Date||12 months|
References for anti-PAG1 antibody (ABIN120652)Product Details anti-PAG1 Antibody Target Details PAG1 Application Details Handling Images back to top
Brdicková, Brdicka, Andera, Spicka, Angelisová, Milgram, Horejsí: "Interaction between two adapter proteins, PAG and EBP50: a possible link between membrane rafts and actin cytoskeleton." in: FEBS letters, Vol. 507, Issue 2, pp. 133-6, 2001